scholarly journals The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5416-pdb.prot5416 ◽  
Author(s):  
R. A. Poche ◽  
J. E. Saik ◽  
J. L. West ◽  
M. E. Dickinson
Keyword(s):  
Live Imaging ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Transplantation Site

scholarly journals Noninvasive Quantification of In Vitro Osteoblastic Differentiation in 3D Engineered Tissue Constructs Using Spectral Ultrasound Imaging

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85749 ◽  
Author(s):  
Madhu Sudhan Reddy Gudur ◽  
Rameshwar R. Rao ◽  
Alexis W. Peterson ◽  
David J. Caldwell ◽  
Jan P. Stegemann ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Osteoblastic Differentiation ◽  
Tissue Constructs ◽  
Engineered Tissue

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

scholarly journals Vasculogenic dynamics in 3D engineered tissue constructs

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yaron J. Blinder ◽  
Alina Freiman ◽  
Noa Raindel ◽  
David J. Mooney ◽  
Shulamit Levenberg
Keyword(s):  
Tissue Constructs ◽  
Engineered Tissue

High-Resolution Cellular MRI: Gadolinium and Iron Oxide Nanoparticles for in-Depth Dual-Cell Imaging of Engineered Tissue Constructs

ACS Nano ◽  
2013 ◽  
Vol 7 (9) ◽  
pp. 7500-7512 ◽  
Author(s):  
Riccardo Di Corato ◽  
Florence Gazeau ◽  
Catherine Le Visage ◽  
Delphine Fayol ◽  
Pierre Levitz ◽  
...  
Keyword(s):  
Iron Oxide ◽  
High Resolution ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Cellular Mri ◽  
Tissue Constructs ◽  
Engineered Tissue

scholarly journals In vivo tissue engineering of a trilayered leaflet-shaped tissue construct

2020 ◽  
Vol 15 (1) ◽  
pp. 1177-1192
Author(s):  
Soumen Jana ◽  
Amir Lerman
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Tissue Constructs ◽  
In Vivo Tissue Engineering ◽  
Engineered Tissue ◽  
Tissue Construct

Aim: We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. Materials & methods: Electrospinning and in vivo tissue engineering methods were employed. Results: We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months in vivo tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. Conclusion: Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.

scholarly journals Matrix Production and Organization by Endothelial Colony Forming Cells in Mechanically Strained Engineered Tissue Constructs

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73161 ◽  
Author(s):  
Nicky de Jonge ◽  
Dimitri E. P. Muylaert ◽  
Emanuela S. Fioretta ◽  
Frank P. T. Baaijens ◽  
Joost O. Fledderus ◽  
...  
Keyword(s):  
Endothelial Colony Forming Cells ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Matrix Production
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