scholarly journals Photoacoustic imaging of lacZ gene expression in vivo

2007 ◽  
Vol 12 (2) ◽  
pp. 020504 ◽  
Author(s):  
Li Li ◽  
Roger J. Zemp ◽  
Gina Lungu ◽  
George Stoica ◽  
Lihong V. Wang
Keyword(s):  
Gene Expression ◽  
Photoacoustic Imaging ◽  
Lacz Gene

In Vivo Multispectral Photoacoustic Imaging of Gene Expression using Engineered Reporters

2015 ◽  
Author(s):  
Roger J. Zemp ◽  
Robert Paproski ◽  
A. Forbrich ◽  
Yan Li ◽  
Robert Campbell
Keyword(s):  
Gene Expression ◽  
Photoacoustic Imaging

scholarly journals Targeting the Hprt locus in mice reveals differential regulation of Tie2 gene expression in the endothelium

2000 ◽  
Vol 2 (2) ◽  
pp. 67-75 ◽  
Author(s):  
VALERIE EVANS ◽  
ANTONIS HATZOPOULOS ◽  
WILLIAM C. AIRD ◽  
HELEN B. RAYBURN ◽  
ROBERT D. ROSENBERG ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cell ◽  
Dna Sequences ◽  
Single Copy ◽  
Differential Regulation ◽  
Lacz Gene ◽  
Specific Expression ◽  
Regulatory Dna Sequences ◽  
Hprt Locus

Evans, Valerie, Antonis Hatzopoulos, William C. Aird, Helen B. Rayburn, Robert D. Rosenberg, and Jan Albert Kuivenhoven. Targeting the Hprt locus in mice reveals differential regulation of Tie2 gene expression in the endothelium. Physiol Genomics 2: 67–75, 2000.—To study the in vivo expression of the murine Tie2 gene, we have targeted the hypoxanthine phosphoribosyltransferase ( Hprt) gene locus to generate two single-copy transgenic mice: T1, containing the 2,100-bp Tie2 promoter upstream from the β-galactosidase ( LacZ) gene, and T5, which also included an enhancing element originating from the first intron of the Tie2 gene. Comparing T1 and T5 embryos at day E10.5 revealed differential endothelial cell-specific expression of LacZ, whereas colocalization analyses showed that the expression was confined to endothelial cells. Moderate reporter gene activity was observed in the brain and kidney of T1 adults, whereas extensive LacZ gene expression was seen in the vasculature of most organs of the T5 adults. This study demonstrates the feasibility of targeting the Hprt locus with endothelial cell-specific sequences to analyze the spatial-temporal expression of transgenes. Of particular importance is the observation that the analysis of a single transgene copy in a defined locus allows for an accurate and rapid comparison of transcriptional activity among regulatory DNA sequences.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

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