AFM Studies of Solid-Supported Lipid Bilayers Formed at a Au(111) Electrode Surface Using Vesicle Fusion and a Combination of Langmuir−Blodgett and Langmuir−Schaefer Techniques

Langmuir ◽  
2008 ◽  
Vol 24 (18) ◽  
pp. 10313-10323 ◽  
Author(s):  
Ming Li ◽  
Maohui Chen ◽  
Erin Sheepwash ◽  
Christa L. Brosseau ◽  
Hongqiang Li ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Electrode Surface ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
Langmuir Blodgett

scholarly journals Preparation and Characterization of Solid-Supported Lipid Bilayers Formed by Langmuir–Blodgett Deposition: A Tutorial

Langmuir ◽  
2018 ◽  
Vol 34 (51) ◽  
pp. 15622-15639 ◽  
Author(s):  
James Kurniawan ◽  
João Francisco Ventrici de Souza ◽  
Amanda T. Dang ◽  
Gang-yu Liu ◽  
Tonya L. Kuhl
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Langmuir Blodgett

scholarly journals Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

Langmuir ◽  
2014 ◽  
Vol 30 (25) ◽  
pp. 7259-7263 ◽  
Author(s):  
Tania Kjellerup Lind ◽  
Marité Cárdenas ◽  
Hanna Pauliina Wacklin
Keyword(s):  
Lipid Bilayers ◽  
Deposition Temperature ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers

scholarly journals Biomimetic supported lipid bilayers with high cholesterol content formed by α-helical peptide-induced vesicle fusion

2012 ◽  
Vol 22 (37) ◽  
pp. 19506 ◽  
Author(s):  
Gregory J. Hardy ◽  
Rahul Nayak ◽  
S. Munir Alam ◽  
Joseph G. Shapter ◽  
Frank Heinrich ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Cholesterol Content ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
High Cholesterol ◽  
Helical Peptide

scholarly journals In vitro single vesicle fusion assays based on pore-spanning membranes: merits and drawbacks

2020 ◽  
Author(s):  
Peter Mühlenbrock ◽  
Merve Sari ◽  
Claudia Steinem
Keyword(s):  
Lipid Bilayers ◽  
Vesicle Fusion ◽  
In Vitro Assays ◽  
Supported Lipid Bilayers ◽  
Protein Receptors ◽  
Membrane Components ◽  
Interconnected Structure ◽  
Single Vesicle ◽  
Fusion Efficiency

AbstractNeuronal fusion mediated by soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNAREs) is a fundamental cellular process by which two initially distinct membranes merge resulting in one interconnected structure to release neurotransmitters into the presynaptic cleft. To get access to the different stages of the fusion process, several in vitro assays have been developed. In this review, we provide a short overview of the current in vitro single vesicle fusion assays. Among those assays, we developed a single vesicle assay based on pore-spanning membranes (PSMs) on micrometre-sized pores in silicon, which might overcome some of the drawbacks associated with the other membrane architectures used for investigating fusion processes. Prepared by spreading of giant unilamellar vesicles with reconstituted t-SNAREs, PSMs provide an alternative tool to supported lipid bilayers to measure single vesicle fusion events by means of fluorescence microscopy. Here, we discuss the diffusive behaviour of the reconstituted membrane components as well as that of the fusing synthetic vesicles with reconstituted synaptobrevin 2 (v-SNARE). We compare our results with those obtained if the synthetic vesicles are replaced by natural chromaffin granules under otherwise identical conditions. The fusion efficiency as well as the different fusion states observable in this assay by means of both lipid mixing and content release are illuminated.

scholarly journals Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

2008 ◽  
Vol 3 (2) ◽  
pp. FA108-FA116 ◽  
Author(s):  
Fredrik Höök ◽  
Gudrun Stengel ◽  
Andreas B. Dahlin ◽  
Anders Gunnarsson ◽  
Magnus P. Jonsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
Microscopy Techniques
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