scholarly journals Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope

2012 ◽  
Vol 20 (15) ◽  
pp. 16195 ◽  
Author(s):  
Yusuke Oshima ◽  
Hidetoshi Sato ◽  
Hiroko Kajiura-Kobayashi ◽  
Tetsuaki Kimura ◽  
Kiyoshi Naruse ◽  
...  
Keyword(s):  
Light Sheet ◽  
Raman Imaging ◽  
Wide Field ◽  
Optical Sectioning

scholarly journals Challenges in 3D Live Cell Imaging

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 275
Author(s):  
Herbert Schneckenburger ◽  
Verena Richter
Keyword(s):  
Live Cell Imaging ◽  
Laser Scanning ◽  
Cell Imaging ◽  
Live Cell ◽  
Laser Scanning Microscopy ◽  
Radiative Energy ◽  
Wide Field ◽  
Optical Sectioning ◽  
Continuous Increase ◽  
Radiative Energy Transfer

A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET).

scholarly journals Using Stage- and Slit-Scanning to Improve Contrast and Optical Sectioning in Dual-View Inverted Light Sheet Microscopy (diSPIM)

2016 ◽  
Vol 231 (1) ◽  
pp. 26-39 ◽  
Author(s):  
Abhishek Kumar ◽  
Ryan Christensen ◽  
Min Guo ◽  
Panos Chandris ◽  
William Duncan ◽  
...  
Keyword(s):  
Light Sheet ◽  
Optical Sectioning ◽  
Light Sheet Microscopy ◽  
Slit Scanning

A practical wide-field Raman imaging method with high spectral and spatial resolution

2018 ◽  
Vol 89 (8) ◽  
pp. 083103 ◽  
Author(s):  
Haibo Li ◽  
Wenhua Luo ◽  
Gan Li ◽  
Guangfeng Zhang ◽  
Pengcheng Zhang ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Raman Imaging ◽  
Imaging Method ◽  
Wide Field

Optical Sectioning Fluorescence Spectroscopy in a Programmable Array Microscope

1998 ◽  
Vol 52 (6) ◽  
pp. 783-789 ◽  
Author(s):  
Quentin S. Hanley ◽  
Peter J. Verveer ◽  
Thomas M. Jovin
Keyword(s):  
Fluorescence Spectroscopy ◽  
Spatial Light Modulator ◽  
Fluorescence Emission ◽  
Region Of Interest ◽  
Wide Field ◽  
Optical Sectioning ◽  
Light Modulator ◽  
Full Field ◽  
Spectrally Resolved ◽  
Imaging Spectrograph

We report the use of a programmable array microscope (PAM) for the acquisition of spectrally resolved and high-throughput optical sections. The microscope is based on the use of a spatial light modulator for defining patterns of excitation and/or detection of fluorescence. For obtaining optically sectioned spectral images, the entrance slit of an imaging spectrograph and a line illumination pattern defined with a spatial light modulator are placed in conjugate optical positions. Compared to wide-field illumination, optical sectioning led to greater than 3× improvement in the rejection of out-of-focus fluorescence emission and nearly 6× greater peak-to-background ratios in biological specimens, yielding better contrast and spectral characterization. These effects resulted from a reduction in the artifacts arising from spectral contributions of structures outside the region of interest. We used the programmable illumination capability of the spectroscopic system to explore a variety of excitation/detection patterns for increasing the throughput of optical sectioning microscopes. A Sylvester-type Hadamard construction was particularly efficient, performing optical sectioning while maintaining a 50% optical throughput. These results demonstrate the feasibility of full-field highly multiplexed confocal spectral imaging.

scholarly journals Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser

2014 ◽  
Vol 5 (10) ◽  
pp. 3311 ◽  
Author(s):  
Atsushi Maruyama ◽  
Yusuke Oshima ◽  
Hiroko Kajiura-Kobayashi ◽  
Shigenori Nonaka ◽  
Takeshi Imamura ◽  
...  
Keyword(s):  
Pulse Energy ◽  
Light Sheet ◽  
Intravital Imaging ◽  
Wide Field ◽  
High Pulse Energy ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
High Pulse ◽  
Energy Laser

scholarly journals Simultaneous multiview capture and fusion improves spatial resolution in wide-field and light-sheet microscopy

Optica ◽  
2016 ◽  
Vol 3 (8) ◽  
pp. 897 ◽  
Author(s):  
Yicong Wu ◽  
Panagiotis Chandris ◽  
Peter W. Winter ◽  
Edward Y. Kim ◽  
Valentin Jaumouillé ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Light Sheet ◽  
Wide Field ◽  
Light Sheet Microscopy

scholarly journals Virtual-'Light-Sheet' Single-Molecule Localisation Microscopy Enables Quantitative Optical Sectioning for Super-Resolution Imaging

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0125438 ◽  
Author(s):  
Matthieu Palayret ◽  
Helen Armes ◽  
Srinjan Basu ◽  
Adam T. Watson ◽  
Alex Herbert ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Light Sheet ◽  
Optical Sectioning ◽  
Resolution Imaging ◽  
Localisation Microscopy

scholarly journals Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy

2013 ◽  
Vol 21 (9) ◽  
pp. 11425 ◽  
Author(s):  
Florian O. Fahrbach ◽  
Vasily Gurchenkov ◽  
Kevin Alessandri ◽  
Pierre Nassoy ◽  
Alexander Rohrbach
Keyword(s):  
Light Sheet ◽  
Optical Sectioning ◽  
Bessel Beams ◽  
Light Sheet Microscopy

Raman Imaging of Heterogeneous Polymers: A Comparison of Global versus Point Illumination

1995 ◽  
Vol 49 (10) ◽  
pp. 1411-1430 ◽  
Author(s):  
Lars Markwort ◽  
Bert Kip ◽  
Edouard Da Silva ◽  
Bernard Roussel
Keyword(s):  
Global Illumination ◽  
Depth Resolution ◽  
Raman Imaging ◽  
Alternative Methods ◽  
Wide Field ◽  
Light Collection ◽  
Polymer Systems ◽  
Heterogeneous Polymer ◽  
Scattering Effects ◽  
Heterogeneous Polymers

Two alternative methods of Raman imaging, via global (wide-field) illumination and via point illumination in combination with confocal light collection, have been applied to the study of heterogeneous polymer systems. From the results obtained it becomes apparent that the fluorescence inherent to most polymer systems severely limits the use of global illumination. Furthermore, the lack in depth resolution in Raman imaging by global illumination ruled out this method for the study of bulk polymer samples. Also as a consequence of the absence of depth resolution, the global illumination technique appeared more vulnerable to artifacts arising from scattering effects due to the sample geometry and fluorescence. Hence, for a general application of Raman imaging to the study of polymer samples, Raman imaging by point illumination in conjunction with confocal light collection is the method of choice

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