Hydroxyapatite Scaffold Porosity, Bone Regeneration, and Vascularization Using an Adapted CAM Assay

2012 ◽  
Author(s):  
A. J. Wagoner Johnson ◽  
S. J. Polak
Keyword(s):  
Bone Regeneration ◽  
Direct Write ◽  
Cam Assay ◽  
Implant Fixation ◽  
Manufacturing Methods ◽  
Hydroxyapatite Scaffold ◽  
Specific Influence

Hydroxyapatite (HA) is used in a number of applications for bone restoration and implant fixation. Direct-write manufacturing methods enable the fabrication of HA scaffolds with precisely controlled macroporosity (>100μm) between scaffold struts, or rods, and microporosity (<20μm) within the rods [1]. The need for macroporosity for bone regeneration is well documented, though its specific influence on directing vascularization is not as well studied. Limited research has investigated the role of microporosity in bone regeneration or vascularization. This work aims to address these gaps.

scholarly journals Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1152
Author(s):  
Alberto Polo-Montalvo ◽  
Laura Casarrubios ◽  
María Concepción Serrano ◽  
Adrián Sanvicente ◽  
María José Feito ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mesoporous Structure ◽  
Ion Release ◽  
Endothelial Progenitor ◽  
Matrix Mineralization ◽  
Intracellular Content

Due to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processes, depending on their phenotype. In this work, the action of ion release from MBG-75S, with a molar composition of 75SiO2-20CaO-5P2O5, on osteogenesis and angiogenesis and the modulatory role of macrophages have been assessed in vitro with MC3T3-E1 pre-osteoblasts and endothelial progenitor cells (EPCs) in monoculture and in coculture with RAW 264.7 macrophages. Ca2+, phosphorous, and silicon ions released from MBG-75S were measured in the culture medium during both differentiation processes. Alkaline phosphatase activity and matrix mineralization were quantified as the key markers of osteogenic differentiation in MC3T3-E1 cells. The expression of CD31, CD34, VEGFR2, eNOS, and vWF was evaluated to characterize the EPC differentiation into mature endothelial cells. Other cellular parameters analyzed included the cell size and complexity, intracellular calcium, and intracellular content of the reactive oxygen species. The results obtained indicate that the ions released by MBG-75S promote osteogenesis and angiogenesis in vitro, evidencing a macrophage inhibitory role in these processes and demonstrating the high potential of MBG-75S for the preparation of implants for bone regeneration.

scholarly journals Involvement of the long noncoding RNA H19 in osteogenic differentiation and bone regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zimo Zhou ◽  
Mohammad Showkat Hossain ◽  
Da Liu
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Osteoblast Differentiation ◽  
Osteoclast Differentiation ◽  
Complex Processes ◽  
Osteogenic Induction ◽  
Novel Target

AbstractOsteogenic differentiation and bone regeneration are complex processes involving multiple genes and multiple steps. In this review, we summarize the effects of the long noncoding RNA (lncRNA) H19 on osteogenic differentiation.Osteogenic differentiation includes matrix secretion and calcium mineralization as hallmarks of osteoblast differentiation and the absorption of calcium and phosphorus as hallmarks of osteoclast differentiation. Mesenchymal stem cells (MSCs) form osteoprogenitor cells, pre-osteoblasts, mature osteoblasts, and osteocytes through induction and differentiation. lncRNAs regulate the expression of coding genes and play essential roles in osteogenic differentiation and bone regeneration. The lncRNA H19 is known to have vital roles in osteogenic induction.This review highlights the role of H19 as a novel target for osteogenic differentiation and the promotion of bone regeneration.

scholarly journals Delivery Systems and Role of Growth Factors for Alveolar Bone Regeneration in Dentistry

10.5772/55580 ◽  
2013 ◽  
Author(s):  
Stefano Sivolella ◽  
Marleen De ◽  
Giulia Brunello ◽  
Sara Ricci ◽  
Drazen Tadic ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Regeneration ◽  
Alveolar Bone ◽  
Delivery Systems

Role of Discoidin Domain Receptor 2 in Craniofacial Bone Regeneration

2021 ◽  
pp. 002203452110074
Author(s):  
A. Binrayes ◽  
C. Ge ◽  
F.F. Mohamed ◽  
R.T. Franceschi
Keyword(s):  
Bone Regeneration ◽  
Healing Process ◽  
Congenital Defects ◽  
Calvarial Bone ◽  
Discoidin Domain Receptor ◽  
Skeletal Defects ◽  
Discoidin Domain Receptor 2 ◽  
Collagen Receptor ◽  
Deficient Mice

Bone loss caused by trauma, neoplasia, congenital defects, or periodontal disease is a major cause of disability and human suffering. Skeletal progenitor cell–extracellular matrix interactions are critical for bone regeneration. Discoidin domain receptor 2 (DDR2), an understudied collagen receptor, plays an important role in skeletal development. Ddr2 loss-of-function mutations in humans and mice cause severe craniofacial and skeletal defects, including altered cranial shape, dwarfing, reduced trabecular and cortical bone, alveolar bone/periodontal defects, and altered dentition. However, the role of this collagen receptor in craniofacial regeneration has not been examined. To address this, calvarial subcritical-size defects were generated in wild-type (WT) and Ddr2-deficient mice. The complete bridging seen in WT controls at 4 wk postsurgery was not observed in Ddr2-deficient mice even after 12 wk. Quantitation of defect bone area by micro–computed tomography also revealed a 50% reduction in new bone volume in Ddr2-deficient mice. Ddr2 expression during calvarial bone regeneration was measured using Ddr2-LacZ knock-in mice. Expression was restricted to periosteal surfaces of uninjured calvarial bone and, after injury, was detected in select regions of the defect site by 3 d postsurgery and expanded during the healing process. The impaired bone healing associated with Ddr2 deficiency may be related to reduced osteoprogenitor or osteoblast cell proliferation and differentiation since knockdown/knockout of Ddr2 in a mesenchymal cell line and primary calvarial osteoblast cultures reduced osteoblast differentiation while Ddr2 overexpression was stimulatory. In conclusion, Ddr2 is required for cranial bone regeneration and may be a novel target for therapy.

Specific influence of the media on the transformation of political values and orientations of Russian youth

2020 ◽  
pp. 8-15
Author(s):  
Elena Nikolaevna Malik
Keyword(s):  
Young People ◽  
Media Literacy ◽  
World Politics ◽  
Political Consciousness ◽  
Information Culture ◽  
Political Values ◽  
The Media ◽  
New Challenges ◽  
Specific Influence

The article reveals the role of the institute of mass media on the processes of forming political consciousness and socio-political guidelines of young citizens in modern Russia. The problems of hygiene of media policy, media literacy and improving the information culture of young people remain relevant and archival, given the new challenges of world politics and the geopolitical situation. The author argues that media education technologies to increase the media literacy of young citizens contribute to the realization of their socio-political subjectivity and initiative in the interests of the state and civil society.

scholarly journals Role of Osteogenic Growth Peptide (OGP) and OGP(10–14) in Bone Regeneration: A Review

2016 ◽  
Vol 17 (11) ◽  
pp. 1885 ◽  
Author(s):  
Suzane Pigossi ◽  
Marcell Medeiros ◽  
Sybele Saska ◽  
Joni Cirelli ◽  
Raquel Scarel-Caminaga
Keyword(s):  
Bone Regeneration

scholarly journals The role of hesperetin on osteogenesis of human mesenchymal stem cells and its function in bone regeneration

Oncotarget ◽  
2017 ◽  
Vol 8 (13) ◽  
pp. 21031-21043 ◽  
Author(s):  
Deting Xue ◽  
Erman Chen ◽  
Wei Zhang ◽  
Xiang Gao ◽  
Shengdong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Human Mesenchymal Stem Cells

The Role of Bone Decortication in Enhancing the Results of Guided Bone Regeneration: A Literature Review

2009 ◽  
Vol 80 (2) ◽  
pp. 175-189 ◽  
Author(s):  
G. Greenstein ◽  
B. Greenstein ◽  
J. Cavallaro ◽  
D. Tarnow
Keyword(s):  
Literature Review ◽  
Bone Regeneration ◽  
Guided Bone Regeneration

scholarly journals Anabolic and antiresorptive actions of locally delivered bisphosphonates for bone repair

2018 ◽  
Vol 7 (10) ◽  
pp. 548-560 ◽  
Author(s):  
I. Qayoom ◽  
D. B. Raina ◽  
A. Širka ◽  
Š. Tarasevičius ◽  
M. Tägil ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Regeneration ◽  
Bone Morphogenetic Proteins ◽  
Bone Repair ◽  
Biological Effects ◽  
Research Groups ◽  
Preclinical Models ◽  
Implant Fixation ◽  
Local Use

During the last decades, several research groups have used bisphosphonates for local application to counteract secondary bone resorption after bone grafting, to improve implant fixation or to control bone resorption caused by bone morphogenetic proteins (BMPs). We focused on zoledronate (a bisphosphonate) due to its greater antiresorptive potential over other bisphosphonates. Recently, it has become obvious that the carrier is of importance to modulate the concentration and elution profile of the zoledronic acid locally. Incorporating one fifth of the recommended systemic dose of zoledronate with different apatite matrices and types of bone defects has been shown to enhance bone regeneration significantly in vivo. We expect the local delivery of zoledronate to overcome the limitations and side effects associated with systemic usage; however, we need to know more about the bioavailability and the biological effects. The local use of BMP-2 and zoledronate as a combination has a proven additional effect on bone regeneration. This review focuses primarily on the local use of zoledronate alone, or in combination with bone anabolic factors, in various preclinical models mimicking different orthopaedic conditions. Cite this article: I. Qayoom, D. B. Raina, A. Širka, Š. Tarasevičius, M. Tägil, A. Kumar, L. Lidgren. Anabolic and antiresorptive actions of locally delivered bisphosphonates for bone repair: A review. Bone Joint Res 2018;7:548–560. DOI: 10.1302/2046-3758.710.BJR-2018-0015.R2.

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