Fluorescence lifetime imaging with near-infrared dyes

2013 ◽  
Author(s):  
Wolfgang Becker ◽  
Vladislav Shcheslavskiy
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Fluorescence lifetime imaging of upper gastrointestinal pH in vivo with a lanthanide based near-infrared τ probe

2019 ◽  
Vol 10 (15) ◽  
pp. 4227-4235 ◽  
Author(s):  
Yingying Ning ◽  
Shengming Cheng ◽  
Jing-Xiang Wang ◽  
Yi-Wei Liu ◽  
Wei Feng ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Lanthanide Complex ◽  
Fluorescence Lifetime Imaging ◽  
Ph Responsive ◽  
Gastrointestinal Ph ◽  
Lifetime Imaging ◽  
Upper Gastrointestinal

Lanthanide complex was successfully applied in the design of pH-responsive NIR τ probe for quantitative in vivo imaging.

Fluorescence lifetime imaging with near-infrared dyes

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Wolfgang Becker ◽  
Vladislav Shcheslavskiy
Keyword(s):  
Fluorescence Lifetime ◽  
Laser Scanning ◽  
Near Infrared ◽  
Single Photon ◽  
Optical Tomography ◽  
Small Animal Imaging ◽  
Supplementary Information ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Nir Dyes

AbstractNear-infrared (NIR) dyes are used as fluorescence markers in small animal imaging and in diffuse optical tomography. In these applications it is important to know whether the dyes bind to proteins or to other tissue constituents, and whether their fluorescence lifetimes depend on the targets they bind to. Unfortunately, neither the optical beam paths nor the detectors of commonly used in confocal and multiphoton laser scanning microscopes (LSMs) directly allow for excitation and detection of NIR fluorescence. This paper presents three ways of adapting existing LSMs with time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) systems for NIR FLIM: 1) confocal systems with wideband beamsplitters and diode laser excitation, 2) confocal systems with wideband beamsplitters and one-photon excitation by titanium-sapphire lasers, and 3) two-photon systems with optical parametric oscillator (OPO) excitation and non-descanned detection. A number of NIR dyes are tested in biological tissue. All of them show clear lifetime changes depending on the tissue structures they are bound to. We therefore believe that NIR FLIM can deliver supplementary information about the tissue composition and on local biochemical parameters.

scholarly journals Fast fit-free analysis of fluorescence lifetime imaging via deep learning

2019 ◽  
Vol 116 (48) ◽  
pp. 24019-24030 ◽  
Author(s):  
Jason T. Smith ◽  
Ruoyang Yao ◽  
Nattawut Sinsuebphon ◽  
Alena Rudkouskaya ◽  
Nathan Un ◽  
...  
Keyword(s):  
Deep Learning ◽  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Quantitative Information ◽  
Fluorescence Lifetime Imaging ◽  
Complex Data ◽  
Lifetime Imaging ◽  
Spatially Resolved ◽  
Lifetime Studies ◽  
Net Framework

Fluorescence lifetime imaging (FLI) provides unique quantitative information in biomedical and molecular biology studies but relies on complex data-fitting techniques to derive the quantities of interest. Herein, we propose a fit-free approach in FLI image formation that is based on deep learning (DL) to quantify fluorescence decays simultaneously over a whole image and at fast speeds. We report on a deep neural network (DNN) architecture, named fluorescence lifetime imaging network (FLI-Net) that is designed and trained for different classes of experiments, including visible FLI and near-infrared (NIR) FLI microscopy (FLIM) and NIR gated macroscopy FLI (MFLI). FLI-Net outputs quantitatively the spatially resolved lifetime-based parameters that are typically employed in the field. We validate the utility of the FLI-Net framework by performing quantitative microscopic and preclinical lifetime-based studies across the visible and NIR spectra, as well as across the 2 main data acquisition technologies. These results demonstrate that FLI-Net is well suited to accurately quantify complex fluorescence lifetimes in cells and, in real time, in intact animals without any parameter settings. Hence, FLI-Net paves the way to reproducible and quantitative lifetime studies at unprecedented speeds, for improved dissemination and impact of FLI in many important biomedical applications ranging from fundamental discoveries in molecular and cellular biology to clinical translation.

scholarly journals In Vivo Dendritic Cell Tracking Using Fluorescence Lifetime Imaging and Near-Infrared-Emissive Polymersomes

2009 ◽  
Vol 11 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Natalie A. Christian ◽  
Fabian Benencia ◽  
Michael C. Milone ◽  
Guizhi Li ◽  
Paul R. Frail ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Fluorescence Lifetime ◽  
Cell Tracking ◽  
Near Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

One-Pot Synthesis of Near-Infrared Fluorescent Gold Clusters for Cellular Fluorescence Lifetime Imaging

Small ◽  
2011 ◽  
Vol 7 (18) ◽  
pp. 2614-2620 ◽  
Author(s):  
Li Shang ◽  
Naghmeh Azadfar ◽  
Florian Stockmar ◽  
Winfried Send ◽  
Vanessa Trouillet ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Gold Clusters ◽  
Fluorescence Lifetime Imaging ◽  
One Pot ◽  
Lifetime Imaging ◽  
One Pot Synthesis

scholarly journals Photophysical Properties and Fluorescence Lifetime Imaging of Exfoliated Near-Infrared Fluorescent Silicate Nanosheets

2021 ◽  
Author(s):  
Gabriele Selvaggio ◽  
Milan Weitzel ◽  
Nazar Oleksiievets ◽  
Tabea Anne Oswald ◽  
Robert Nißler ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Bulk Material ◽  
Photophysical Properties ◽  
Layered Silicates ◽  
Fluorescence Lifetime Imaging ◽  
Egyptian Blue ◽  
Lifetime Imaging

The layered silicates Egyptian Blue (CaCuSi4O10, EB), Han Blue (BaCuSi4O10, HB) and Han Purple (BaCuSi2O6, HP) emit as bulk material bright and stable fluorescence in the near-infrared (NIR), which is...

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