19 F‐NMR detection of lacZ gene expression via the enzymic hydrolysis of 2‐fluoro‐4‐nitrophenyl β‐D‐galactopyranoside in vivo in PC3 prostate tumor xenografts in the mouse 1

2007 ◽  
Vol 21 (9) ◽  
pp. 2014-2019 ◽  
Author(s):  
Li Liu ◽  
Vikram D. Kodibagkar ◽  
Jian-Xin Yu ◽  
Ralph P. Mason
Keyword(s):  
Gene Expression ◽  
Prostate Tumor ◽  
Lacz Gene ◽  
Enzymic Hydrolysis ◽  
Tumor Xenografts ◽  
Hydrolysis Of ◽  
19 F Nmr

scholarly journals MINDIN secretion by prostate tumors induces premetastatic changes in bone via β-catenin

2020 ◽  
Vol 27 (7) ◽  
pp. 441-456
Author(s):  
Juan A Ardura ◽  
Luis Álvarez-Carrión ◽  
Irene Gutiérrez-Rojas ◽  
Peter A Friedman ◽  
Arancha R Gortázar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Tumor Cell ◽  
Ex Vivo ◽  
Prostate Tumor ◽  
Tumor Cell Adhesion ◽  
Prostate Tumors ◽  
Cell Homing

Bone metastases are common in advanced prostate cancer patients, but mechanisms by which specific pro-metastatic skeletal niches are formed before tumor cell homing are unclear. We aimed to analyze the effects of proteins secreted by primary prostate tumors on the bone microenvironment before the settlement and propagation of metastases. Here, using an in vivo pre-metastatic prostate cancer model based on the implantation of prostate adenocarcinoma TRAMP-C1 cells in immunocompetent C57BL/6 mice, we identify MINDIN as a prostate tumor secreted protein that induces bone microstructural and bone remodeling gene expression changes before tumor cell homing. Associated with these changes, increased tumor cell adhesion to the endosteum ex vivo and to osteoblasts in vitro was observed. Furthermore, MINDIN promoted osteoblast proliferation and mineralization and monocyte expression of osteoclast markers. β-catenin signaling pathway revealed to mediate MINDIN actions on osteoblast gene expression but failed to affect MINDIN-induced adhesion to prostate tumor cells or monocyte differentiation to osteoclasts. Our study evidences that MINDIN secretion by primary prostate tumors creates a favorable bone environment for tumor cell homing before metastatic spread.

Enzymic Hydrolysis of Stilbestrol Diphosphate in vitro and in vivo.

1961 ◽  
Vol 106 (2) ◽  
pp. 327-330 ◽  
Author(s):  
W. Johnson ◽  
R. Jasmin ◽  
G. Corte
Keyword(s):  
Enzymic Hydrolysis ◽  
Hydrolysis Of

scholarly journals SIMILARITY OF THE KINETICS OF INVERTASE ACTION IN VIVO AND IN VITRO

1932 ◽  
Vol 15 (5) ◽  
pp. 491-495 ◽  
Author(s):  
J. M. Nelson ◽  
Elizabeth T. Palmer ◽  
B. G. Wilkes
Keyword(s):  
Yeast Cells ◽  
Enzymic Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Live Yeast

1. A method is given whereby the course of hydrolysis of sucrose by live yeast cells may be followed with precision equal to that found when invertase solutions prepared from autolyzed yeast are used to cause inversion. 2. The practical value of the equation of Nelson and Hitchcock as a means of following the course of enzymic hydrolysis of sucrose is hereby extended. 3. The inversion of sucrose by live yeast cells and by extracted invertase has been quantitatively compared. 4. The course of hydrolysis of sucrose by the invertase of Fleischmann's yeast has been found to be identical in vivo and in vitro.

scholarly journals Clinically Relevant Doses of Candesartan Inhibit Growth of Prostate Tumor Xenografts In Vivo through Modulation of Tumor Angiogenesis

2014 ◽  
Vol 350 (3) ◽  
pp. 635-645 ◽  
Author(s):  
Ahmed Alhusban ◽  
Ahmad Al-Azayzih ◽  
Anna Goc ◽  
Fei Gao ◽  
Susan C. Fagan ◽  
...  
Keyword(s):  
Tumor Angiogenesis ◽  
Prostate Tumor ◽  
Tumor Xenografts

scholarly journals Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo.

1975 ◽  
Vol 67 (2) ◽  
pp. 484-488 ◽  
Author(s):  
D K Myers ◽  
L E Feinendegen
Keyword(s):  
T Cells ◽  
Human Kidney ◽  
Enzymic Hydrolysis ◽  
Double Labeling ◽  
Exogenous Dna ◽  
Mouse Thymus ◽  
Whole Cells ◽  
Thymus Cells ◽  
Hydrolysis Of

Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.

scholarly journals Photoacoustic imaging of lacZ gene expression in vivo

2007 ◽  
Vol 12 (2) ◽  
pp. 020504 ◽  
Author(s):  
Li Li ◽  
Roger J. Zemp ◽  
Gina Lungu ◽  
George Stoica ◽  
Lihong V. Wang
Keyword(s):  
Gene Expression ◽  
Photoacoustic Imaging ◽  
Lacz Gene
Sign in / Sign up

Export Citation Format

Share Document