Effects of Local Concentration on Freezing Solutions of Winter Flounder Antifreeze Protein

2011 ◽  
Author(s):  
Yoshimichi Hagiwara ◽  
Ryo Sakurai ◽  
Daichi Yamamoto ◽  
Atsuhide Kitagawa
Keyword(s):  
Interface Velocity ◽  
Antifreeze Protein ◽  
Winter Flounder ◽  
Local Concentration ◽  
Measured Intensity ◽  
Solution Interface ◽  
Inverted Microscope ◽  
Peltier Device ◽  
The One ◽  
Directional Freezing

We have carried out experiments on the one-directional freezing of an aqueous solution of winter flounder antifreeze protein in a narrow gap between two cover glasses. The motion of the ice/solution interface has been observed with an inverted microscope. The solution has been cooled by a Peltier device. The local change in protein concentration has been estimated from the measured intensity of fluorescence from molecules tagged to the protein. It is found that highly-concentrated regions of the protein can be observed in the bottom edge of the serrated interface. These regions interact with the interface, though most of the protein diffuses due to the concentration gradient. The diffusion velocity is much lower than the interface velocity. Thus, the protein is accumulated near the interface.

Grain boundary segregation in metals

1992 ◽  
Vol 50 (2) ◽  
pp. 1204-1205
Author(s):  
C.L. Briant
Keyword(s):  
Fracture Surface ◽  
Grain Boundary ◽  
Grain Boundaries ◽  
Material Properties ◽  
Electron Spectroscopy ◽  
High Vacuum ◽  
Boundary Segregation ◽  
Grain Boundary Segregation ◽  
Local Concentration ◽  
The One

Grain boundary segregation is the process by which solute elements in a material diffuse to the grain boundaries, become trapped there, and increase their local concentration at the boundary over that in the bulk. As a result of this process this local concentration of the segregant at the grain boundary can be many orders of magnitude greater than the bulk concentration of the segregant. The importance of this problem lies in the fact that grain boundary segregation can affect many material properties such as fracture, corrosion, and grain growth.One of the best ways to study grain boundary segregation is with Auger electron spectroscopy. This spectroscopy is an extremely surface sensitive technique. When it is used to study grain boundary segregation the sample must first be fractured intergranularly in the high vacuum spectrometer. This fracture surface is then the one that is analyzed. The development of scanning Auger spectrometers have allowed researchers to first image the fracture surface that is created and then to perform analyses on individual grain boundaries.

scholarly journals Ice Growth Suppression in the Solution Flows of Antifreeze Protein and Sodium Chloride in a Mini-Channel

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 306
Author(s):  
Kazuya Taira ◽  
Tomonori Waku ◽  
Yoshimichi Hagiwara
Keyword(s):  
Sodium Chloride ◽  
Synergistic Effects ◽  
Pure Water ◽  
Interface Velocity ◽  
Antifreeze Protein ◽  
Layer Growth ◽  
Mixed Solution ◽  
Solution Flow ◽  
Ice Growth ◽  
Cold Energy

The control of ice growth inside channels of aqueous solution flows is important in numerous fields, including (a) cold-energy transportation plants and (b) the preservation of supercooled human organs for transplantation. A promising method for this control is to add a substance that influences ice growth in the flows. However, limited results have been reported on the effects of such additives. Using a microscope, we measured the growth of ice from one sidewall toward the opposite sidewall of a mini-channel, where aqueous solutions of sodium chloride and antifreeze protein flowed. Our aim was to considerably suppress ice growth by mixing the two solutes. Inclined interfaces, the overlapping of serrated interfaces, and interfaces with sharp and flat tips were observed in the cases of the protein-solution, salt-solution, and mixed-solution flows, respectively. In addition, it was found that the average interface velocity in the case of the mixed-solution flow was the lowest and decreased by 64% compared with that of pure water. This significant suppression of the ice-layer growth can be attributed to the synergistic effects of the ions and antifreeze protein on the diffusion of protein.

Induction of Winter Flounder Antifreeze Protein Messenger RNA at 4 C in vivo and in vitro

1986 ◽  
Vol 59 (6) ◽  
pp. 679-695 ◽  
Author(s):  
Jeffrey L. Price ◽  
Brian B. Gourlie ◽  
Yuan Lin ◽  
Ru Chih C. Huang
Keyword(s):  
Messenger Rna ◽  
Antifreeze Protein ◽  
Winter Flounder

scholarly journals Ice-binding structure and mechanism of an antifreeze protein from winter flounder

Nature ◽  
1995 ◽  
Vol 375 (6530) ◽  
pp. 427-431 ◽  
Author(s):  
F. Sicheri ◽  
D. S. C. Yang
Keyword(s):  
Antifreeze Protein ◽  
Winter Flounder ◽  
Ice Binding ◽  
Binding Structure

Winter Flounder Antifreeze Protein Improves the Cold Hardiness of Plant Tissues

1989 ◽  
Vol 135 (3) ◽  
pp. 351-354 ◽  
Author(s):  
Adrian J. Cutler ◽  
M. Saleem ◽  
Edward Kendall ◽  
Lawrence V. Gusta ◽  
F. Georges ◽  
...  
Keyword(s):  
Cold Hardiness ◽  
Antifreeze Protein ◽  
Winter Flounder ◽  
Plant Tissues

scholarly journals Universal evaporation dynamics of ordered arrays of sessile droplets

2019 ◽  
Vol 866 ◽  
pp. 61-81 ◽  
Author(s):  
Sandeep Hatte ◽  
Keshav Pandey ◽  
Khushboo Pandey ◽  
Suman Chakraborty ◽  
Saptarshi Basu
Keyword(s):  
Near Field ◽  
Scaling Law ◽  
Present Theory ◽  
Solid Substrate ◽  
Local Concentration ◽  
Sessile Droplet ◽  
Droplet Array ◽  
Ordered Array ◽  
The One ◽  
Evaporation Dynamics

Manipulation of an array of surface droplets organised in an ordered structure turns out to be of immense consequence in a wide variety of applications ranging from photonics, near field imaging and inkjet printing on the one hand to bio-molecular analysis and DNA sequencing on the other. While evaporation of a single isolated sessile droplet has been well studied, the collective evaporative dynamics of an ordered array of droplets on a solid substrate remains elusive. Physically, the closed region between the centre and side droplets in the ordered array reduces the mobility of the diffusing vapour, resulting in its accumulation along with enhanced local concentration and a consequent increment in the lifetime of the centre droplet. Here, we present a theoretical model to account for evaporation lifetime scaling in closely placed ordered linear droplet arrays. In addition, the present theory predicts the limiting cases of droplet interaction; namely, critical droplet separation for which interfacial interaction ceases to exist and minimum possible droplet separation (droplets on the verge of coalescence) for which the droplet system achieves maximum lifetime scaling. Further experimental evidence demonstrates the applicability of the present scaling theory to extended dimensions of the droplet array, generalising our physical conjecture. It is also worth noting that the theoretical time scale is applicable across a wide variety of drop–substrate combinations and initial droplet volumes. We also highlight that the scaling law proposed here can be extended seamlessly to other forms of confinement such as an evaporating droplet inside a mini-channel, as encountered in countless applications ranging from biomedical engineering to surface patterning.

Sign in / Sign up

Export Citation Format

Share Document