Fabrication of three-dimensional microstructures by two-dimensional slice by slice approaching via focused ion beam milling

Author(s):  
Yongqi Fu ◽  
Ngoi Kok Ann Bryan
2014 ◽  
Vol 5 ◽  
pp. 1066-1070 ◽  
Author(s):  
Andreas Landefeld ◽  
Joachim Rösler

Background: This paper describes the shaping of freestanding objects out of metallic structures in the nano- and submicron size. The technique used, called nanoforging, is very similar to the macroscopic forging process. Results: With spring actuated tools produced by focused ion beam milling, controlled forging is demonstrated. With only three steps, a conical bar stock is transformed to a flat- and semicircular bent bar stock. Conclusion: Compared with other forming techniques in the reduced scale, nanoforging represents a beneficial approach in forming freestanding metallic structures, due to its simplicity, and supplements other forming techniques.


2006 ◽  
Author(s):  
Wico C. L. Hopman ◽  
René M. de Ridder ◽  
Shankar Selvaraja ◽  
Cazimir G. Bostan ◽  
Vishwas J. Gadgil ◽  
...  

1990 ◽  
Vol 137 (3) ◽  
pp. 983-988 ◽  
Author(s):  
Fumikazu Itoh ◽  
Akira Shimase ◽  
Satoshi Haraichi

2015 ◽  
Vol 25 (1) ◽  
pp. 49 ◽  
Author(s):  
Terence Wagenknecht ◽  
Chyongere Hsieh ◽  
Michael Marko

Cryo-electron tomography (cryo-ET) has emerged as perhaps the only practical technique for revealing nanometer-level three-dimensional structural details of subcellular macromolecular complexes in their native context, inside the cell. As currently practiced, the specimen should be 0.1- 0.2 microns in thickness to achieve optimal resolution. Thus, application of cryo-ET to intact frozen (vitreous) tissues, such as skeletal muscle, requires that they be sectioned. Cryo-ultramicrotomy is notoriously difficult and artifact-prone when applied to frozen cells and tissue, but a new technique, focused ion beam milling (cryo-FIB), shows great promise for “thinning” frozen biological specimens. Here we describe our initial results in applying cryo-FIB and cryo-ET to triad junctions of skeletal muscle.


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