A Validation Study of the Repeatability and Accuracy of Atomic Force Microscopy Indentation Using Polyacrylamide Gels and Colloidal Probes

2017 ◽  
Vol 139 (4) ◽  
Author(s):  
Donghee Lee ◽  
Sangjin Ryu
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Materials ◽  
Time Intervals ◽  
Indentation Method ◽  
Force Microscopy ◽  
Afm Indentation ◽  
Atomic Force ◽  
Comparable Accuracy ◽  
Soft Biological Materials ◽  
Colloidal Probes

The elasticity of soft biological materials is a critical property to understand their biomechanical behaviors. Atomic force microscopy (AFM) indentation method has been widely employed to measure the Young's modulus (E) of such materials. Although the accuracy of the method has been recently evaluated based on comparisons with macroscale E measurements, the repeatability of the method has yet to be validated for rigorous biomechanical studies of soft elastic materials. We tested the AFM indentation method using colloidal probes and polyacrylamide (PAAM) gels of E < 20 kPa as a model soft elastic material after having identified optimal trigger force and probe speed. AFM indentations repeated with time intervals show that the method is well repeatable when performed carefully. Compared with the rheometric method and the confocal microscopy indentation method, the AFM indentation method is evaluated to have comparable accuracy and better precision, although these elasticity measurements appear to rely on the compositions of PAAM gels and the length scale of measurement. Therefore, we have confirmed that the AFM indentation method can reliably measure the elasticity of soft elastic materials.

Quantitative mechanical analysis of indentations on layered, soft elastic materials

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1776-1784 ◽  
Author(s):  
Bryant L. Doss ◽  
Kiarash Rahmani Eliato ◽  
Keng-hui Lin ◽  
Robert Ros
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Cell Mechanics ◽  
Biological Samples ◽  
Mechanical Analysis ◽  
Elastic Materials ◽  
Mechanical Heterogeneity ◽  
Popular Method ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) is becoming an increasingly popular method for studying cell mechanics, however the existing analysis tools for determining the elastic modulus from indentation experiments are unable to quantitatively account for mechanical heterogeneity commonly found in biological samples.

Probing arsenic trioxide (ATO) treated leukemia cell elasticities using atomic force microscopy

2020 ◽  
Vol 12 (39) ◽  
pp. 4734-4741
Author(s):  
Hélène Fortier ◽  
Valerie Gies ◽  
Fabio Variola ◽  
Chen Wang ◽  
Shan Zou
Keyword(s):  
Atomic Force Microscopy ◽  
Drug Treatment ◽  
Arsenic Trioxide ◽  
Mechanical Response ◽  
Leukemia Cell ◽  
Indentation Method ◽  
Force Microscopy ◽  
Atomic Force

Nanomechanical indentation method to unveil the relationships among biochemical, structural, morphological, and mechanical response to arsenic trioxide drug treatment.

scholarly journals Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

2015 ◽  
Vol 86 (3) ◽  
pp. 033705 ◽  
Author(s):  
Luca Puricelli ◽  
Massimiliano Galluzzi ◽  
Carsten Schulte ◽  
Alessandro Podestà ◽  
Paolo Milani
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Colloidal Probes

Analysis of Indentation: Implications for Measuring Mechanical Properties With Atomic Force Microscopy

1999 ◽  
Vol 121 (5) ◽  
pp. 462-471 ◽  
Author(s):  
K. D. Costa ◽  
F. C. P. Yin
Keyword(s):  
Indentation Depth ◽  
Constitutive Law ◽  
Elastic Materials ◽  
Force Microscopy ◽  
Linear Elastic ◽  
Afm Indentation ◽  
Atomic Force ◽  
Afm Probe ◽  
Nonlinear Elastic ◽  
Indenter Geometry

Indentation using the atomic force microscope (AFM) has potential to measure detailed micromechanical properties of soft biological samples. However, interpretation of the results is complicated by the tapered shape of the AFM probe tip, and its small size relative to the depth of indentation. Finite element models (FEMs) were used to examine effects of indentation depth, tip geometry, and material nonlinearity and heterogeneity on the finite indentation response. Widely applied infinitesimal strain models agreed with FEM results for linear elastic materials, but yielded substantial errors in the estimated properties for nonlinear elastic materials. By accounting for the indenter geometry to compute an apparent elastic modulus as a function of indentation depth, nonlinearity and heterogeneity of material properties may be identified. Furthermore, combined finite indentation and biaxial stretch may reveal the specific functional form of the constitutive law—a requirement for quantitative estimates of material constants to be extracted from AFM indentation data.

Measuring Viscoelasticity of Soft Samples Using Atomic Force Microscopy

2009 ◽  
Vol 131 (9) ◽  
Author(s):  
S. Tripathy ◽  
E. J. Berger
Keyword(s):  
Atomic Force Microscopy ◽  
Soft Tissues ◽  
Relaxation Times ◽  
Indentation Test ◽  
Strain History ◽  
Force Microscopy ◽  
Afm Indentation ◽  
Atomic Force ◽  
Dependent Part ◽  
Soft Samples

Relaxation indentation experiments using atomic force microscopy (AFM) are used to obtain viscoelastic material properties of soft samples. The quasilinear viscoelastic (QLV) model formulated by Fung (1972, “Stress Strain History Relations of Soft Tissues in Simple Elongation,” in Biomechanics, Its Foundation and Objectives, Prentice-Hall, Englewood Cliffs, NJ, pp. 181–207) for uniaxial compression data was modified for the indentation test data in this study. Hertz contact mechanics was used for the instantaneous deformation, and a reduced relaxation function based on continuous spectrum is used for the time-dependent part in the model. The modified QLV indentation model presents a novel method to obtain viscoelastic properties from indentation data independent of relaxation times of the test. The major objective of the present study is to develop the QLV indentation model and implement the model on AFM indentation data for 1% agarose gel and a viscoelastic polymer using spherical indenter.

An Atomic Force Microscopy Indentation Study of Biomaterial Properties

2005 ◽  
Author(s):  
E. J. Berger ◽  
S. Tripathy ◽  
K. Vemaganti ◽  
Y. M. Kolambkar ◽  
H. X. You ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Soft Materials ◽  
Data Interpretation ◽  
Close Relative ◽  
Small Scale ◽  
Force Microscopy ◽  
Afm Indentation ◽  
Atomic Force ◽  
Biomechanical Investigation ◽  
Series Type

Atomic force microscopy (AFM) is a powerful and increasingly common modality of biomechanical investigation, including imaging, force spectroscopy, and microrheology. AFM indentation of biomaterials requires use of a contact model for data interpretation and material property extraction, and a large segment of the scientific community uses the Hertz model or a close relative for small-scale indentation of thin, soft materials in high strain applications. We present experimental results and analytical/numerical modeling which lead to two main conclusions: (i) Hertzian mechanics are useful in a surprisingly large parameter range, including scenarios in which the underlying assumptions are seemingly violated, and (ii) the Hertz solution serves as a useful base from which power-series type solutions can be derived for a variety of non-Hertzian effects.

Calibration of colloidal probes with atomic force microscopy for micromechanical assessment

2018 ◽  
Vol 85 ◽  
pp. 225-236 ◽  
Author(s):  
Lukas Kain ◽  
Orestis G. Andriotis ◽  
Peter Gruber ◽  
Martin Frank ◽  
Marica Markovic ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Colloidal Probes

Probing the Role of Nanoroughness in Contact Mechanics by Atomic Force Microscopy

2006 ◽  
Vol 51 ◽  
pp. 90-98 ◽  
Author(s):  
Renato Buzio ◽  
Ugo Valbusa
Keyword(s):  
Atomic Force Microscopy ◽  
Contact Mechanics ◽  
Crucial Role ◽  
Experimental Results ◽  
Present Contribution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Colloidal Probes ◽  
Solid Bodies

Morphological information can be related to significant properties of solid bodies, like their friction, adhesion and wear. The primary aim of the present contribution is to provide evidences of the crucial role played by roughness in contact mechanics, based on Atomic Force Microscopy investigations at the nanoscale. We report experimental results concerning poly(dimethylsiloxane) colloidal probes indenting smooth substrates and discuss the dependence of load-penetration curves and pull-off forces on system details. We suggest their use to perform novel contact mechanics experiments on nanostructured rough surfaces.

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