Mechanical Characteristics of Tissue Engineered Bone-Ligament-Bone Constructs Following ACL Replacement in Sheep

2011 ◽  
Author(s):  
J. Ma ◽  
M. J. Smietana ◽  
E. M. Wojtys ◽  
L. M. Larkin ◽  
E. M. Arruda
Keyword(s):  
Mechanical Characteristics ◽  
Biomechanical Properties ◽  
Viable Option ◽  
Acl Repair ◽  
Acl Injuries ◽  
Tissue Constructs ◽  
Self Assembling ◽  
The Us ◽  
Engineered Tissue ◽  
Acl Replacement

With approximately 400,000 reported each year, anterior crucial ligament (ACL) injuries are the most common injury in the US. Unfortunately current ACL replacement strategies, which involve using either allografts from cadavers or autografts from patients’ own patellar tendons (PT) or hamstring tendons as a replacement, have several limitations including graft availability, risk of rejection, increased morbidity and, more importantly, unmatched intra-articular biomechanical properties of grafts and ACL. The objective of this study is to use self-assembling, scaffold-less bone-ligament-bone (BLB) engineered tissue constructs as grafts in a sheep ACL repair model to characterize the biomechanical behaviors of native ACL, PT, and tissue engineered ligament and subsequently present a viable option of using tissue engineered ligament graft for ACL repair.

Viscoelastic and Biomechanical Properties of Osteochondral Tissue Constructs Generated From Graded Polycaprolactone and Beta-Tricalcium Phosphate Composites

2010 ◽  
Vol 132 (9) ◽  
Author(s):  
Cevat Erisken ◽  
Dilhan M. Kalyon ◽  
Hongjun Wang
Keyword(s):  
Viscoelastic Material ◽  
Tricalcium Phosphate ◽  
Biomechanical Properties ◽  
Native Tissue ◽  
Tissue Constructs ◽  
Material Functions ◽  
Extracellular Matrix Components ◽  
Linear Viscoelastic ◽  
Engineered Tissue ◽  
Osteochondral Tissue

The complex micro-/nanostructure of native cartilage-to-bone insertion exhibits gradations in extracellular matrix components, leading to variations in the viscoelastic and biomechanical properties along its thickness to allow for smooth transition of loads under physiological movements. Engineering a realistic tissue for osteochondral interface would, therefore, depend on the ability to develop scaffolds with properly graded physical and chemical properties to facilitate the mimicry of the complex elegance of native tissue. In this study, polycaprolactone nanofiber scaffolds with spatially controlled concentrations of β-tricalcium phosphate nanoparticles were fabricated using twin-screw extrusion-electrospinning process and seeded with MC3T3-E1 cells to form osteochondral tissue constructs. The objective of the study was to evaluate the linear viscoelastic and compressive properties of the native bovine osteochondral tissue and the tissue constructs formed in terms of their small-amplitude oscillatory shear, unconfined compression, and stress relaxation behavior. The native tissue, engineered tissue constructs, and unseeded scaffolds exhibited linear viscoelastic behavior for strain amplitudes less than 0.1%. Both native tissue and engineered tissue constructs demonstrated qualitatively similar gel-like behavior as determined using linear viscoelastic material functions. The normal stresses in compression determined at 10% strain for the unseeded scaffold, the tissue constructs cultured for four weeks, and the native tissue were 0.87±0.08 kPa, 3.59±0.34 kPa, and 210.80±8.93 kPa, respectively. Viscoelastic and biomechanical properties of the engineered tissue constructs were observed to increase with culture time reflecting the development of a tissuelike structure. These experimental findings suggest that viscoelastic material functions of the tissue constructs can provide valuable inputs for the stages of in vitro tissue development.

scholarly journals The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5416-pdb.prot5416 ◽  
Author(s):  
R. A. Poche ◽  
J. E. Saik ◽  
J. L. West ◽  
M. E. Dickinson
Keyword(s):  
Live Imaging ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Transplantation Site

scholarly journals Noninvasive Quantification of In Vitro Osteoblastic Differentiation in 3D Engineered Tissue Constructs Using Spectral Ultrasound Imaging

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85749 ◽  
Author(s):  
Madhu Sudhan Reddy Gudur ◽  
Rameshwar R. Rao ◽  
Alexis W. Peterson ◽  
David J. Caldwell ◽  
Jan P. Stegemann ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Osteoblastic Differentiation ◽  
Tissue Constructs ◽  
Engineered Tissue

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

scholarly journals Vasculogenic dynamics in 3D engineered tissue constructs

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yaron J. Blinder ◽  
Alina Freiman ◽  
Noa Raindel ◽  
David J. Mooney ◽  
Shulamit Levenberg
Keyword(s):  
Tissue Constructs ◽  
Engineered Tissue

High-Resolution Cellular MRI: Gadolinium and Iron Oxide Nanoparticles for in-Depth Dual-Cell Imaging of Engineered Tissue Constructs

ACS Nano ◽  
2013 ◽  
Vol 7 (9) ◽  
pp. 7500-7512 ◽  
Author(s):  
Riccardo Di Corato ◽  
Florence Gazeau ◽  
Catherine Le Visage ◽  
Delphine Fayol ◽  
Pierre Levitz ◽  
...  
Keyword(s):  
Iron Oxide ◽  
High Resolution ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Cellular Mri ◽  
Tissue Constructs ◽  
Engineered Tissue

scholarly journals Tissue Engineering of Canine Cartilage from Surgically Debrided Osteochondritis Dissecans Fragments

2021 ◽  
Author(s):  
Natalia Vapniarsky ◽  
Lilia Moncada ◽  
Carissa Garrity ◽  
Alice Wong ◽  
Barbro Filliquist ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Thyroid Hormone ◽  
Cartilage Tissue Engineering ◽  
Collagen Content ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
Primary Chondrocytes ◽  
Native Tissue ◽  
Tissue Constructs ◽  
Self Assembling

AbstractThis study in dogs explored the feasibility of using cartilage fragments removed and discarded during routine palliative surgery for osteochondritis dissecans (OCD) as a source of primary chondrocytes for scaffold-free cartilage tissue-engineering. Primary chondrocytes were obtained from three OCD donors and one age-matched healthy articular cartilage (HAC) donor. After monolayer expansion of primary cells, a three-dimensional spherical suspension culture was implemented. Following this stage, cells were seeded at a high density into custom-made agarose molds that allowed for size and shape-specific constructs to be generated via a method of cellular self-assembling in a scaffold-free environment. Fifty-eight neocartilage constructs were tissue-engineered using this methodology. Neocartilage constructs and native cartilage from shoulder joint were subjected to histological, mechanical, and biochemical testing. OCD and HAC chondrocytes-sourced constructs had uniformly flat morphology and histology consistent with cartilage tissue. Constructs sourced from OCD chondrocytes were 1.5-times (32%) stiffer in compression and 1.3 times (23%) stronger in tension than constructs sourced from HAC chondrocytes and only 8.7-times (81%) less stiff in tension than native tissue. Constructs from both cell sources consistently had lower collagen content than native tissue (22.9%/dry weight [DW] for OCD and 4.1%/DW for HAC vs. 51.1%/DW native tissue). To improve the collagen content and mechanical properties of neocartilage, biological and mechanical stimuli, and thyroid hormone (tri-iodothyronine) were applied to the chondrocytes during the self-assembling stage in two separate studies. A 2.6-fold (62%) increase in compressive stiffness was detected with supplementation of biological stimuli alone and 5-fold (81%) increase with combined biological and mechanical stimuli at 20% strain. Application of thyroid hormone improved collagen content (1.7-times, 33%), tensile strength (1.8-times, 43%), and stiffness (1.3-times, 21%) of constructs, relative to untreated controls. Collectively, these data suggest that OCD chondrocytes can serve as a reliable cell source for cartilage tissue-engineering and that canine chondrocytes respond favorably to biological and mechanical stimuli that have been shown effective in chondrocytes from other animal species, including humans.

The Effect of Patellar Orientation on the Stiffness of the Porcine Femur-Medial Patellofemoral Ligament-Patella Complex

2011 ◽  
Author(s):  
Kwang E. Kim ◽  
Shanlin Hsu ◽  
Matthew B. Fisher ◽  
Savio L-Y. Woo
Keyword(s):  
Patellar Dislocation ◽  
Medial Patellofemoral Ligament ◽  
Primary Repair ◽  
Biomechanical Properties ◽  
The Us ◽  
Follow Up Studies ◽  
Graft Tissue ◽  
Redislocation Rate ◽  
Selection Of

Patellar dislocation has an incidence of 5.8 per 100,000 per year in the US, and this number increases by five-fold for adolescents 1. Conservative management has been the preferred treatment, but recent follow-up studies have revealed a high redislocation rate. Similar outcomes were also found following primary repair of the medial patellofemoral ligament (MPFL) 2. As a result, replacement with soft tissue autografts has gained attention for recurrent patellar dislocations in recent years 2, 3. As such, the selection of an appropriate graft tissue will need the knowledge of the biomechanical properties of the FMPC, particularly its stiffness for the performance of the graft.

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