scholarly journals Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

2015 ◽  
Vol 86 (3) ◽  
pp. 033705 ◽  
Author(s):  
Luca Puricelli ◽  
Massimiliano Galluzzi ◽  
Carsten Schulte ◽  
Alessandro Podestà ◽  
Paolo Milani
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Colloidal Probes

scholarly journals Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcos Penedo ◽  
Tetsuya Shirokawa ◽  
Mohammad Shahidul Alam ◽  
Keisuke Miyazawa ◽  
Takehiko Ichikawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Cell Biology ◽  
Cell Damage ◽  
Efficiency Analysis ◽  
Living Cells ◽  
Biomolecular Recognition ◽  
Cell Penetration ◽  
Force Microscopy ◽  
Atomic Force

AbstractOver the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.

scholarly journals Anisotropic Mechanical Properties of Living Cells Revealed by Integrated Spinning Disk Confocal and Atomic Force Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spinning Disk ◽  
Anisotropic Mechanical Properties

scholarly journals 3P-311 Viscoelastic Properties of Many Living Cells Investigated by Atomic Force Microscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S175
Author(s):  
Shinichiro Hiratsuka ◽  
Yusuke Mizutani ◽  
Masahiro Tsuchiya ◽  
Koichi Kawahara ◽  
Hiroshi Tokumoto ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Annual Meeting ◽  
Viscoelastic Properties ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biophysical Society

scholarly journals Continuous detection of extracellular ATP on living cells by using atomic force microscopy

1999 ◽  
Vol 96 (21) ◽  
pp. 12180-12185 ◽  
Author(s):  
S. W. Schneider ◽  
M. E. Egan ◽  
B. P. Jena ◽  
W. B. Guggino ◽  
H. Oberleithner ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Extracellular Atp ◽  
Force Microscopy ◽  
Atomic Force

Imidazolium-Based Ionic Liquids Affect Morphology and Rigidity of Living Cells: An Atomic Force Microscopy Study

Langmuir ◽  
2018 ◽  
Vol 34 (41) ◽  
pp. 12452-12462 ◽  
Author(s):  
Massimiliano Galluzzi ◽  
Carsten Schulte ◽  
Paolo Milani ◽  
Alessandro Podestà
Keyword(s):  
Atomic Force Microscopy ◽  
Ionic Liquids ◽  
Microscopy Study ◽  
Living Cells ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force
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