scholarly journals Determination of HMW – GS in wheat using SDS – PAGE and Lab-on-chip methods

10.5219/995 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 477-481 ◽  
Author(s):  
Timea Kuťka Hlozáková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Zdenka Gálová ◽  
Milan Chňapek ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Wheat Breeding ◽  
The Other ◽  
Gluten Protein ◽  
Gluten Proteins ◽  
Lab On Chip ◽  
Sds Page ◽  
Novel Complex ◽  
On Chip

SDS-PAGE is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoC (Lab-on-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. Benefits and limitations of Lab-on-Chip method over SDS-PAGE method in gluten proteins evaluation were explored in order to determine in which way LoC method should be improved in order to make its results more compliant with the results of SDS-PAGE. Chip electrophoresis provides a very good reproducibility of HMW-GS patterns. Moreover this approach is much faster than the conventional SDS-PAGE methods requiring several hours for an analysis. Another advantage over traditional gel electrophoresis is lower sample and reagent volume requirements, as well as specialized protein standards for accurate reproducibility and quantification. In the present study, we identified novel complex allele located at the locus Glu-1B.

scholarly journals New Exfoliative Toxin Produced by a Plasmid-Carrying Strain of Staphylococcus hyicus

1999 ◽  
Vol 67 (8) ◽  
pp. 4014-4018 ◽  
Author(s):  
Hisaaki Sato ◽  
Takao Watanabe ◽  
Yasuko Murata ◽  
Ayumi Ohtake ◽  
Mayumi Nakamura ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
The Other ◽  
Exfoliative Toxin ◽  
Sds Page ◽  
Serotype B

ABSTRACT A new serotype of Staphylococcus hyicus exfoliative toxin (SHET), serotype B, was isolated from the culture filtrate of a plasmid-carrying strain of S. hyicus. The new SHET was purified by precipitation with 70% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column, column chromatography on DEAE–Cellulofine A-500, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The new SHET caused exfoliation of the epidermis as determined by the so-called Nikolsky sign when inoculated into 1-day-old chickens. The new SHET was serologically different fromStaphylococcus aureus exfoliative toxins (ETs) (ETA, ETB, and ETC) and from the SHET from the plasmidless strain but showed the same molecular weight as the other serotypes of toxins on SDS-PAGE. It was thermolabile and lost its toxicity after being heated at 60°C for 30 min. We propose that the new SHET be designated SHETB and that the SHET produced by the plasmidless strain be designated SHETA.

Analysis of Electrokinetic Fluid Flow in T-Shaped DNA Chips

2012 ◽  
Author(s):  
Yogendra M. Panta ◽  
Sanket Aryal ◽  
Param C. Adhikari
Keyword(s):  
Electric Fields ◽  
Chemical Engineering ◽  
Fluid Velocity ◽  
Entire Solution ◽  
Ionic Concentration ◽  
Buffer Solution ◽  
Lab On Chip ◽  
On Chip ◽  
Novel Applications

Lab-on-chip devices promise for many novel applications concerning the transport of the liquid samples and other solutions in the order of micro-scale dimensions. One of the efficient methods for transporting fluid in the samples is through electrokinetic effects, where an electric field will be applied to charged ions such as DNA, a negatively charged ion or proteins. These ions are carried over in the microchannel by the application of electric fields through the entire solution from inlet via probe region for its detection to outlet and the determination of concentration distribution. COMSOL, commercially available multiphysics software, with its specific MEMS and Chemical Engineering modules were employed and simulated for the analysis of fluid velocity and ionic concentration throughout the channel of various shapes. The ionic fluid concentrations and velocities in the channel and at the outlet are plotted against the potential differences across the two inlets in which DNA sample was introduced from one inlet and a buffer solution was supplied from another inlet.

The αEC domain of human fibrinogen-420 is a stable and early plasmin cleavage product

Blood ◽  
2000 ◽  
Vol 95 (7) ◽  
pp. 2297-2303 ◽  
Author(s):  
Dianne Applegate ◽  
Lara Stoike Steben ◽  
Kathe M. Hertzberg ◽  
Gerd Grieninger
Keyword(s):  
Gel Electrophoresis ◽  
Degradation Products ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cleavage Product ◽  
The Other ◽  
Cross Linking ◽  
Human Fibrinogen ◽  
Western Blots ◽  
Sds Page

Abstract Human fibrinogen-420, (Eβγ)2, was isolated from plasma and evaluated for its ability to form clots and for its susceptibility to proteolysis. Clotting parameters, including cross-linking of subunit chains, of this subclass and of the more abundant fibrinogen-340 (βγ)2, were found to be similar, suggesting little impact of the unique EC domains of fibrinogen-420 on coagulation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of plasmic digestion patterns revealed production from fibrinogen-420 of the conventional fibrinogen degradation products, X, Y, D, and E, to be comparable to that from fibrinogen-340 in all respects except the presence of at least 2 additional cleavage products that were shown by Western blot analysis to contain the EC domain. One was a stable fragment (ECX) comigrating with a 34-kd yeast recombinant EC domain, and the other was an apparent precursor. Their release occurred early, before that of fragments D and E. Two bands of the same mobility and antibody reactivity were found in Western blots of plasma collected from patients with myocardial infarction shortly after the initiation of thrombolytic therapy.

scholarly journals Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Analysis of Palytoxin

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200
Author(s):  
Takahiro Abe ◽  
Takayuki Naito ◽  
Daisuke Uemura
Keyword(s):  
Public Health ◽  
Natural Products ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Food Poisoning ◽  
The Other ◽  
Sds Page ◽  
Drug Leads ◽  
Fish And Shellfish

Many natural products have been isolated from various marine organisms. These natural products, especially huge polyol and polyether compounds, are expected to be promising drug-leads. On the other hand, the accumulation of these compounds in fish and shellfish can cause food poisoning in humans. Therefore, the development of effective methods for the detection of these compounds is important from both academic and public health perspectives. We subjected palytoxin to an SDS-PAGE analysis, which is very easy, quick, and inexpensive, to determine whether this approach could be effective for detecting huge polyol natural products. Eventually, we were able to detect a band of palytoxin by SDS-PAGE analysis, which demonstrated that SDS-PAGE could be useful for detecting polyol and polyether compounds.

scholarly journals The Fusion of Microfluidics and Optics for On-Chip Detection and Characterization of Microalgae

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1137
Author(s):  
Xinqi Zheng ◽  
Xiudong Duan ◽  
Xin Tu ◽  
Shulan Jiang ◽  
Chaolong Song
Keyword(s):  
Fossil Fuels ◽  
High Specificity ◽  
Microfluidic Chips ◽  
Optical Components ◽  
Lab On Chip ◽  
Metabolic Heterogeneity ◽  
On Chip ◽  
Micro Systems

It has been demonstrated that microalgae play an important role in the food, agriculture and medicine industries. Additionally, the identification and counting of the microalgae are also a critical step in evaluating water quality, and some lipid-rich microalgae species even have the potential to be an alternative to fossil fuels. However, current technologies for the detection and analysis of microalgae are costly, labor-intensive, time-consuming and throughput limited. In the past few years, microfluidic chips integrating optical components have emerged as powerful tools that can be used for the analysis of microalgae with high specificity, sensitivity and throughput. In this paper, we review recent optofluidic lab-on-chip systems and techniques used for microalgal detection and characterization. We introduce three optofluidic technologies that are based on fluorescence, Raman spectroscopy and imaging-based flow cytometry, each of which can achieve the determination of cell viability, lipid content, metabolic heterogeneity and counting. We analyze and summarize the merits and drawbacks of these micro-systems and conclude the direction of the future development of the optofluidic platforms applied in microalgal research.

scholarly journals Light People: Tarik Bourouina

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Hui Wang ◽  
Xiaoyi Liu
Keyword(s):  
Climate Change ◽  
Sustainable Development Goals ◽  
Smart Cities ◽  
The Other ◽  
Research Experience ◽  
Global Pollution ◽  
Lab On Chip ◽  
Development Goals ◽  
On Chip ◽  
Set Up

EditorialIn 2015, 195 countries of the United Nations proposed Sustainable Development Goals so as to alleviate the problems of climate change and global pollution. In France, there is a scientist dedicated to contribute providing solutions for above issues by virtue of MEMS, Lab-On-Chip and metamaterials. This expert is Prof. Tarik Bourouina, a Professor of Physics at ESIEE Paris, Université Gustave Eiffel. He devoted himself to the investigations on micro sensors and metamaterials, and kept seeking their applications in the future blueprint of “Sustainable” and “Smart” cities. On the other hand, he has formed an indissoluble bond with Light: Science and Applications (LIGHT) since the very beginning of the journal. He also set up the LIGHT’s Paris office, which is the first LIGHT’s overseas office in Europe. We are much honored to have an opportunity to exclusively communicate with Prof. Tarik Bourouina, who will share his research experience and stories with LIGHT in this interview.

The αEC domain of human fibrinogen-420 is a stable and early plasmin cleavage product

Blood ◽  
2000 ◽  
Vol 95 (7) ◽  
pp. 2297-2303 ◽  
Author(s):  
Dianne Applegate ◽  
Lara Stoike Steben ◽  
Kathe M. Hertzberg ◽  
Gerd Grieninger
Keyword(s):  
Gel Electrophoresis ◽  
Degradation Products ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cleavage Product ◽  
The Other ◽  
Cross Linking ◽  
Human Fibrinogen ◽  
Western Blots ◽  
Sds Page

Human fibrinogen-420, (Eβγ)2, was isolated from plasma and evaluated for its ability to form clots and for its susceptibility to proteolysis. Clotting parameters, including cross-linking of subunit chains, of this subclass and of the more abundant fibrinogen-340 (βγ)2, were found to be similar, suggesting little impact of the unique EC domains of fibrinogen-420 on coagulation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of plasmic digestion patterns revealed production from fibrinogen-420 of the conventional fibrinogen degradation products, X, Y, D, and E, to be comparable to that from fibrinogen-340 in all respects except the presence of at least 2 additional cleavage products that were shown by Western blot analysis to contain the EC domain. One was a stable fragment (ECX) comigrating with a 34-kd yeast recombinant EC domain, and the other was an apparent precursor. Their release occurred early, before that of fragments D and E. Two bands of the same mobility and antibody reactivity were found in Western blots of plasma collected from patients with myocardial infarction shortly after the initiation of thrombolytic therapy.

Determination of the lattice translation across {110} APBs in GaAs

1988 ◽  
Vol 46 ◽  
pp. 600-601
Author(s):  
D.R. Rasmussen ◽  
N.-H. Cho ◽  
C.B. Carter
Keyword(s):  
Grain Boundaries ◽  
Lower Energy ◽  
Antiphase Boundary ◽  
The Other ◽  
Boundary Structure ◽  
Interface Plane ◽  
Inversion Symmetry ◽  
High Angle ◽  
Equilibrium Distance

Domains in GaAs can exist which are related to one another by the inversion symmetry, i.e., the sites of gallium and arsenic in one domain are interchanged in the other domain. The boundary between these two different domains is known as an antiphase boundary [1], In the terminology used to describe grain boundaries, the grains on either side of this boundary can be regarded as being Σ=1-related. For the {110} interface plane, in particular, there are equal numbers of GaGa and As-As anti-site bonds across the interface. The equilibrium distance between two atoms of the same kind crossing the boundary is expected to be different from the length of normal GaAs bonds in the bulk. Therefore, the relative position of each grain on either side of an APB may be translated such that the boundary can have a lower energy situation. This translation does not affect the perfect Σ=1 coincidence site relationship. Such a lattice translation is expected for all high-angle grain boundaries as a way of relaxation of the boundary structure.

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