Analysis of Electrokinetic Fluid Flow in T-Shaped DNA Chips

2012 ◽  
Author(s):  
Yogendra M. Panta ◽  
Sanket Aryal ◽  
Param C. Adhikari
Keyword(s):  
Electric Fields ◽  
Chemical Engineering ◽  
Fluid Velocity ◽  
Entire Solution ◽  
Ionic Concentration ◽  
Buffer Solution ◽  
Lab On Chip ◽  
On Chip ◽  
Novel Applications

Lab-on-chip devices promise for many novel applications concerning the transport of the liquid samples and other solutions in the order of micro-scale dimensions. One of the efficient methods for transporting fluid in the samples is through electrokinetic effects, where an electric field will be applied to charged ions such as DNA, a negatively charged ion or proteins. These ions are carried over in the microchannel by the application of electric fields through the entire solution from inlet via probe region for its detection to outlet and the determination of concentration distribution. COMSOL, commercially available multiphysics software, with its specific MEMS and Chemical Engineering modules were employed and simulated for the analysis of fluid velocity and ionic concentration throughout the channel of various shapes. The ionic fluid concentrations and velocities in the channel and at the outlet are plotted against the potential differences across the two inlets in which DNA sample was introduced from one inlet and a buffer solution was supplied from another inlet.

Capture of Magnetic Microspheres in Electrokinetic Flow for Application in Lab-on-Chip Devices

2012 ◽  
Author(s):  
Debarun Das ◽  
Marwan Al-Rjoub ◽  
Jagjit S. Yadav ◽  
Rupak K. Banerjee
Keyword(s):  
Magnetic Field ◽  
Electric Fields ◽  
Magnetic Beads ◽  
Magnetic Microspheres ◽  
Electrokinetic Flow ◽  
Lab On Chip ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
On Chip ◽  
The Magnetic Field

Isolation of bio-molecules, cells and pathogens for immunoassays is a critical component in micro total analysis systems (μTAS). Magnetophoretic technique is often used for separation of such target species, where magnetic beads tagged with specific antibodies against cell surface epitopes, are captured in the microfluidic device. In this study, a numerical model is developed for capture of beads under an external magnetic field in electrokinetically driven flow. The results indicate an increase in the number of beads captured when the magnetic field is higher and the flow is driven by lower electric fields.

scholarly journals Determination of HMW – GS in wheat using SDS – PAGE and Lab-on-chip methods

10.5219/995 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 477-481 ◽  
Author(s):  
Timea Kuťka Hlozáková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Zdenka Gálová ◽  
Milan Chňapek ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Wheat Breeding ◽  
The Other ◽  
Gluten Protein ◽  
Gluten Proteins ◽  
Lab On Chip ◽  
Sds Page ◽  
Novel Complex ◽  
On Chip

SDS-PAGE is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoC (Lab-on-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. Benefits and limitations of Lab-on-Chip method over SDS-PAGE method in gluten proteins evaluation were explored in order to determine in which way LoC method should be improved in order to make its results more compliant with the results of SDS-PAGE. Chip electrophoresis provides a very good reproducibility of HMW-GS patterns. Moreover this approach is much faster than the conventional SDS-PAGE methods requiring several hours for an analysis. Another advantage over traditional gel electrophoresis is lower sample and reagent volume requirements, as well as specialized protein standards for accurate reproducibility and quantification. In the present study, we identified novel complex allele located at the locus Glu-1B.

Micro-PIV Measurements of Flowfields Within Plugs in Two Phase Flows for µ-TAS Applications

2006 ◽  
Author(s):  
Colin King ◽  
Edmond Walsh ◽  
Ronan Grimes
Keyword(s):  
Two Phase Flow ◽  
Fluid Velocity ◽  
Internal Diameter ◽  
Immiscible Fluid ◽  
Phase Flow ◽  
Internal Circulation ◽  
Two Phase ◽  
Lab On Chip ◽  
Micro Piv ◽  
On Chip

The use of two phase flow in lab-on-chip devices, where chemical and biological reagents are enclosed within plugs separated from each other by an immiscible fluid, offers significant advantages for the development of devices with high throughput of individual heterogeneous samples. Lab-on-chip devices designed to perform the polymerase chain reaction (PCR) are a prime example of such developments. The internal circulation within the plugs used to transport the reagents affects the efficiency of the chemical reaction within the plug, due to the degree of mixing induced on the reagents by the flow regime. It has been hypothesised in the literature that all plug flows produce internal circulation. This work demonstrates experimentally that this is false, and seeks to elucidate the parameters influencing the internal circulation of plugs. The particle image velocimetry (PIV) technique offers a powerful non-intrusive tool to study such flow fields. This paper presents micro-PIV experiments carried out to study the internal circulation of aqueous plugs in two phase flow within 762μm internal diameter FEP Teflon tubing with FC-40 as the segmenting fluid. Experiments have been performed and the results are presented for plugs ranging in length from 1mm to 13mm with an average fluid velocity ranging from 0.3mm/s to 50mm/s. The results demonstrate that circulation within the plugs is not always present and requires design considerations to benefit from this phenomenon.

scholarly journals The Fusion of Microfluidics and Optics for On-Chip Detection and Characterization of Microalgae

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1137
Author(s):  
Xinqi Zheng ◽  
Xiudong Duan ◽  
Xin Tu ◽  
Shulan Jiang ◽  
Chaolong Song
Keyword(s):  
Fossil Fuels ◽  
High Specificity ◽  
Microfluidic Chips ◽  
Optical Components ◽  
Lab On Chip ◽  
Metabolic Heterogeneity ◽  
On Chip ◽  
Micro Systems

It has been demonstrated that microalgae play an important role in the food, agriculture and medicine industries. Additionally, the identification and counting of the microalgae are also a critical step in evaluating water quality, and some lipid-rich microalgae species even have the potential to be an alternative to fossil fuels. However, current technologies for the detection and analysis of microalgae are costly, labor-intensive, time-consuming and throughput limited. In the past few years, microfluidic chips integrating optical components have emerged as powerful tools that can be used for the analysis of microalgae with high specificity, sensitivity and throughput. In this paper, we review recent optofluidic lab-on-chip systems and techniques used for microalgal detection and characterization. We introduce three optofluidic technologies that are based on fluorescence, Raman spectroscopy and imaging-based flow cytometry, each of which can achieve the determination of cell viability, lipid content, metabolic heterogeneity and counting. We analyze and summarize the merits and drawbacks of these micro-systems and conclude the direction of the future development of the optofluidic platforms applied in microalgal research.

Particle Focusing through Lab-on-Chip Device by Negative Dielectrophoresis

2010 ◽  
Vol 154-155 ◽  
pp. 287-291
Author(s):  
De Li Liu ◽  
Li Guo Chen ◽  
Chao Shao ◽  
Li Ning Sun
Keyword(s):  
Experimental Study ◽  
Computational Model ◽  
Electric Fields ◽  
Yeast Cells ◽  
Micro Channel ◽  
Lab On Chip ◽  
Focusing Effect ◽  
Particle Focusing ◽  
On Chip ◽  
Medium Conductivity

In this paper a Lab-On-Chip device using negative dielectrophoresis has been proposed for the focusing of particles at the center of the micro-channel. A 3D computational model was presented to simulate the focusing process. In particular, the model will solve for bioparticle movement and Joule heating. In the experimental study, the mixture of viable and nonviable yeast cells was used to demonstrate the focusing effect of the designed microfluidic device. The fabricated chip performs a desired focusing result for the conditions with the electric fields frequency of 1MHz, the medium conductivity of and the applied voltage , which validities the proposed 3D computational model. This Lab-On-Chip device provides a simple and effective mean of particle focusing.

Determination of Radiochemical Purity and Pharmacokinetic Parameters of 99mTc-Sulphur Colloid and 99mTc-Tin Colloid

1981 ◽  
Vol 20 (06) ◽  
pp. 279-282 ◽  
Author(s):  
D. Konstantinovska ◽  
K. Milivojević ◽  
J. Bzenić ◽  
V. Jovanović
Keyword(s):  
Radiochemical Purity ◽  
Slow Component ◽  
Pharmacokinetic Parameters ◽  
Optimum Size ◽  
Buffer Solution ◽  
Chromatography Method ◽  
Fast Phase ◽  
Biodistribution Studies ◽  
Biological Half Time

Labelling yield and radiochemical purity, higher than 95%, of 99mTc-colloid preparations were determined by using the paper chromatography method. Less than 3% of labelled citric acid, added to the preparation as a buffer solution, has been found in 99mTc-sulphur colloid. High radiochemical purity and optimum size of colloid particles has also been proved by biodistribution studies on experimental animals. The analysis performed has shown that more than 50% of 99mTc-colloid preparations excreted by urine is 99mTcO–, the remaining past 50% being protein bound 99mTc. Biological half-time of excretion of the fast phase is the same for both preparations, i.e. 10 min, while for the slow component it is 120 min in 99mTc-S-colloid and 160 min in 99mTc-Sn colloid.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

scholarly journals A Lab‐On‐chip Tool for Rapid, Quantitative, and Stage‐selective Diagnosis of Malaria

2021 ◽  
pp. 2004101
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  
Lab On Chip ◽  
On Chip
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