Photoacoustic imaging of fear conditioning neuronal ensembles using a Fos-Lacz gene reporter system in the rat brain in vivo after intrathecal X-gal injection

2021 ◽  
Author(s):  
James Matchynski ◽  
Rayyan Manwar ◽  
Karl Kratkiewicz ◽  
Rajtarun Madangopal ◽  
Veronica A. Lennon ◽  
...  
Keyword(s):  
Rat Brain ◽  
Fear Conditioning ◽  
Photoacoustic Imaging ◽  
Lacz Gene ◽  
Reporter System ◽  
Neuronal Ensembles

scholarly journals Hippocampus and amygdala fear memory engrams re-emerge after contextual fear reinstatement

2019 ◽  
Author(s):  
Yosif Zaki ◽  
William Mau ◽  
Christine Cincotta ◽  
Anahita Hamidi ◽  
Emily Doucette ◽  
...  
Keyword(s):  
Fear Conditioning ◽  
Basolateral Amygdala ◽  
Fear Memory ◽  
Neural Substrates ◽  
Memory Representation ◽  
Contextual Fear ◽  
Neuronal Ensembles ◽  
State Present ◽  
Insight Into

AbstractThe formation and extinction of fear memories represent two forms of learning that each engage the hippocampus and amygdala. How cell populations in these areas contribute to fear relapse, however, remains unclear. Here, we demonstrate that, in mice, cells active during fear conditioning in the dentate gyrus of hippocampus and basolateral amygdala exhibit decreased activity during extinction and are re-engaged after fear reinstatement. In vivo calcium imaging reveals that reinstatement drives population dynamics in the basolateral amygdala to revert to a network state similar to the state present during fear conditioning. Finally, we find that optogenetic inactivation of neuronal ensembles active during fear conditioning in either the hippocampus or amygdala is sufficient to disrupt fear expression after reinstatement. These results suggest that fear reinstatement triggers a partial re-emergence of the original fear memory representation, providing new insight into the neural substrates of fear relapse.

scholarly journals A human photoacoustic imaging reporter gene using the clinical dye indocyanine green

2019 ◽  
Author(s):  
Nivin N. Nyström ◽  
Lawrence C.M. Yip ◽  
Jeffrey J.L. Carson ◽  
Timothy J. Scholl ◽  
John A. Ronald
Keyword(s):  
Indocyanine Green ◽  
Cell Tracking ◽  
Photoacoustic Imaging ◽  
Organic Anion ◽  
Reporter System ◽  
Optical Contrast ◽  
Organic Anion Transporting Polypeptide ◽  
Gene Therapies

ABSTRACTPhotoacoustic imaging (PAI) combines optical contrast with the resolution and depth-detection of ultrasound and is increasingly being utilized for medical imaging in patients. PAI reporter genes would allow for monitoring of cell and gene therapies, but current reporters have immunogenicity and/or toxicity concerns that may limit clinical translation. Here we report a PAI reporter system employing the ability of human organic anion transporting polypeptide 1b3 (Oatp1b3) to take up the clinical dye indocyanine green (ICG) into cells. Following ICG administration, cells synthetically expressing Oatp1b3 exhibited significantly increased PAI signals compared to control cells both in vitro and in mice. Several benefits of this technology are the human derivation of Oatp1b3, and the high extinction coefficient, low quantum yield and pre-existing clinical approval of ICG. We posit that the Oatp1b3-ICG reporter system could be useful for in vivo gene and cell tracking in preclinical and clinical applications.

A UCP1 Near-Infrared Reporter System for Monitoring Adipose “Browning” In Vivo Using Photoacoustic Imaging

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 280-OR
Author(s):  
DERRYN XIN HUI CHAN ◽  
SHIGEKI SUGII ◽  
WEIPING HAN
Keyword(s):  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Reporter System

scholarly journals Photoacoustic imaging of lacZ gene expression in vivo

2007 ◽  
Vol 12 (2) ◽  
pp. 020504 ◽  
Author(s):  
Li Li ◽  
Roger J. Zemp ◽  
Gina Lungu ◽  
George Stoica ◽  
Lihong V. Wang
Keyword(s):  
Gene Expression ◽  
Photoacoustic Imaging ◽  
Lacz Gene

Sigma receptors mediate potent neuroprotection in vivo and inhibit neuronal depolarisation and swelling in rat brain slices

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S468-S468
Author(s):  
Jennifer K Callaway ◽  
Christine Molnar ◽  
Song T Yao ◽  
Bevyn Jarrott ◽  
R David Andrew
Keyword(s):  
Rat Brain ◽  
Brain Slices ◽  
Sigma Receptors

In vivo diffusion tensor imaging of the neonatal rat brain development

2013 ◽  
Vol 44 (S 01) ◽  
Author(s):  
M Breu ◽  
D Reisinger ◽  
D Wu ◽  
Y Zhang ◽  
A Fatemi ◽  
...  
Keyword(s):  
Diffusion Tensor Imaging ◽  
Brain Development ◽  
Rat Brain ◽  
Diffusion Tensor ◽  
Neonatal Rat ◽  
Neonatal Rat Brain

scholarly journals Studies on Aromatic Amino Acid Uptake by Rat Brain in Vivo

1962 ◽  
Vol 237 (3) ◽  
pp. 803-806
Author(s):  
Gordon Guroff ◽  
Sidney Udenfriend
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Aromatic Amino Acid ◽  
Amino Acid Uptake ◽  
Acid Uptake

In Vivo Detection of Redox-Inactive Neurochemicals in the Rat Brain with an Ion Transfer Microsensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Xiaofang Wang ◽  
Tianci Xu ◽  
Yue Zhang ◽  
Nan Gao ◽  
Taotao Feng ◽  
...  
Keyword(s):  
Rat Brain ◽  
Ion Transfer ◽  
In Vivo Detection
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