Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture

2003 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Rebekah Drezek ◽  
Martial Guillaud ◽  
Thomas Collier ◽  
Iouri Boiko ◽  
Anais Malpica ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dna Content ◽  
Nuclear Morphology ◽  
Neoplastic Progression ◽  
Chromatin Texture ◽  
Cervical Cells

scholarly journals DNA Ploidy and Markovian Analysis of Neoplastic Progression in Experimental Pancreatic Cancer

2003 ◽  
Vol 51 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Russell G. Postier ◽  
Megan R. Lerner ◽  
Stan A. Lightfoot ◽  
Rick Vannarath ◽  
Mary M. Lane ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Pancreatic Adenocarcinoma ◽  
Dna Ploidy ◽  
Computer Assisted ◽  
Nuclear Morphology ◽  
Neoplastic Progression ◽  
Atypical Cell ◽  
Atypical Cells ◽  
Experimental Pancreatic Cancer ◽  
Markovian Analysis

Computer-assisted analysis of DNA ploidy and nuclear morphology were used to elucidate changes in the cell nucleus that occur during the development of experimental pancreatic cancer. Ductal pancreatic adenocarcinoma was induced in 49 Syrian hamsters by SC injection of N-nitrosobis (2-oxopropyl) amine; twenty hamsters served as controls. Groups of animals were sacrificed every 4 weeks for 20 weeks and adjacent sections of pancreatic tissue were H&E and Feulgen-stained for light microscopy and computer assisted cytometry. Pancreatic ductal cells were classified as normal, atypical, or malignant; tissue inflammation (pancreatitis) was also noted when present. DNA ploidy and nuclear morphology evaluation (Markovian analysis) identified an atypical cell stage clearly distinguishable from either normal or malignant cells; pancreatitis preceded this atypia. The DNA ploidy histogram of these atypical cells revealed a major diploid peak and a minor aneuploid peak. The receiver operator characteristic curve areas for a logistic regression model of normal vs atypical cells was 0.94 and for atypical vs malignant was 0.98, numbers indicative of near-perfect discrimination among these three cell types. The ability to identify an atypical cell population should be useful in establishing the role of these cells in the progression of human pancreatic adenocarcinoma.

scholarly journals Automated imaging cytometry reveals dysplastic indices of colonic serrated adenomas

2020 ◽  
pp. FSO459
Author(s):  
Nicholas S Samel ◽  
Qin Huang ◽  
Hiroshi Mashimo
Keyword(s):  
Dna Content ◽  
Chromosome Instability ◽  
Neoplastic Progression ◽  
Hyperplastic Polyps ◽  
Dna Index ◽  
Tissue Sections ◽  
Automated Imaging ◽  
Imaging Cytometry ◽  
The Mean ◽  
Serrated Adenomas

Aim: Left-sided colonic serrated adenomas (L-SAs) were evaluated for aneuploidy using automated imaging cytometry to quantify DNA content and compared with normal colonic tissues (NCT), tubular adenomas (TA), left-sided hyperplastic polyps (L-HP) and adenocarcinomas. Materials & methods: We used standard paraffin-embedded Feulgen-stained tissue sections. Results: The mean DNA index (DI) of NCT was 0.95, L-HP was 1.08, TA was 1.22, L-SA was 1.11 and adenocarcinomas was 1.46. DI of L-SA was statistically higher than that of NCT, but not statistically different from L-HP. Conclusion: This study demonstrates that DIs correlate with the described neoplastic progression of L-SA, TA and L-SA compared with NCT and suggests that L-SA may be involved in a chromosome instability pathway of neoplastic progression.

Changes in nuclear morphology and chromatin texture of basal keratinocytes in melasma

2014 ◽  
Vol 29 (4) ◽  
pp. 809-812 ◽  
Author(s):  
G. Brianezi ◽  
A.C. Handel ◽  
J.V. Schmitt ◽  
L.D.B. Miot ◽  
H.A. Miot
Keyword(s):  
Nuclear Morphology ◽  
Basal Keratinocytes ◽  
Chromatin Texture

Nuclear morphology and DNA content in the glandular trichomes of peppermint (Mentha×piperita L.)

PROTOPLASMA ◽  
1993 ◽  
Vol 175 (3-4) ◽  
pp. 85-92 ◽  
Author(s):  
Graziella Berta ◽  
M. Dela Pierre ◽  
M. Maffei
Keyword(s):  
Dna Content ◽  
Glandular Trichomes ◽  
Mentha Piperita ◽  
Nuclear Morphology

scholarly journals IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248639
Author(s):  
Camila Mareti Bonin-Jacob ◽  
Larissa Zatorre Almeida-Lugo ◽  
Marco Antonio Moreira Puga ◽  
Ana Paula Machado ◽  
Cacilda Tezelli Junqueira Padovani ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Uterine Cervix ◽  
Hpv Infection ◽  
Single Infection ◽  
Multiple Infections ◽  
Neoplastic Progression ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Cells

Persistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-4, and IL-2 were quantified in the serum and exfoliated cervical cells (ECCs) of patients with HR-HPV, and the presence of IL-6+ cells was investigated in uterine cervix biopsies. Cytokine levels in the serum and ECCs of 26 HR-HPV DNA-positive patients and 18 HPV DNA-negative patients were measured using flow cytometry. Fifteen uterine cervix biopsy samples embedded in paraffin were subjected to immunohistochemical analysis for the detection of IL-6+ cells. HR-HPV-positive patients showed increased IL-6 and IL-10 in the ECCs and serum, respectively. Compared with HPV DNA-positive patients, HPV DNA-negative patients had higher levels of IL-6 in ECCs. Patients with multiple infections of HPV had higher levels of IL-6 in their ECCs than those with a single infection. Immunostaining of uterine cervix biopsy samples revealed no differences in IL-6 expression between the different classes of histopathological lesions. However, differences were observed in the expression levels of IL-6 and IL-10 at the systemic and local levels in HR-HPV-positive patients without cervical lesions. Considering the functional characteristics of these cytokines, it can be inferred that such patients are prone to persistent HPV infection.

Sign in / Sign up

Export Citation Format

Share Document