High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging

2004 ◽  
Vol 29 (19) ◽  
pp. 2249 ◽  
Author(s):  
J. Requejo-Isidro ◽  
J. McGinty ◽  
I. Munro ◽  
D. S. Elson ◽  
N. P. Galletly ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

High-speed wide-field fluorescence lifetime imaging

2004 ◽  
Author(s):  
D.S. Elson ◽  
J. Requejo-Isidro ◽  
I. Munro ◽  
J. McGinty ◽  
C.W. Dunsby ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Wide-field Fluorescence Lifetime Imaging Microscopy with a High-Speed Mega-pixel SPAD Camera

2020 ◽  
Author(s):  
V. Zickus ◽  
M.-L. Wu ◽  
K. Morimoto ◽  
V. Kapitany ◽  
A. Fatima ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Single Photon ◽  
Fluorescent Proteins ◽  
Cell Metabolism ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Protein Activity ◽  
Lifetime Imaging

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a relatively large number of photons, hence slowing down total acquisition times. Moreover, there are many cases, for example in studies of cell collectives, where wide-field imaging is desired. We report scan-less wide-field FLIM based on a 0.5 Megapixel resolution, time-gated Single Photon Avalanche Diode (SPAD) camera, with acquisition rates up to 1 Hz. Fluorescence lifetime estimation is performed via a pre-trained artificial neural network with 1000-fold improvement in processing times compared to standard least squares fitting techniques. We utilised our system to image HT1080 – human fibrosarcoma cell line as well as Convallaria. The results show promise for real-time FLIM and a viable route towards multi-megapixel fluorescence lifetime images, with a proof-of-principle mosaic image shown with 3.6 megapixels.

scholarly journals High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy

Optica ◽  
2018 ◽  
Vol 5 (10) ◽  
pp. 1290 ◽  
Author(s):  
Andrew J. Bower ◽  
Joanne Li ◽  
Eric J. Chaney ◽  
Marina Marjanovic ◽  
Darold R. Spillman ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
High Speed Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Macroscopic fluorescence-lifetime imaging of NADH and protoporphyrin IX improves the detection and grading of 5-aminolevulinic acid-stained brain tumors

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mikael T. Erkkilä ◽  
David Reichert ◽  
Johanna Gesperger ◽  
Barbara Kiesel ◽  
Thomas Roetzer ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Brain Tumors ◽  
Fluorescence Lifetime ◽  
Tumor Tissue ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Low Grade ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

AbstractMaximal safe tumor resection remains the key prognostic factor for improved prognosis in brain tumor patients. Despite 5-aminolevulinic acid-based fluorescence guidance the neurosurgeon is, however, not able to visualize most low-grade gliomas (LGG) and infiltration zone of high-grade gliomas (HGG). To overcome the need for a more sensitive visualization, we investigated the potential of macroscopic, wide-field fluorescence lifetime imaging of nicotinamide adenine dinucleotide (NADH) and protoporphyrin IX (PPIX) in selected human brain tumors. For future intraoperative use, the imaging system offered a square field of view of 11 mm at 250 mm free working distance. We performed imaging of tumor tissue ex vivo, including LGG and HGG as well as brain metastases obtained from 21 patients undergoing fluorescence-guided surgery. Half of all samples showed visible fluorescence during surgery, which was associated with significant increase in PPIX fluorescence lifetime. While the PPIX lifetime was significantly different between specific tumor tissue types, the NADH lifetimes did not differ significantly among them. However, mainly necrotic areas exhibited significantly lower NADH lifetimes compared to compact tumor in HGG. Our pilot study indicates that combined fluorescence lifetime imaging of NADH/PPIX represents a sensitive tool to visualize brain tumor tissue not detectable with conventional 5-ALA fluorescence.

scholarly journals Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination

2017 ◽  
Vol 8 (3) ◽  
pp. 1455 ◽  
Author(s):  
Taylor Hinsdale ◽  
Cory Olsovsky ◽  
Jose J. Rico-Jimenez ◽  
Kristen C. Maitland ◽  
Javier A. Jo ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Structured Illumination ◽  
Lifetime Imaging

scholarly journals Wide-field fluorescence lifetime imaging with optical sectioning and spectral resolution applied to biological samples

2002 ◽  
Vol 49 (5-6) ◽  
pp. 985-995 ◽  
Author(s):  
D. S. Elson ◽  
J. Siegel ◽  
S. E. D. Webb ◽  
S. Lévêquefort ◽  
D. Parsons-Karavassilis ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Spectral Resolution ◽  
Biological Samples ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Optical Sectioning ◽  
Lifetime Imaging

A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning

2002 ◽  
Vol 73 (4) ◽  
pp. 1898-1907 ◽  
Author(s):  
S. E. D. Webb ◽  
Y. Gu ◽  
S. Lévêque-Fort ◽  
J. Siegel ◽  
M. J. Cole ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Time Domain ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Optical Sectioning ◽  
Lifetime Imaging
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