Design, Fabrication of High-Throughput Microarray Microfluidic Device for Membrane Protein Polyhedra Formation

2011 ◽  
Author(s):  
Hsin-Jui Wu ◽  
Yiwei Yan ◽  
Y. C. Lee ◽  
Michael Stowell
Keyword(s):  
Drug Delivery ◽  
Amino Acids ◽  
Membrane Protein ◽  
Microfluidic Device ◽  
High Throughput ◽  
Human Body ◽  
Medical Technology ◽  
Variable Number ◽  
Functional Element ◽  
Variable Geometry

In this paper, we designed and fabricated High-throughput Microfluidic device for membrane protein polyhedral. Protein is the most important functional element in our human body and also it could be applied to the key application areas of drug bonding and drug delivery. However protein stucture is difficult to be analyzed due to the complex and variable geometry of protein stucture which can be randomly formed by 20 amino acids and also plused 3D folding of stucture possibly. Based on this, we could imagine it would be a huge variable number of protein stucture, let’s say billion possibilities. Therefore if we can successful discover protein stucture, then we can expect that will improve drug delivery of medical technology forward to a big step.

Microfluidics of blood in blood vessels stenosis

2016 ◽  
Vol 11 (2) ◽  
pp. 210-217 ◽  
Author(s):  
A.T. Akhmetov ◽  
A.A. Valiev ◽  
A.A. Rakhimov ◽  
S.P. Sametov ◽  
R.R. Habibullina
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Hydraulic Resistance ◽  
Microfluidic Device ◽  
Human Body ◽  
Vascular System ◽  
Hydrodynamic Conditions ◽  
Microstructure Change ◽  
Healthy Part

It is mentioned in the paper that hydrodynamic conditions of a flow in blood vessels with the stenosis are abnormal in relation to the total hemodynamic conditions of blood flow in a vascular system of a human body. A microfluidic device developed with a stepped narrowing for studying of the blood flow at abnormal conditions allowed to reveal blood structure in microchannels simulating the stenosis. Microstructure change is observed during the flow of both native and diluted blood through the narrowing. The study of hemorheological properties allowed us to determine an increasing contribution of the hydraulic resistance of the healthy part of the vessel during the stenosis formation.

scholarly journals Micromechanical Punching: A Versatile Method for Non-Spherical Microparticle Fabrication

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 83
Author(s):  
Ritika Singh Petersen ◽  
Anja Boisen ◽  
Stephan Sylvest Keller
Keyword(s):  
Drug Delivery ◽  
High Resolution ◽  
Physicochemical Properties ◽  
High Throughput ◽  
Liquid State ◽  
Soft Lithography ◽  
Glycolic Acid ◽  
Thermosetting Polymers ◽  
Spherical Microparticle ◽  
Spherical Microparticles

Microparticles are ubiquitous in applications ranging from electronics and drug delivery to cosmetics and food. Conventionally, non-spherical microparticles in various materials with specific shapes, sizes, and physicochemical properties have been fabricated using cleanroom-free lithography techniques such as soft lithography and its high-resolution version particle replication in non-wetting template (PRINT). These methods process the particle material in its liquid/semi-liquid state by deformable molds, limiting the materials from which the particles and the molds can be fabricated. In this study, the microparticle material is exploited as a sheet placed on a deformable substrate, punched by a robust mold. Drawing inspiration from the macro-manufacturing technique of punching metallic sheets, Micromechanical Punching (MMP) is a high-throughput technique for fabrication of non-spherical microparticles. MMP allows production of microparticles from prepatterned, porous, and fibrous films, constituting thermoplastics and thermosetting polymers. As an illustration of application of MMP in drug delivery, flat, microdisk-shaped Furosemide embedded poly(lactic-co-glycolic acid) microparticles are fabricated and Furosemide release is observed. Thus, it is shown in the paper that Micromechanical punching has potential to make micro/nanofabrication more accessible to the research and industrial communities active in applications that require engineered particles.

High-Throughput Synthesis of Single-Layer MoS2 Nanosheets as a Near-Infrared Photothermal-Triggered Drug Delivery for Effective Cancer Therapy

ACS Nano ◽  
2014 ◽  
Vol 8 (7) ◽  
pp. 6922-6933 ◽  
Author(s):  
Wenyan Yin ◽  
Liang Yan ◽  
Jie Yu ◽  
Gan Tian ◽  
Liangjun Zhou ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
High Throughput ◽  
Near Infrared ◽  
Single Layer ◽  
Mos2 Nanosheets ◽  
Effective Cancer Therapy

scholarly journals A Region Within the Third Extracellular Loop of Rat AQP6 Precludes Its Trafficking to Plasma Membrane in a Heterologous Cell Line.

2021 ◽  
Author(s):  
David Soler ◽  
Thomas Kowatz ◽  
Andrew Sloan ◽  
Thomas McCormick ◽  
Kevin Cooper ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasma Membrane ◽  
Membrane Protein ◽  
Cell Line ◽  
Membrane Trafficking ◽  
Extracellular Loop ◽  
Reporter System ◽  
The Third ◽  
Heterologous Cell ◽  
Retention Signal

Abstract The inability to over-express AQP6 in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail. Using the AGR reporter system we have identified a region within loop C of AQP6 that is responsible for severely hampering its plasma membrane localization. Serine substitution corroborated that amino acids present within AQP6194-213 of AQP6 loop C contribute to intracellular retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous cells.

scholarly journals Assessment of L-Citrulline, L-Arginine and L-Glutamic Acid Content in Selected Fruits, Vegetables, Seeds, and Nuts Sold in Markets in Nairobi City County, Kenya

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Peninnah Mueni Mulwa ◽  
Wilson Njue ◽  
Margaret Ng’ang’a
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Human Body ◽  
Fruits And Vegetables ◽  
Brain Health ◽  
Button Mushroom ◽  
The Central Nervous System ◽  
Healing Of Wounds ◽  
Statistical Analysis Software ◽  
Internal Production

Background: L-citrulline, L-arginine, and L-glutamic acid are amino acids which are vital in the human body. L-citrulline boosts immunity, combats sarcopenia, detoxifies the liver, and enhances male fertility. L-arginine boosts internal production of nitric oxide, prevents abnormal blood clotting and accelerates healing of wounds. L-glutamic acid cleanses the central nervous system and improves overall brain health. Deficiency of these amino acids can lead to accumulation of ammonia and impact negatively to the nervous systems of the human body. This study aimed to identify and determine the levels of L-citrulline, L-arginine, and L-glutamic acid in selected fruits, vegetables, nuts, and seeds sold in markets in Nairobi City County, Kenya using Liquid Chromatography- Mass Spectrometry (LC-MS). Materials and Methods: Data was collected from 28 selected samples and data analysis was done using Statistical Analysis Software (SAS) version 9.4. Results: LC-MS showed the presence of both L-arginine and L-citrulline in most fruits, vegetables, and nuts while L-glutamic acid was present in the seeds. The levels of the amino acids in the fruits and vegetables were in the following range: L-citrulline (0.65- 19.41 mg/100g) in the button mushroom, cucumber, pumpkin, amaranthus, and kales, (3.16-3.79 mg/100g) in the watermelons and (1.57-10.21 mg/100g) in the nuts. L-arginine was in the range; 1.73 - 16.48 mg/100g in the amaranthus, kales, button mushroom, butternut squash, and cucumber, 5.44-6.56 mg/100g in the watermelons and 0.93-10.73 mg/100g in the nuts and L-glutamic acid (0.013-0.28 mg/100g) in the seeds of pumpkin, butternut, and watermelons. Conclusion: The results showed that locally available vegetables, fruits and nuts are rich in L-citrulline and L-arginine.

scholarly journals Design and Analysis of Mechanical Microswimmers

2021 ◽  
Keyword(s):  
Drug Delivery ◽  
Reynolds Number ◽  
Minimally Invasive ◽  
Human Body ◽  
Biomedical Applications ◽  
Head Capsule ◽  
Ansys Fluent ◽  
Capsule Type ◽  
Spherical Head ◽  
Surgical Operations

The fascinating field in present civilization scenario at the edge of science is micro-swimmers, which is a combination of bio physics with self-propulsion mechanisms involving swimming strategies at low Reynolds number. These micro swimming robots offer many advantages in biomedical applications such as drug delivery to some specific locations in our human body and also conducting some surgical operations like opening of blocked arteries etc. In recent times, blocked arteries become a major case in the medical world. This is can be diagnosed by Angioplasty (is a minimally invasive, endovascular procedure to widen narrowed or obstructed arteries or veins, typically to treat arterial atherosclerosis) method. So this is the main reason to choose the aorta as our domain for analysis purpose. This paper presents a micro-swimmer with three different heads they are spherical head, Capsule type head and Tapered cylindrical or elliptical head and modelled using SOLIDWORKS and analysis in ANSYS FLUENT.

scholarly journals Molecules and Functions of Cornus officinalis Bark Volatiles

2018 ◽  
pp. 828
Author(s):  
Wanxi Peng
Keyword(s):  
Amino Acids ◽  
Weight Loss ◽  
Human Body ◽  
Essential Elements ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Medicinal Material ◽  
Cornus Officinalis ◽  
Analysis Methods ◽  
Made In

Cornus officinalis Sieb. et Zucc is a traditional Chinese valuable medicinal material. Clinically, it is customary to use ripe fruits from which seeds have been removed for medicinal purposes. The pulp contains 16 amino acids and a large number of essential elements for the human body. In recent years, with the expansion of the application of cornus officinalis, its pharmacological and pharmacological effects have been increasingly studied. At present, significant achievements have been made in the study of the bioactive components of cornus officinalis. The research of these achievements has been based on the research of the fruit of cornus officinalis. The study of branches or bark of cornus officinalis is very rare. With the fruit of cornus officinalis getting more and more attention, in order to solve the problem of the shortage of cornus officinalis fruit in the market, in this paper, starting from the study of bark of cornus officinalis, TGA-DTG and PY-GC-MS analysis methods were used to study the weight loss and pyrolysis of cornus officinalis bark, providing a basis for more fully utilizing cornus officinalis resources. With reference.

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