scholarly journals Examining Differences in Local Collagen Fiber Crimp Frequency Throughout Mechanical Testing in a Developmental Mouse Supraspinatus Tendon Model

2012 ◽  
Vol 134 (4) ◽  
Author(s):  
Kristin S. Miller ◽  
Brianne K. Connizzo ◽  
Elizabeth Feeney ◽  
Jennica J. Tucker ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Testing ◽  
Collagen Fiber ◽  
Mechanical Test ◽  
Nonlinear Behavior ◽  
Supraspinatus Tendon ◽  
Behavior Changes ◽  
Developmental Age ◽  
Sst Model ◽  
Custom Software ◽  
Number Of Cycles

Crimp morphology is believed to be related to tendon mechanical behavior. While crimp has been extensively studied at slack or nondescript load conditions in tendon, few studies have examined crimp at specific, quantifiable loading conditions. Additionally, the effect of the number of cycles of preconditioning on collagen fiber crimp behavior has not been examined. Further, the dependence of collagen fiber crimp behavior on location and developmental age has not been examined in the supraspinatus tendon. Local collagen fiber crimp frequency is quantified throughout tensile mechanical testing using a flash freezing method immediately following the designated loading protocol. Samples are analyzed quantitatively using custom software and semi-quantitatively using a previously established method to validate the quantitative software. Local collagen fiber crimp frequency values are compared throughout the mechanical test to determine where collagen fiber frequency changed. Additionally, the effect of the number of preconditioning cycles is examined compared to the preload and toe-region frequencies to determine if increasing the number of preconditioning cycles affects crimp behavior. Changes in crimp frequency with age and location are also examined. Decreases in collagen fiber crimp frequency were found at the toe-region at all ages. Significant differences in collagen fiber crimp frequency were found between the preload and after preconditioning points at 28 days. No changes in collagen fiber crimp frequency were found between locations or between 10 and 28 days old. Local collagen fiber crimp frequency throughout mechanical testing in a postnatal developmental mouse SST model was measured. Results confirmed that the uncrimping of collagen fibers occurs primarily in the toe-region and may contribute to the tendon’s nonlinear behavior. Additionally, results identified changes in collagen fiber crimp frequency with an increasing number of preconditioning cycles at 28 days, which may have implications on the measurement of mechanical properties and identifying a proper reference configuration.

scholarly journals Effect of Preconditioning and Stress Relaxation on Local Collagen Fiber Re-Alignment: Inhomogeneous Properties of Rat Supraspinatus Tendon

2012 ◽  
Vol 134 (3) ◽  
Author(s):  
Kristin S. Miller ◽  
Lena Edelstein ◽  
Brianne K. Connizzo ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Properties ◽  
Stress Relaxation ◽  
Mechanical Testing ◽  
Collagen Fiber ◽  
Mechanical Test ◽  
Optical Transition ◽  
Insertion Site ◽  
Polarized Light ◽  
Supraspinatus Tendon ◽  
Circular Variance

Repeatedly and consistently measuring the mechanical properties of tendon is important but presents a challenge. Preconditioning can provide tendons with a consistent loading history to make comparisons between groups from mechanical testing experiments. However, the specific mechanisms occurring during preconditioning are unknown. Previous studies have suggested that microstructural changes, such as collagen fiber re-alignment, may be a result of preconditioning. Local collagen fiber re-alignment is quantified throughout tensile mechanical testing using a testing system integrated with a polarized light setup, consisting of a backlight, 90 deg-offset rotating polarizer sheets on each side of the test sample, and a digital camera, in a rat supraspinatus tendon model, and corresponding mechanical properties are measured. Local circular variance values are compared throughout the mechanical test to determine if and where collagen fiber re-alignment occurred. The inhomogeneity of the tendon is examined by comparing local circular variance values, optical moduli and optical transition strain values. Although the largest amount of collagen fiber re-alignment was found during preconditioning, significant re-alignment was also demonstrated in the toe and linear regions of the mechanical test. No significant changes in re-alignment were seen during stress relaxation. The insertion site of the supraspinatus tendon demonstrated a lower linear modulus and a more disorganized collagen fiber distribution throughout all mechanical testing points compared to the tendon midsubstance. This study identified a correlation between collagen fiber re-alignment and preconditioning and suggests that collagen fiber re-alignment may be a potential mechanism of preconditioning and merits further investigation. In particular, the conditions necessary for collagen fibers to re-orient away from the direction of loading and the dependency of collagen reorganization on its initial distribution must be examined.

Collagen Fiber Re-Alignment and Mechanical Properties in a Mouse Supraspinatus Tendon Model: Examining Changes With Age and Location

2012 ◽  
Author(s):  
Kristin S. Miller ◽  
Brianne K. Connizzo ◽  
Elizabeth Feeney ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Properties ◽  
Collagen Fiber ◽  
Mechanical Load ◽  
Mechanical Test ◽  
Insertion Site ◽  
Supraspinatus Tendon ◽  
Tissue Response ◽  
Mechanical Stimuli ◽  
Fiber Alignment ◽  
Collagen Fiber Alignment

One postulated mechanism of tendon structural response to mechanical load is collagen fiber re-alignment. Recently, where collagen fiber re-alignment occurs during a tensile mechanical test has been shown to vary by tendon age and location in a postnatal developmental mouse supraspinatus tendon (SST) model [1]. It is thought that as the tendon matures and its collagen fibril network, collagen cross-links and collagen-matrix interactions develop, its ability to respond quickly to mechanical stimuli hastens [1]. Additionally, the insertion site and midsubstance of postnatal SST may develop differently and at different rates, providing a potential explanation for differences in fiber re-alignment behaviors at the insertion site and midsubstance at postnatal developmental time points [1]. However, collagen fiber re-alignment behavior, in response to mechanical load at a mature age and in comparison to developmental ages, have not been examined. Therefore, the objectives of this study are to locally measure: 1) fiber re-alignment during preconditioning and tensile mechanical testing and 2) to compare local differences in collagen fiber alignment and corresponding mechanical properties to address tissue response to mechanical load in the mature and postnatal developmental mouse SST. We hypothesize that 1) 90 day tendons will demonstrate the largest shift in fiber re-alignment during preconditioning, but will also re-align during the toe- and linear-regions. Additionally, we hypothesize that 2) mechanical properties and initial collagen fiber alignment will be greater in the midsubstance of the tendon compared to the tendon-to-bone insertion site at 90 days, 3) that mechanical properties will increase with age, and that 4) collagen fiber organization at the insertion site will decrease with age.

scholarly journals Characterizing local collagen fiber re-alignment and crimp behavior throughout mechanical testing in a mature mouse supraspinatus tendon model

2012 ◽  
Vol 45 (12) ◽  
pp. 2061-2065 ◽  
Author(s):  
Kristin S. Miller ◽  
Brianne K. Connizzo ◽  
Elizabeth Feeney ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Testing ◽  
Collagen Fiber ◽  
Supraspinatus Tendon ◽  
Mature Mouse

scholarly journals Effect of Age and Proteoglycan Deficiency on Collagen Fiber Re-Alignment and Mechanical Properties in Mouse Supraspinatus Tendon

2013 ◽  
Vol 135 (2) ◽  
Author(s):  
Brianne K. Connizzo ◽  
Joseph J. Sarver ◽  
Renato V. Iozzo ◽  
David E. Birk ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Properties ◽  
Collagen Fiber ◽  
Mechanical Test ◽  
Insertion Site ◽  
Supraspinatus Tendon ◽  
Matrix Proteins ◽  
Wild Type ◽  
Structural Alterations ◽  
A Site ◽  
Effect Of Age

Collagen fiber realignment is one mechanism by which tendon responds to load. Re-alignment is altered when the structure of tendon is altered, such as in the natural process of aging or with alterations of matrix proteins, such as proteoglycan expression. While changes in re-alignment and mechanical properties have been investigated recently during development, they have not been studied in (1) aged tendons, or (2) in the absence of key proteoglycans. Collagen fiber re-alignment and the corresponding mechanical properties are quantified throughout tensile mechanical testing in both the insertion site and the midsubstance of mouse supraspinatus tendons in wild type (WT), decorin-null (Dcn-/-), and biglycan-null (Bgn-/-) mice at three different ages (90 days, 300 days, and 570 days). Percent relaxation was significantly decreased with age in the WT and Dcn-/- tendons, but not in the Bgn-/- tendons. Changes with age were found in the linear modulus at the insertion site where the 300 day group was greater than the 90 day and 570 day group in the Bgn-/- tendons and the 90 day group was smaller than the 300 day and 570 day groups in the Dcn-/- tendons. However, no changes in modulus were found across age in WT tendons were found. The midsubstance fibers of the WT and Bgn-/- tendons were initially less aligned with increasing age. The re-alignment was significantly altered with age in the WT tendons, with older groups responding to load later in the mechanical test. This was also seen in the Dcn-/- midsubstance and the Bgn-/- insertion, but not in the other locations. Although some studies have found changes in the WT mechanical properties with age, this study did not support those findings. However, it did show fiber re-alignment changes at both locations with age, suggesting a breakdown of tendon's ability to respond to load in later ages. In the proteoglycan-null tendons however, there were changes in the mechanical properties, accompanied only by location-dependent re-alignment changes, suggesting a site-specific role for these molecules in loading. Finally, changes in the mechanical properties did not occur in concert with changes in re-alignment, suggesting that typical mechanical property measurements alone are insufficient to describe how structural alterations affect tendon's response to load.

Full-Scale Reeling Tests of HFI Welded Line Pipe for Offshore Reel-Laying Installation

2014 ◽  
Author(s):  
Karl Christoph Meiwes ◽  
Susanne Höhler ◽  
Marion Erdelen-Peppler ◽  
Holger Brauer
Keyword(s):  
Mechanical Testing ◽  
Mechanical Test ◽  
Strength Properties ◽  
Full Scale ◽  
Bending Test ◽  
Small Scale ◽  
Pipe Material ◽  
Deformation Capacity ◽  
Line Pipe ◽  
Tension And Compression

During reel-laying repeated plastic strains are introduced into a pipeline which may affect strength properties and deformation capacity of the line pipe material. Conventionally the effect on the material is simulated by small-scale reeling simulation tests. For these, coupons are extracted from pipes that are loaded in tension and compression and thermally aged, if required. Afterwards, specimens for mechanical testing are machined from these coupons and tested according to the corresponding standards. Today customers often demand additional full-scale reeling simulation tests to assure that the structural pipe behavior meets the strain demands as well. Realistic deformations have to be introduced into a full-size pipe, followed by aging, sampling and mechanical testing comparable to small-scale reeling. In this report the fitness for use of a four-point-bending test rig for full-scale reeling simulation tests is demonstrated. Two high-frequency-induction (HFI) welded pipes of grade X65M (OD = 323.9 mm, WT = 15.9 mm) from Salzgitter Mannesmann Line Pipe GmbH (MLP) are bent with alternate loading. To investigate the influences of thermal aging from polymer-coating process one test pipe had been heat treated beforehand, in the same manner as if being PE-coated. After the tests mechanical test samples were machined out of the plastically strained pipes. A comparison of results from mechanical testing of material exposed to small- and full-scale reeling simulation is given. The results allow an evaluation of the pipe behavior as regards reeling ability and plastic deformation capacity.

Effect of Preconditioning on Collagen Fiber Recruitment: Inhomogeneous Properties of Rat Supraspinatus Tendon

2010 ◽  
Author(s):  
Kristin S. Miller ◽  
Lena Edelstein ◽  
Louis J. Soslowsky
Keyword(s):  
Mechanical Properties ◽  
Collagen Fiber ◽  
Insertion Site ◽  
Supraspinatus Tendon ◽  
Fiber Alignment ◽  
Rigorous Evaluation ◽  
Structural Alterations ◽  
Testing Protocol ◽  
Inhomogeneous Properties ◽  
Collagen Fiber Alignment

Cyclic preconditioning is a commonly accepted initial component of any tendon testing protocol. Preconditioning provides tendons with a consistent “history” and stress-strain results become repeatable allowing for rigorous evaluation and comparison. While it is widely accepted that preconditioning is important, changes that occur during preconditioning are not well understood. Micro-structural alterations, such as re-arrangement of collagen fibers, is one proposed mechanism of preconditioning [1,4]. However, this mechanism has not been examined. Therefore, the objective of this study is to locally measure: 1) fiber re-alignment during preconditioning, stress relaxation and tensile testing and 2) corresponding mechanical properties, to address mechanisms of preconditioning as well as tissue nonlinearity and inhomogeneity in the rat supraspinatus tendon. We hypothesize that 1) fiber re-alignment will be greatest in the toe region, but will also occur during preconditioning and 2) mechanical properties and initial collagen fiber alignment will be greater in the midsubstance location of the tendon compared to the tendon-to-bone insertion site.

Some Experimental Considerations of Stage Control in Digital Valves

2002 ◽  
Author(s):  
J. Ruan ◽  
R. Burton ◽  
P. Ukrainetz
Keyword(s):  
Nonlinear Behavior ◽  
Stepper Motor ◽  
Nonlinear Characteristics ◽  
Practical Applications ◽  
Variable Reluctance ◽  
Stepper Motors ◽  
Number Of Cycles ◽  
Cyclic Changes ◽  
Starting Characteristics ◽  
Spool Valve

Because of saturation and hysteresis of magnetic materials, nonlinear characteristics are commonly experienced in servo or proportional valves. These nonlinearities can substantially affect the performance of the valve in practical applications. In the presence of magnetic nonlinearities, the output signal (displacement or force) is dependent on the input current and the sign of its derivative. If the driving current to the electrical-to-mechanical interface device changes for a number of cycles, as in a stepper motor for example, then a series of reset points will occur as the current undergoes cyclic changes. At each reset point the original starting characteristics of the system are re-established. A large number of reset points across the full stroke of the spool results in a significant reduction in the nonlinear behavior; indeed, the characteristics of the valve approach those of a linear system. The approach in creating these multiple reset points has been defined by the authors as “stage control”. In this paper, stage control using variable reluctance and hybrid stepper motors is first discussed. For the variable reluctance stepper motor, the reset point occurs once at each step of the stepper motor, whereas it occurs twice in a single cycle in the hybrid types. Experiments using a spool valve as a load were designed to obtain the characteristics using stage control. It is demonstrated that with the introduction of stage control, nonlinearities, such as saturation and hysteresis, are greatly reduced, system stiffness is increased, and the positioning accuracy and resolution of the spool are improved. The effect of dither due to a “digital fragment” signal is also examined and found to be crucial in reducing the hysteresis and in improving the resolution accuracy.

scholarly journals Biaxial Tensile Testing and Constitutive Modeling of Human Supraspinatus Tendon

2012 ◽  
Vol 134 (2) ◽  
Author(s):  
Spencer E. Szczesny ◽  
John M. Peloquin ◽  
Daniel H. Cortes ◽  
Jennifer A. Kadlowec ◽  
Louis J. Soslowsky ◽  
...  
Keyword(s):  
Constitutive Model ◽  
Collagen Fiber ◽  
Supraspinatus Tendon ◽  
Angle Distribution ◽  
Fiber Angle ◽  
Biaxial Tensile ◽  
Uniaxial Testing ◽  
Biaxial Tensile Testing ◽  
Transverse Stresses ◽  
Fiber Organization

The heterogeneous composition and mechanical properties of the supraspinatus tendon offer an opportunity for studying the structure-function relationships of fibrous musculoskeletal connective tissues. Previous uniaxial testing has demonstrated a correlation between the collagen fiber angle distribution and tendon mechanics in response to tensile loading both parallel and transverse to the tendon longitudinal axis. However, the planar mechanics of the supraspinatus tendon may be more appropriately characterized through biaxial tensile testing, which avoids the limitation of nonphysiologic traction-free boundary conditions present during uniaxial testing. Combined with a structural constitutive model, biaxial testing can help identify the specific structural mechanisms underlying the tendon’s two-dimensional mechanical behavior. Therefore, the objective of this study was to evaluate the contribution of collagen fiber organization to the planar tensile mechanics of the human supraspinatus tendon by fitting biaxial tensile data with a structural constitutive model that incorporates a sample-specific angular distribution of nonlinear fibers. Regional samples were tested under several biaxial boundary conditions while simultaneously measuring the collagen fiber orientations via polarized light imaging. The histograms of fiber angles were fit with a von Mises probability distribution and input into a hyperelastic constitutive model incorporating the contributions of the uncrimped fibers. Samples with a wide fiber angle distribution produced greater transverse stresses than more highly aligned samples. The structural model fit the longitudinal stresses well (median R2 ≥ 0.96) and was validated by successfully predicting the stress response to a mechanical protocol not used for parameter estimation. The transverse stresses were fit less well with greater errors observed for less aligned samples. Sensitivity analyses and relatively affine fiber kinematics suggest that these errors are not due to inaccuracies in measuring the collagen fiber organization. More likely, additional strain energy terms representing fiber-fiber interactions are necessary to provide a closer approximation of the transverse stresses. Nevertheless, this approach demonstrated that the longitudinal tensile mechanics of the supraspinatus tendon are primarily dependent on the moduli, crimp, and angular distribution of its collagen fibers. These results add to the existing knowledge of structure-function relationships in fibrous musculoskeletal tissue, which is valuable for understanding the etiology of degenerative disease, developing effective tissue engineering design strategies, and predicting outcomes of tissue repair.

The Effect of Substrate on the Microstructure and Mechanical Behavior of Eutectic Indium-Tin

1993 ◽  
Vol 323 ◽  
Author(s):  
J. L. Freer Goldstein ◽  
J. W. Morris
Keyword(s):  
Mechanical Behavior ◽  
Mechanical Testing ◽  
Creep Deformation ◽  
Activation Energies ◽  
Behavior Changes ◽  
Stress Strain ◽  
Cu Substrates ◽  
Different Shapes

AbstractThis study was conducted in order to determine and understand the effect of substrate on eutectic In-Sn. Samples for mechanical testing were produced with either bare Cu or Ni on Cu substrates. Both the microstructure and the mechanical behavior are strongly dependent on substrate, with In-Sn on Cu having a non-uniform and irregular microstructure and In-Sn on Ni having a uniform, normal colony-based eutectic. Deformation is more uniform in the In-Sn on Ni, while it is concentrated along the length of the joint in the In-Sn on Cu. This is reflected in the different shapes of stress-strain curves between In-Sn on Cu and In-Sn on Ni. The stress exponents and activation energies for creep also vary with substrate. Creep deformation is governed by the In-rich γ phase for In-Sn on Cu and by the Sn-rich y phase for In-Sn on Ni. If In-Sn on Ni samples are aged, the microstructure coarsens and the mechanical behavior changes to resemble that of the as-cast In-Sn on Cu.

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