Near infrared FRET using wide-field fluorescence lifetime imaging in live animals

2013 ◽  
Author(s):  
Lingling Zhao ◽  
Ken Abe ◽  
Margarida Barroso ◽  
Xavier Intes
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Macroscopic fluorescence-lifetime imaging of NADH and protoporphyrin IX improves the detection and grading of 5-aminolevulinic acid-stained brain tumors

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mikael T. Erkkilä ◽  
David Reichert ◽  
Johanna Gesperger ◽  
Barbara Kiesel ◽  
Thomas Roetzer ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Brain Tumors ◽  
Fluorescence Lifetime ◽  
Tumor Tissue ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Low Grade ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

AbstractMaximal safe tumor resection remains the key prognostic factor for improved prognosis in brain tumor patients. Despite 5-aminolevulinic acid-based fluorescence guidance the neurosurgeon is, however, not able to visualize most low-grade gliomas (LGG) and infiltration zone of high-grade gliomas (HGG). To overcome the need for a more sensitive visualization, we investigated the potential of macroscopic, wide-field fluorescence lifetime imaging of nicotinamide adenine dinucleotide (NADH) and protoporphyrin IX (PPIX) in selected human brain tumors. For future intraoperative use, the imaging system offered a square field of view of 11 mm at 250 mm free working distance. We performed imaging of tumor tissue ex vivo, including LGG and HGG as well as brain metastases obtained from 21 patients undergoing fluorescence-guided surgery. Half of all samples showed visible fluorescence during surgery, which was associated with significant increase in PPIX fluorescence lifetime. While the PPIX lifetime was significantly different between specific tumor tissue types, the NADH lifetimes did not differ significantly among them. However, mainly necrotic areas exhibited significantly lower NADH lifetimes compared to compact tumor in HGG. Our pilot study indicates that combined fluorescence lifetime imaging of NADH/PPIX represents a sensitive tool to visualize brain tumor tissue not detectable with conventional 5-ALA fluorescence.

scholarly journals Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination

2017 ◽  
Vol 8 (3) ◽  
pp. 1455 ◽  
Author(s):  
Taylor Hinsdale ◽  
Cory Olsovsky ◽  
Jose J. Rico-Jimenez ◽  
Kristen C. Maitland ◽  
Javier A. Jo ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Structured Illumination ◽  
Lifetime Imaging

scholarly journals Fluorescence lifetime imaging of upper gastrointestinal pH in vivo with a lanthanide based near-infrared τ probe

2019 ◽  
Vol 10 (15) ◽  
pp. 4227-4235 ◽  
Author(s):  
Yingying Ning ◽  
Shengming Cheng ◽  
Jing-Xiang Wang ◽  
Yi-Wei Liu ◽  
Wei Feng ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Lanthanide Complex ◽  
Fluorescence Lifetime Imaging ◽  
Ph Responsive ◽  
Gastrointestinal Ph ◽  
Lifetime Imaging ◽  
Upper Gastrointestinal

Lanthanide complex was successfully applied in the design of pH-responsive NIR τ probe for quantitative in vivo imaging.

High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging

2004 ◽  
Vol 29 (19) ◽  
pp. 2249 ◽  
Author(s):  
J. Requejo-Isidro ◽  
J. McGinty ◽  
I. Munro ◽  
D. S. Elson ◽  
N. P. Galletly ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence lifetime imaging with near-infrared dyes

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Wolfgang Becker ◽  
Vladislav Shcheslavskiy
Keyword(s):  
Fluorescence Lifetime ◽  
Laser Scanning ◽  
Near Infrared ◽  
Single Photon ◽  
Optical Tomography ◽  
Small Animal Imaging ◽  
Supplementary Information ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Nir Dyes

AbstractNear-infrared (NIR) dyes are used as fluorescence markers in small animal imaging and in diffuse optical tomography. In these applications it is important to know whether the dyes bind to proteins or to other tissue constituents, and whether their fluorescence lifetimes depend on the targets they bind to. Unfortunately, neither the optical beam paths nor the detectors of commonly used in confocal and multiphoton laser scanning microscopes (LSMs) directly allow for excitation and detection of NIR fluorescence. This paper presents three ways of adapting existing LSMs with time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) systems for NIR FLIM: 1) confocal systems with wideband beamsplitters and diode laser excitation, 2) confocal systems with wideband beamsplitters and one-photon excitation by titanium-sapphire lasers, and 3) two-photon systems with optical parametric oscillator (OPO) excitation and non-descanned detection. A number of NIR dyes are tested in biological tissue. All of them show clear lifetime changes depending on the tissue structures they are bound to. We therefore believe that NIR FLIM can deliver supplementary information about the tissue composition and on local biochemical parameters.

scholarly journals Wide-field fluorescence lifetime imaging with optical sectioning and spectral resolution applied to biological samples

2002 ◽  
Vol 49 (5-6) ◽  
pp. 985-995 ◽  
Author(s):  
D. S. Elson ◽  
J. Siegel ◽  
S. E. D. Webb ◽  
S. Lévêquefort ◽  
D. Parsons-Karavassilis ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Spectral Resolution ◽  
Biological Samples ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Optical Sectioning ◽  
Lifetime Imaging
Sign in / Sign up

Export Citation Format

Share Document