Probing arsenic trioxide (ATO) treated leukemia cell elasticities using atomic force microscopy

2020 ◽  
Vol 12 (39) ◽  
pp. 4734-4741
Author(s):  
Hélène Fortier ◽  
Valerie Gies ◽  
Fabio Variola ◽  
Chen Wang ◽  
Shan Zou
Keyword(s):  
Atomic Force Microscopy ◽  
Drug Treatment ◽  
Arsenic Trioxide ◽  
Mechanical Response ◽  
Leukemia Cell ◽  
Indentation Method ◽  
Force Microscopy ◽  
Atomic Force

Nanomechanical indentation method to unveil the relationships among biochemical, structural, morphological, and mechanical response to arsenic trioxide drug treatment.

scholarly journals Mechanical response of adherent giant liposomes to indentation with a conical AFM-tip

Soft Matter ◽  
2015 ◽  
Vol 11 (22) ◽  
pp. 4487-4495 ◽  
Author(s):  
Edith Schäfer ◽  
Marian Vache ◽  
Torben-Tobias Kliesch ◽  
Andreas Janshoff
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Mechanical Response ◽  
Force Microscopy ◽  
Atomic Force

Mechanical properties of giant liposomes with actin cortices are determined with atomic force microscopy.

scholarly journals Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales

ACS Nano ◽  
2015 ◽  
Vol 9 (6) ◽  
pp. 5846-5856 ◽  
Author(s):  
Annafrancesca Rigato ◽  
Felix Rico ◽  
Frédéric Eghiaian ◽  
Mathieu Piel ◽  
Simon Scheuring
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Response ◽  
Force Microscopy ◽  
Atomic Force

Curing dependent spatial heterogeneity of mechanical response in epoxy resins revealed by atomic force microscopy

Polymer ◽  
2015 ◽  
Vol 68 ◽  
pp. 1-10 ◽  
Author(s):  
Amir Bahrami ◽  
Xavier Morelle ◽  
Lê Duy Hông Minh ◽  
Thomas Pardoen ◽  
Christian Bailly ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Spatial Heterogeneity ◽  
Epoxy Resins ◽  
Mechanical Response ◽  
Force Microscopy ◽  
Atomic Force

Sub-cellular Dynamic Mechanical Response Measurements of Live Human Pulmonary Artery Endothelial Cells with Atomic Force Microscopy

2012 ◽  
Vol 18 (S2) ◽  
pp. 1622-1623
Author(s):  
S. Avasthy ◽  
Y. Ishikawa ◽  
G. Shekhawat ◽  
V.P. Dravid ◽  
G. Mustata ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Mechanical Response ◽  
Force Microscopy ◽  
Dynamic Mechanical ◽  
Atomic Force ◽  
Human Pulmonary Artery

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

scholarly journals On the frequency dependence of viscoelastic material characterization with intermittent-contact dynamic atomic force microscopy: avoiding mischaracterization across large frequency ranges

2020 ◽  
Vol 11 ◽  
pp. 1409-1418
Author(s):  
Enrique A López-Guerra ◽  
Santiago D Solares
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Response ◽  
Viscoelastic Behavior ◽  
Partial Solution ◽  
Electromagnetic Properties ◽  
Multiple Characteristic ◽  
Force Microscopy ◽  
Atomic Force ◽  
Linear Viscoelastic ◽  
Intermittent Contact

Atomic force microscopy (AFM) is a widely use technique to acquire topographical, mechanical, or electromagnetic properties of surfaces, as well as to induce surface modifications at the micrometer and nanometer scale. Viscoelastic materials, examples of which include many polymers and biological materials, are an important class of systems, the mechanical response of which depends on the rate of application of the stresses imparted by the AFM tip. The mechanical response of these materials thus depends strongly on the frequency at which the characterization is performed, so much so that important aspects of behavior may be missed if one chooses an arbitrary characterization frequency regardless of the materials properties. In this paper we present a linear viscoelastic analysis of intermittent-contact, nearly resonant dynamic AFM characterization of such materials, considering the possibility of multiple characteristic times. We describe some of the intricacies observed in their mechanical response and alert the reader about situations where mischaracterization may occur as a result of probing the material at frequency ranges or with probes that preclude observation of its viscoelastic behavior. While we do not offer a solution to the formidable problem of inverting the frequency-dependent viscoelastic behavior of a material from dynamic AFM observables, we suggest that a partial solution is offered by recently developed quasi-static force–distance characterization techniques, which incorporate viscoelastic models with multiple characteristic times and can help inform dynamic AFM characterization.

scholarly journals Nucleotides-Induced Changes in the Mechanical Properties of Living Endothelial Cells and Astrocytes, Analyzed by Atomic Force Microscopy

2021 ◽  
Vol 22 (2) ◽  
pp. 624
Author(s):  
Juan Carlos Gil-Redondo ◽  
Jagoba Iturri ◽  
Felipe Ortega ◽  
Raquel Pérez-Sen ◽  
Andreas Weber ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Young’S Modulus ◽  
Mechanical Response ◽  
Adhesion Force ◽  
Young's Modulus ◽  
Nucleotide Receptors ◽  
Receptor Stimulation ◽  
Force Microscopy ◽  
Atomic Force

Endothelial cells and astrocytes preferentially express metabotropic P2Y nucleotide receptors, which are involved in the maintenance of vascular and neural function. Among these, P2Y1 and P2Y2 receptors appear as main actors, since their stimulation induces intracellular calcium mobilization and activates signaling cascades linked to cytoskeletal reorganization. In the present work, we have analyzed, by means of atomic force microscopy (AFM) in force spectroscopy mode, the mechanical response of human umbilical vein endothelial cells (HUVEC) and astrocytes upon 2MeSADP and UTP stimulation. This approach allows for simultaneous measurement of variations in factors such as Young’s modulus, maximum adhesion force and rupture event formation, which reflect the potential changes in both the stiffness and adhesiveness of the plasma membrane. The largest effect was observed in both endothelial cells and astrocytes after P2Y2 receptor stimulation with UTP. Such exposure to UTP doubled the Young’s modulus and reduced both the adhesion force and the number of rupture events. In astrocytes, 2MeSADP stimulation also had a remarkable effect on AFM parameters. Additional studies performed with the selective P2Y1 and P2Y13 receptor antagonists revealed that the 2MeSADP-induced mechanical changes were mediated by the P2Y13 receptor, although they were negatively modulated by P2Y1 receptor stimulation. Hence, our results demonstrate that AFM can be a very useful tool to evaluate functional native nucleotide receptors in living cells.

scholarly journals Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 468
Author(s):  
Maria Maares ◽  
Claudia Keil ◽  
Leif Löher ◽  
Andreas Weber ◽  
Amsatou Andorfer-Sarr ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Cell Lines ◽  
Mechanical Response ◽  
Cultured Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Impact ◽  
Ht 29

Monitoring biomechanics of cells or tissue biopsies employing atomic force microscopy (AFM) offers great potential to identify diagnostic biomarkers for diseases, such as colorectal cancer (CRC). Data on the mechanical properties of CRC cells, however, are still scarce. There is strong evidence that the individual zinc status is related to CRC risk. Thus, this study investigates the impact of differing zinc supply on the mechanical response of the in vitro CRC cell lines HT-29 and HT-29-MTX during their early proliferation (24–96 h) by measuring elastic modulus, relaxation behavior, and adhesion factors using AFM. The differing zinc supply severely altered the proliferation of these cells and markedly affected their mechanical properties. Accordingly, zinc deficiency led to softer cells, quantitatively described by 20–30% lower Young’s modulus, which was also reflected by relevant changes in adhesion and rupture event distribution compared to those measured for the respective zinc-adequate cultured cells. These results demonstrate that the nutritional zinc supply severely affects the nanomechanical response of CRC cell lines and highlights the relevance of monitoring the zinc content of cancerous cells or biopsies when studying their biomechanics with AFM in the future.

Sign in / Sign up

Export Citation Format

Share Document