High-Resolution Cellular MRI: Gadolinium and Iron Oxide Nanoparticles for in-Depth Dual-Cell Imaging of Engineered Tissue Constructs

ACS Nano ◽  
2013 ◽  
Vol 7 (9) ◽  
pp. 7500-7512 ◽  
Author(s):  
Riccardo Di Corato ◽  
Florence Gazeau ◽  
Catherine Le Visage ◽  
Delphine Fayol ◽  
Pierre Levitz ◽  
...  
Keyword(s):  
Iron Oxide ◽  
High Resolution ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Cellular Mri ◽  
Tissue Constructs ◽  
Engineered Tissue

Extraction of Iron Oxide Nanoparticles from 3 Dimensional MRI Images UsingK-Mean Algorithm

2020 ◽  
Vol 15 (3) ◽  
pp. 369-375
Author(s):  
Reem S. Alanazi ◽  
Ali S. Saad
Keyword(s):  
Drug Delivery ◽  
Image Processing ◽  
Iron Oxide ◽  
High Resolution ◽  
Iron Oxide Nanoparticles ◽  
Clustering Algorithm ◽  
Region Of Interest ◽  
Diagnosis And Treatment ◽  
Therapeutic Drug ◽  
Oxide Nanoparticles

Nanomedicine targeted drug delivery is one of the emerging techniques for diagnosis and treatment of complex diseases. Medical image processing of High-Resolution Magnetic Resonance Imaging (HR-MRI), when combined with iron oxide nanoparticles (IO-NPs), provides a precious tool to monitor diagnosis and treatment processes. The challenge is to detect the nanoparticles inside the HR-MRI images. This is due to the low resolution of the images and the small size of the nanoparticles. In this paper, we study the drug delivery efficiency using a mouse with an inflamed calf, with IO-NPs attached to the therapeutic drug and injected into the mouse's eye. Our aim is to know how much of the drug injected will reach the inflamed region of the calf. A high-resolution MRI system was used to take images of the inflamed calf region. Knowing that iron oxide has a strong magnetic intensity on MRI images, image processing techniques were used to identify the location and quantity of IO-NPs attached to the drug. By knowing the location and quantity of IO-NPs we can estimate the quantity of drug delivered to the region of interest. In our project, K-mean algorithm, an automatic clustering algorithm was used to detect the iron oxide NPs in the MRI images. This then extracts them from the 3D model of the femoral region of interest. Extraction of NPs permits an estimation of the number of NPs clustered in the region and furthermore estimates the quantity of the drug delivered to the region of interest. The results obtained of nanoparticle detection and extraction seem to be a promising way to estimate the amount of delivered drug to a targeted area.

A simple assay for probing transformations of superparamagnetic iron oxide nanoparticles in human serum

2019 ◽  
Vol 55 (29) ◽  
pp. 4270-4272 ◽  
Author(s):  
Olga V. Kuznetsova ◽  
Irina S. Reshetnikova ◽  
Sergei N. Shtykov ◽  
Vasilii K. Karandashev ◽  
Bernhard K. Keppler ◽  
...  
Keyword(s):  
Iron Oxide ◽  
High Resolution ◽  
Human Serum ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Novel Approach ◽  
Icp Ms

A novel approach combining ultrafiltration and high-resolution ICP-MS was proposed for assessing the biocompatibility and biological identity of nanoparticles.

Efficient Synthesis of Water-Soluble Magnetic Nanoparticles and Its Application in MRI in the Detection of Intracranial Aneurysm

2021 ◽  
Vol 13 (9) ◽  
pp. 1699-1707
Author(s):  
Boya Li ◽  
Aihua Xiong ◽  
Xiaotong Yang ◽  
Qiong Yang ◽  
Jing Liu
Keyword(s):  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Intracranial Aneurysm ◽  
Iron Oxide Nanoparticles ◽  
Water Soluble ◽  
Oxide Nanoparticles ◽  
Aneurysm Wall ◽  
The Impact

Magnetic nanoparticles were used in medical images, which could further improve image clarity, while watersoluble nanoparticles put forward more new requirements for the biocompatibility of nanoparticles. This research adopted a simple and novel method to prepare water-soluble iron oxide nanoparticles. First, transmission electron microscope (TEM) was used to analyze the size distribution of the prepared product; X-ray diffraction (XRD) was used to test the crystal structure of the prepared sample; the fast Fourier transform (FFT) spectrum was introduced to analyze the structural properties of the nanoparticles; the nanoparticle aqueous solutions of different concentrations were designed, and the impact of water-soluble nanoparticles on magnetic resonance imaging (MRI) was examined with the nuclear magnetic resonance spectrometer. At the same time, the prepared water-soluble nanoparticle solution was used for high-resolution tumor wall imaging of patients with unruptured intracranial aneurysm (IA) to compare the imaging effect of the aneurysm wall before and after the introduction of nanoparticles. In the material characterization test of nanoparticles, the prepared samples did not have certain iron oxide characteristic peaks, which means the synthesized iron oxide nanoparticles did not have a fixed crystal morphology. The samples tested by energy dispersive spectrometer (EDS) also contained Fe, O, C and Na. The average particle size was 5.8 nm. It was found under high-resolution TEM that the particle mirror spacing was 0.48 nm, which was consistent with the 111-crystal plane of Fe3O4; The magnetic hysteresis loop test confirmed that when the concentration of nanoparticles increased, the solution would form a magnetic fluid. When the concentration of aqueous solution of nanoparticles increased, the corresponding MRI signal would be significantly enhanced. It was used in the MR scan of patients with unruptured IA. Nanoparticle solution could increase the visibility of the aneurysm, and the image quality of the aneurysm wall could be significantly enhanced.

Facile fabrication of glycopolymer-based iron oxide nanoparticles and their applications in the carbohydrate–lectin interaction and targeted cell imaging

2016 ◽  
Vol 7 (6) ◽  
pp. 1337-1344 ◽  
Author(s):  
Chen Shao ◽  
Xueming Li ◽  
Zhichao Pei ◽  
Dongdong Liu ◽  
Lin Wang ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Facile Fabrication ◽  
Novel Method

A novel method for facile fabrication of glycopolymer-based iron oxide nanoparticles (GIONs) is developed.

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

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