Precision Assessment of Biofluid Viscosity Measurements Using Molecular Rotors

2005 ◽  
Vol 127 (3) ◽  
pp. 450-454 ◽  
Author(s):  
Walter J. Akers ◽  
Mark A. Haidekker
Keyword(s):  
Fluorescence Emission ◽  
High Viscosity ◽  
Plasma Viscosity ◽  
Molecular Rotors ◽  
Solution Viscosity ◽  
Human Blood Plasma ◽  
Fluid Viscosity ◽  
Molecular Rotor ◽  
Test Set ◽  
Precision Assessment

Blood viscosity changes with many pathologic conditions, but its importance has not been fully investigated because the current methods of measurement are poorly suited for clinical applications. The use of viscosity-sensitive fluorescent molecular rotors to determine fluid viscosity in a nonmechanical manner has been investigated recently, but it is unknown how the precision of the fluorescence-based method compares to established mechanical viscometry. Human blood plasma viscosity was modulated with high-viscosity plasma expanders, dextran, pentastarch, and hetastarch. The samples were divided into a calibration and a test set. The relationship between fluorescence emission and viscosity was established using the calibration set. Viscosity of the test set was determined by fluorescence and by cone-and-plate viscometer, and the precision of both methods compared. Molecular rotor fluorescence intensity showed a power law relationship with solution viscosity. Mechanical measurements deviated from the theoretical viscosity value by less than 7.6%, while fluorescence-based measurements deviated by less than 6%. The average coefficient of variation was 6.9% (mechanical measurement) and 3.4% to 3.8% (fluorescence-based measurement, depending on the molecular rotor used). Fluorescence-based viscometry exhibits comparable precision to mechanical viscometry. Fluorescence viscometry does not apply shear and is therefore more practical for biofluids which have apparent non-Newtonian properties. In addition, fluorescence instrumentation makes very fast serial measurements possible, thus promising new areas of application in laboratory and clinical settings.

A Molecular Rotor as Viscosity Sensor in Aqueous Colloid Solutions

2004 ◽  
Vol 126 (3) ◽  
pp. 340-345 ◽  
Author(s):  
W. Akers ◽  
M. A. Haidekker
Keyword(s):  
Quantum Yield ◽  
Power Law ◽  
Emission Intensity ◽  
Molecular Rotors ◽  
Solution Viscosity ◽  
Colloid Solution ◽  
Fluid Viscosity ◽  
Molecular Rotor ◽  
Average Precision

Background: Molecular rotors exhibit viscosity-dependent quantum yield, allowing non-mechanical determination of fluid viscosity. We analyzed fluorescence in the presence of viscosity-modulating macromolecules several orders of magnitude larger than the rotor molecule. Method of approach: Fluorescence of aqueous starch solutions with a molecular rotor in solution was related to viscosity obtained in a cone-and-plate viscometer. Results: In dextran solutions, emission intensity was found to follow a power-law relationship with viscosity. Fluorescence in hydroxyethylstarch solutions showed biexponential behavior with different exponents at viscosities above and below 1.5 mPa s. Quantum yield was generally higher in hydroxyethylstarch than in dextran solutions. The power-law relationship was used to backcalculate viscosity from intensity with an average precision of 2.2% (range of −5.5% to 5.1%). Conclusions: This study indicates that hydrophilic molecular rotors are suitable as colloid solution viscosity probes after colloid-dependent calibration.

Plasma viscosity regulates systemic and microvascular perfusion during acute extreme anemic conditions

2006 ◽  
Vol 291 (5) ◽  
pp. H2445-H2452 ◽  
Author(s):  
Pedro Cabrales ◽  
Amy G. Tsai
Keyword(s):  
Flow Distribution ◽  
Capillary Density ◽  
High Viscosity ◽  
Plasma Viscosity ◽  
Functional Capillary Density ◽  
Microvascular Perfusion ◽  
Arterial Blood ◽  
Chamber Model ◽  
Kidney Liver ◽  
Window Chamber

The hamster window chamber model was used to study systemic and microvascular hemodynamic responses to extreme hemodilution with low- and high-viscosity plasma expanders (LVPE and HVPE, respectively) to determine whether plasma viscosity is a factor in homeostasis during extreme anemic conditions. Moderated hemodilution was induced by two isovolemic steps performed with 6% 70-kDa dextran until systemic hematocrit (Hct) was reduced to 18% ( level 2). In a third isovolemic step, hemodilution with LVPE (6% 70-kDa dextran, 2.8 cP) or HVPE (6% 500-kDa dextran, 5.9 cP) reduced Hct to 11%. Systemic parameters, cardiac output (CO), organ flow distribution, microhemodynamics, and functional capillary density, were measured after each exchange dilution. Fluorescent-labeled microspheres were used to measure organ (brain, heart, kidney, liver, lung, and spleen) and window chamber blood flow. Final blood and plasma viscosities after the entire protocol were 2.1 and 1.4 cP, respectively, for LVPE and 2.8 and 2.2 cP, respectively, for HVPE (baseline = 4.2 and 1.2 cP, respectively). HVPE significantly elevated mean arterial pressure and CO compared with LVPE but did not increase vascular resistance. Functional capillary density was significantly higher for HVPE [87% (SD 7) of baseline] than for LVPE [42% (SD 11) of baseline]. Increases in mean arterial blood pressure, CO, and shear stress-mediated factors could be responsible for maintaining organ and microvascular perfusion after exchange with HVPE compared with LVPE. Microhemodynamic data corresponded to microsphere-measured perfusion data in vital organs.

Gastric emptying of nondigestible solids in dogs: a hydrodynamic correlation

1990 ◽  
Vol 258 (1) ◽  
pp. G65-G72 ◽  
Author(s):  
P. J. Sirois ◽  
G. L. Amidon ◽  
J. H. Meyer ◽  
J. Doty ◽  
J. B. Dressman
Keyword(s):  
Particle Size ◽  
Gastric Emptying ◽  
Gravitational Constant ◽  
Particle Density ◽  
Particle Diameter ◽  
High Viscosity ◽  
Fluid Viscosity ◽  
Fluid Flow Rate ◽  
Hydrodynamic Correlation ◽  
Viscosity Polymer

The influence of particle size, particle density, fluid viscosity, and fluid flow rate on the gastric emptying of nondigestible solids was investigated in five dogs with chronically placed fistulas. Six hundred and fifty particles of 13 different size and density combinations were administered simultaneously with 500 ml of either normal saline or low-, medium-, or high-viscosity polymer solutions. The canine stomach was found to discriminate between these solids on the basis of size and density at all levels of viscosity above saline. The observed patterns of emptying are consistent with the hypothesis that gastric emptying of nondigestible solids is governed in part by hydrodynamics and correlate well with the gastric-emptying coefficient (GEC), a dimensionless grouping of variables that takes the form GEC = (Dpy/Dp) [g(rho f - rho p)Dp2]/[eta (nu)] where [g(rho f - rho p)] is particle buoyancy consisting of fluid (rho f) and particle (rho p) densities and g, the gravitational constant; (Dp) is the particle diameter, (Dpy) the estimated pyloric diameter, eta the fluid viscosity, and (nu) the average linear velocity of fluid exiting the stomach.

Molecular Rotors as Reporters for Viscosity of Solutions of Collagen Like Peptides

2021 ◽  
Author(s):  
Christopher McTiernan ◽  
Matias Zuñiga-Bustos ◽  
Roberto Rosales-Rojas ◽  
Pablo Barrias ◽  
May Griffith ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorometric Assay ◽  
Molecular Rotors ◽  
Molecular Rotor

We have studied the suitability of using a molecular rotor-based steady-state fluorometric assay for evaluating changes in both the conformation and the viscosity of collagen-like peptide solutions. Our results indicate...

Solution viscosity regulates chondrocyte proliferation and phenotype during 3D culture

2019 ◽  
Vol 7 (48) ◽  
pp. 7713-7722 ◽  
Author(s):  
Kyubae Lee ◽  
Yazhou Chen ◽  
Xiaomeng Li ◽  
Yongtao Wang ◽  
Naoki Kawazoe ◽  
...  
Keyword(s):  
3D Culture ◽  
High Viscosity ◽  
Solution Viscosity ◽  
Cell Phenotype ◽  
Gelatin Solution ◽  
Chondrocyte Proliferation ◽  
Low Viscosity ◽  
System Solution ◽  
Hydrogel System

Chondrocytes are cultured in a 3D biphasic gelatin solution/hydrogel system. Solution viscosity affects chondrocyte functions. High viscosity is more beneficial for cell phenotype maintenance, while low viscosity is more beneficial for proliferation.

CFD Simulation of the Particle Dispersion Behavior and Mass Transfer–Reaction Kinetics in non-Newton Fluid with High Viscosity

2019 ◽  
Vol 17 (7) ◽  
Author(s):  
Le Xie ◽  
Qi-An Wang ◽  
Xian-Jin Luo ◽  
Zheng-Hong Luo
Keyword(s):  
Mass Transfer ◽  
Reaction Kinetics ◽  
Transfer Reaction ◽  
High Viscosity ◽  
Particle Dispersion ◽  
Agitation Speed ◽  
Fluid Viscosity ◽  
Condensation Polymerization ◽  
Cross Model ◽  
Dispersion Behavior

Abstract Solid particle dispersion and chemical reactions in high-viscosity non-Newtonian fluid are commonly encountered in polymerization systems. In this study, an interphase mass transfer model and a finite-rate/eddy-dissipation formulation were integrated into a computational fluid dynamics model to simulate the dispersion behavior of particles and the mass transfer–reaction kinetics in a condensation polymerization-stirred tank reactor. Turbulence fields were obtained using the standard k–ε model and employed to calculate the mixing rate. Cross model was used to characterize the rheological property of the non-Newton fluid. The proposed model was first validated by experimental data in terms of input power. Then, several key operating variables (i.e. agitation speed, viscosity, and particle size) were investigated to evaluate the dispersive mixing performance of the stirred vessel. Simulation showed that a high agitation speed and a low fluid viscosity favored particle dispersions. This study provided useful guidelines for industrial-scale high-viscosity polymerization reactors.

Plasma viscosity regulates capillary perfusion during extreme hemodilution in hamster skinfold model

1998 ◽  
Vol 275 (6) ◽  
pp. H2170-H2180 ◽  
Author(s):  
Amy G. Tsai ◽  
Barbara Friesenecker ◽  
Michael McCarthy ◽  
Hiromi Sakai ◽  
Marcos Intaglietta
Keyword(s):  
Molecular Weight ◽  
Shear Stress ◽  
Capillary Density ◽  
High Viscosity ◽  
Plasma Viscosity ◽  
Capillary Perfusion ◽  
Low Viscosity ◽  
Arterial Blood ◽  
Wall Shear ◽  
Stress Dependent

Effect of increasing blood viscosity during extreme hemodilution on capillary perfusion and tissue oxygenation was investigated in the awake hamster skinfold model. Two isovolemic hemodilution steps were performed with 6% Dextran 70 [molecular weight (MW) = 70,000] until systemic hematocrit (Hct) was reduced by 65%. A third step reduced Hct by 75% and was performed with the same solution [low viscosity (LV)] or a high-molecular-weight 6% Dextran 500 solution [MW = 500,000, high viscosity (HV)]. Final plasma viscosities were 1.4 and 2.2 cP (baseline of 1.2 cP). Hct was reduced to 11.2 ± 1.1% from 46.2 ± 1.5% for LV and to 11.9 ± 0.7% from 47.3 ± 2.1% for HV. HV produced a greater mean arterial blood pressure than LV. Functional capillary density (FCD) was substantially higher after HV (85 ± 12%) vs. LV (38 ± 30%) vs. baseline (100%).[Formula: see text] levels measured with Pd-porphyrin phosphorescence microscopy were not statistically changed from baseline until after the third hemodilution step. Wall shear rate (WSR) decreased in arterioles and venules after LV and only in arterioles after HV. Wall shear stress (WSR × plasma viscosity) was substantially higher after HV vs. LV. Increased mean arterial pressure and shear stress-dependent release of endothelium-derived relaxing factor are possible mechanisms that improved arteriolar and venular blood flow and FCD after HV vs. LV exchange protocols.

NEWTONIAN AND NON-NEWTONIAN BLOOD FLOW AT A 90∘-BIFURCATION OF THE CEREBRAL ARTERY: A COMPARATIVE STUDY OF FLUID VISCOSITY MODELS

2018 ◽  
Vol 18 (05) ◽  
pp. 1850043 ◽  
Author(s):  
S. V. FROLOV ◽  
S. V. SINDEEV ◽  
D. LIEPSCH ◽  
A. BALASSO ◽  
P. ARNOLD ◽  
...  
Keyword(s):  
Blood Flow ◽  
Newtonian Fluid ◽  
Flow Rate ◽  
Fluid Model ◽  
Cerebral Arteries ◽  
High Viscosity ◽  
Flow Rate Ratio ◽  
Parent Artery ◽  
Fluid Viscosity ◽  
Recirculating Flow

The majority of numerical simulations assumes blood as a Newtonian fluid due to an underestimation of the effect of non-Newtonian blood behavior on hemodynamics in the cerebral arteries. In the present study, we evaluated the effect of non-Newtonian blood properties on hemodynamics in the idealized 90[Formula: see text]-bifurcation model, using Newtonian and non-Newtonian fluids and different flow rate ratios between the parent artery and its branch. The proposed Local viscosity model was employed for high-precision representation of blood viscosity changes. The highest velocity differences were observed at zones with slow recirculating flow. During the systolic peak the average difference was 17–22%, whereas at the end of diastole the difference increased to 27–60% depending on the flow rate ratio. The main changes in the viscosity distribution were observed distal to the flow separation point, where the non-Newtonian fluid model produced 2.5 times higher viscosity. A presence of such high viscosity region substantially affected the size of the flow recirculation zone. The observed differences showed that non-Newtonian blood behavior had a significant effect on hemodynamic parameters and should be considered in the future studies of blood flow in cerebral arteries.

Influence of Hydrodynamic Fluid Pressure and Shoe Tread Depth on Available Coefficient of Friction

2012 ◽  
Author(s):  
Gurjeet Singh ◽  
Kurt Beschorner
Keyword(s):  
Coefficient Of Friction ◽  
Hydrodynamic Lubrication ◽  
Fluid Pressure ◽  
Hydrodynamic Pressure ◽  
High Viscosity ◽  
Health Concern ◽  
Fluid Viscosity ◽  
Low Viscosity ◽  
The Coefficient Of Friction ◽  
Lubrication Mechanisms

Slip and fall accidents are a major occupational health concern. Identifying the lubrication mechanisms affecting shoe-floor-contaminant friction under biofidelic (testing conditions that mimic human slipping) conditions is critical to identifying unsafe surfaces and designing a slip-resistant work environment. The purpose of this study is to measure the effects of varying tread design, tread depth and fluid viscosity on underfoot hydrodynamic pressure, the load supported by the fluid (i.e. load carrying capacity), and the coefficient of friction (COF) during a simulated slip. A single vinyl floor material and two shoe types (work shoe and sportswear shoe) with three different tread depths (no tread, half tread and full tread) were tested under two lubrication conditions: 1) 90% glycerol and 10% water (219 cP) and 2) 1.5% Detergent-98.5% (1.8cP) water solutions. Hydrodynamic pressures were measured with a fluid pressure sensor embedded in the floor and a forceplate was used to measure the friction and normal forces used to calculate coefficient of friction. The study showed that hydrodynamic pressure developed when high viscosity fluids were combined with no tread and resulted in a major reduction of COF (0.005). Peak hydrodynamic pressures (and load supported by the fluid) for the no tread-high viscous conditions were 234 kPa (200.5 N) and 87.63 kPa (113.3 N) for the work and sportswear shoe, respectively. Hydrodynamic pressures were negligible when at least half the tread was present or when a low viscosity fluid was used despite the fact that many of these conditions also resulted in dangerously low COF values. The study suggests that hydrodynamic lubrication is only relevant when high viscous fluids are combined with little or no tread and that other lubrication mechanisms besides hydrodynamic effects are relevant to slipping like boundary lubrication.

scholarly journals Numerical Study on Deformation and Interior Flow of a Droplet Suspended in Viscous Liquid under Steady Electric Fields

2014 ◽  
Vol 6 ◽  
pp. 532797 ◽  
Author(s):  
Zhentao Wang ◽  
Qingming Dong ◽  
Yonghui Zhang ◽  
Junfeng Wang ◽  
Jianlong Wen
Keyword(s):  
Electric Fields ◽  
Numerical Study ◽  
Internal Flow ◽  
High Viscosity ◽  
Numerical Results ◽  
Experimental Results ◽  
Fluid Viscosity ◽  
Single Droplet ◽  
Deformation Velocity ◽  
Interior Flow

A model based on the volume of fluid (VOF) method and leaky dielectric theory is established to predict the deformation and internal flow of the droplet suspended in another vicious fluid under the influence of the electric field. Through coupling with hydrodynamics and electrostatics, the rate of deformation and internal flow of the single droplet are simulated and obtained under the different operating parameters. The calculated results show that the direction of deformation and internal flow depends on the physical properties of fluids. The numerical results are compared with Taylor's theory and experimental results by Torza et al. When the rate of deformation is small, the numerical results are consistent with theory and experimental results, and when the rate is large the numerical results are consistent with experimental results but are different from Taylor's theory. In addition, fluid viscosity hardly affects the deformation rate and mainly dominates the deformation velocity. For high viscosity droplet spends more time to attain the steady state. The conductivity ratio and permittivity ratio of two different liquids affect the direction of deformation. When fluid electric properties change, the charge distribution at the interface is various, which leads to the droplet different deformation shapes.

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