scholarly journals DNA content and chromatin texture of human breast epithelial cells transformed with 17-β-estradiol and the estrogen antagonist ICI 182,780 as assessed by image analysis

2007 ◽  
Vol 617 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Maria Luiza S. Mello ◽  
Benedicto C. Vidal ◽  
Irma H. Russo ◽  
Mohamed H. Lareef ◽  
Jose Russo
Keyword(s):  
Image Analysis ◽  
Epithelial Cells ◽  
Dna Content ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Ici 182,780 ◽  
Chromatin Texture ◽  
Breast Epithelial ◽  
Estrogen Antagonist ◽  
Human Breast Epithelial Cells

scholarly journals Ha-rasOncogene Effect on DNA Content and Chromatin Supraorganization in benzo[a]pyrene-Transformed Human Breast Epithelial Cells

1999 ◽  
Vol 19 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Maria Luiza S. Mello ◽  
Benedicto de Campos Vidal ◽  
Jose Russo
Keyword(s):  
Image Analysis ◽  
Epithelial Cells ◽  
Cell Line ◽  
Dna Content ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells ◽  
Tumorigenic Cell Line ◽  
Tumorigenic Cell

When transfected to benzo[a]pyrene (BP)‐transformed MCF‐10F human breast epithelial cells (BP1 cell line) the c‐Ha‐rasoncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1‐Tras. We have previously demonstrated by image analysis that BP affects the DNA content and the chromatin supraorganization of MCF‐10F cells. Here Feulgen‐stained BP1‐Trascells were studied by image analysis in order to evaluate possible additional changes in DNA content and chromatin texture induced by insertion of therasoncogene. A high variability in DNA content also including polyploidy or near‐polyploidy, and an increase in the packing states of the chromatin which became still condensed in BP1 cells were found in BP1‐Trascells. The results differed from those reported for the BP1‐E1 cell line which is also an aggressive tumorigenic cell line, but was attained through progressive passages of BP‐transformed cells. It was demonstrated that different patterns of changes in DNA content and chromatin organization may be involved in equally aggressive tumorigenic BP‐transformed cell lines originated from the same cell line by different mechanisms.

scholarly journals Image analysis of the AgNOR response in ras-transformed human breast epithelial cells

2008 ◽  
Vol 110 (3) ◽  
pp. 210-216 ◽  
Author(s):  
Maria Luiza S. Mello ◽  
Benedicto C. Vidal ◽  
Jose Russo ◽  
Wolfgang Planding ◽  
Ulrich Schenck
Keyword(s):  
Image Analysis ◽  
Epithelial Cells ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells

scholarly journals Oncogenic p95HER2/611CTF primes human breast epithelial cells for metabolic stress-induced down-regulation of FLIP and activation of TRAIL-R/Caspase-8-dependent apoptosis

Oncotarget ◽  
2017 ◽  
Vol 8 (55) ◽  
pp. 93688-93703 ◽  
Author(s):  
Rosa Martín-Pérez ◽  
Rosario Yerbes ◽  
Rocío Mora-Molina ◽  
Ana Cano-González ◽  
Joaquín Arribas ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Metabolic Stress ◽  
Caspase 8 ◽  
Human Breast ◽  
Down Regulation ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells
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