scholarly journals Sexual dimorphism in intestinal absorption and lymphatic transport of dietary lipids

2021 ◽  
Author(s):  
Min Liu ◽  
Ling Shen ◽  
Qing Yang ◽  
Andromeda M. Nauli ◽  
Madison Bingamon ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Intestinal Absorption ◽  
Dietary Lipids ◽  
Lymphatic Transport

Effect of soy protein and casein intake on intestinal absorption and lymphatic transport of cholesterol and oleic acid

1984 ◽  
Vol 40 (6) ◽  
pp. 1156-1164 ◽  
Author(s):  
G V Vahouny ◽  
W Chalcarz ◽  
S Satchithanandam ◽  
I Adamson ◽  
D M Klurfeld ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Intestinal Absorption ◽  
Soy Protein ◽  
Lymphatic Transport

Triacylglycerol and cholesterol transport during absorption of glycerol trioleate vs. glycerol trielaidate

1996 ◽  
Vol 270 (2) ◽  
pp. G268-G276
Author(s):  
T. J. Kalogeris ◽  
L. Gray ◽  
Y. Y. Yeh ◽  
P. Tso
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Intestinal Absorption ◽  
Acid Composition ◽  
Trans Fatty Acid ◽  
Cholesterol Absorption ◽  
Lymphatic Transport ◽  
Cholesterol Transport ◽  
Lipid Emulsions ◽  
Intestinal Lymph

We used conscious, chronic lymph-fistula rats to compare intestinal lymphatic transport of glycerol trioleate (TO) vs. glycerol trielaidate (TE) and to determine the effect of TO vs. TE on absorption and transport of cholesterol. Rats were implanted with intestinal lymph fistulas and duodenal cannulas and then given intraduodenal infusions of lipid emulsions containing purified TO or TE (40 mumol/h) and cholesterol (7.8 mumol/h + 2 microCi [14C]cholesterol). Lymph samples were collected at 0, 2, 4, 5, 6, 7, and 8 h after the start of lipid infusion. Lymphatic output and luminal and gut wall recovery of radioactive lipid at 8 h were quantified. Triacylglycerol (TG) fatty acid isomers did not affect lymphatic output of TG; lymph TG fatty acid composition and output reflected infusate composition. Lymphatic output of cholesterol (mass and radioactivity) did not differ between groups; luminal and gut wall recovery of [14C]cholesterol was also similar between groups. Similar lymphatic transport of TG and cholesterol between triolein- and trielaidin-infused rats was maintained for up to 16 h after the cessation of an infused lipid load. These results indicate that TO and TE are transported into lymph similarly, and that cholesterol absorption and transport are similar irrespective of whether TO or TE is the TG source. The data suggest that trans fatty acid-induced hypercholesterolemia is not due to altered intestinal absorption and transport of cholesterol.

scholarly journals Apolipoprotein A-V deficiency enhances chylomicron production in lymph fistula mice

2015 ◽  
Vol 308 (7) ◽  
pp. G634-G642 ◽  
Author(s):  
Linda S. Zhang ◽  
Min Xu ◽  
Qing Yang ◽  
Robert O. Ryan ◽  
Philip Howles ◽  
...  
Keyword(s):  
Independent Predictor ◽  
Dietary Lipids ◽  
Intestinal Lumen ◽  
Lymphatic Transport ◽  
Wild Type ◽  
Plasma Triglycerides ◽  
Apolipoprotein A ◽  
Threefold Increase ◽  
Postprandial Hypertriglyceridemia ◽  
Steady State Absorption

Apolipoprotein A-V (apoA-V), a liver-synthesized apolipoprotein discovered in 2001, strongly modulates fasting plasma triglycerides (TG). Little is reported on the effect of apoA-V on postprandial plasma TG, an independent predictor for atherosclerosis. Overexpressing apoA-V in mice suppresses postprandial TG, but mechanisms focus on increased lipolysis or clearance of remnant particles. Unknown is whether apoA-V suppresses the absorption of dietary lipids by the gut. This study examines how apoA-V deficiency affects the steady-state absorption and lymphatic transport of dietary lipids in chow-fed mice. Using apoA-V knockout (KO, n = 8) and wild-type (WT, n = 8) lymph fistula mice, we analyzed the uptake and lymphatic transport of lipids during a continuous infusion of an emulsion containing [3H]triolein and [14C]cholesterol. ApoA-V KO mice showed a twofold increase in3H ( P < 0.001) and a threefold increase in14C ( P < 0.001) transport into the lymph compared with WT. The increased lymphatic transport was accompanied by a twofold reduction ( P < 0.05) in mucosal3H, suggesting that apoA-V KO mice more rapidly secreted [3H]TG out of the mucosa into the lymph. ApoA-V KO mice also produced chylomicrons more rapidly than WT ( P < 0.05), as measured by the transit time of [14C]oleic acid from the intestinal lumen to lymph. Interestingly, apoA-V KO mice produced a steadily increasing number of chylomicron particles over time, as measured by lymphatic apoB output. The data suggest that apoA-V suppresses the production of chylomicrons, playing a previously unknown role in lipid metabolism that may contribute to the postprandial hypertriglyceridemia associated with apoA-V deficiency.

scholarly journals Lymphatic absorption of fatty acids and cholesterol in the neonatal rat

2000 ◽  
Vol 279 (2) ◽  
pp. G325-G331 ◽  
Author(s):  
Looi C. Ee ◽  
Shuqin Zheng ◽  
Lili Yao ◽  
Patrick Tso
Keyword(s):  
Fatty Acids ◽  
Postnatal Growth ◽  
Dietary Lipids ◽  
Neonatal Rat ◽  
Lymphatic Transport ◽  
Sprague Dawley ◽  
Lymphatic Absorption ◽  
Suckling Rats ◽  
Thin Layer Chromatographic Analysis ◽  
Fat Diets

High-fat diets are essential in suckling animals to ensure adequate calories for postnatal growth, but their lymphatic transport of dietary lipids has not been characterized. We established a lymph fistula model in suckling rats to quantify intestinal uptake and lymphatic transport of dietary lipids and analyzed lipoprotein fractions. Suckling 19-day-old Sprague-Dawley rats had their mesenteric lymph ducts cannulated and gastroduodenal tubes inserted. After overnight recovery, [3H]triolein and [14C]cholesterol were infused for 6 h. Of the total dose, only 38% of triolein and 24% of cholesterol were transported in the lymph of suckling rats. Analyses of residual luminal contents and intestinal mucosal homogenate showed neither reduced absorption nor delayed mucosal processing of ingested lipids to be the cause. Thin-layer chromatographic analysis of radioactive mucosal lipids, however, showed a predominance of free fatty acids (60%) and free cholesterol (67%), implying impaired esterification capacity in these animals. We speculate that this reduced esterification allows for portal transport or direct enterocyte metabolism of dietary lipids.

scholarly journals Monosodium glutamate inhibits the lymphatic transport of lipids in the rat

2016 ◽  
Vol 311 (4) ◽  
pp. G648-G654 ◽  
Author(s):  
Alison B. Kohan ◽  
Qing Yang ◽  
Min Xu ◽  
Dana Lee ◽  
Patrick Tso
Keyword(s):  
Continuous Infusion ◽  
Intestinal Mucosa ◽  
Lipid Emulsion ◽  
Monosodium Glutamate ◽  
Dietary Lipids ◽  
Intestinal Lumen ◽  
Lymphatic Transport ◽  
Gastrointestinal Physiology ◽  
Cholesterol Secretion

It is not well understood how monosodium glutamate (MSG) affects gastrointestinal physiology, especially regarding the absorption and the subsequent transport of dietary lipids into lymph. Thus far, there is little information about how the ingestion of MSG affects the lipid lipolysis, uptake, intracellular esterification, and formation and secretion of chylomicrons. Using lymph fistula rats treated with the infusion of a 2% MSG solution before a continuous infusion of triglyceride, we show that MSG causes a significant decrease in both triglyceride and cholesterol secretion into lymph. Intriguingly, the diminished lymphatic transport of triglyceride and cholesterol was not caused by an accumulation of these labeled lipids in the intestinal lumen or in the intestinal mucosa. Rather, it is a result of increased portal transport in the animals fed acutely the lipid plus 2% MSG in the lipid emulsion. This is a first demonstration of MSG on intestinal lymphatic transport of lipids.

Intestinal absorption and lymphatic transport of peroxidized lipids in rats: effect of exogenous GSH

1992 ◽  
Vol 263 (5) ◽  
pp. G665-G672 ◽  
Author(s):  
T. Y. Aw ◽  
M. W. Williams
Keyword(s):  
Steady State ◽  
Intestinal Absorption ◽  
Lipid Hydroperoxide ◽  
Buthionine Sulfoximine ◽  
Lymphatic Transport ◽  
Antioxidant Systems ◽  
Lipid Hydroperoxides ◽  
Intestinal Metabolism ◽  
Proximal Intestine

We previously found that mucosal glutathione (GSH) plays an important role in the intestinal metabolism of luminal peroxidized lipids [T. Y. Aw, M. W. Williams, and L. Gray. Am. J. Physiol. 262 (Gastrointest. Liver Physiol. 25): G99-G106, 1992]. To determine the effects of exogenous GSH on lipid hydroperoxide elimination under conditions in which mucosal GSH was initially depleted with buthionine sulfoximine (BSO), we infused peroxidized lipid solutions without or with GSH into the proximal intestine of rats and monitored the steady-state output of hydroperoxides in lymph and recovery of luminal hydroperoxides. GSH supplementation in BSO-treated rats resulted in a concentration-dependent attenuation of lymphatic output of peroxidized lipids that was correlated with increases in mucosal GSH. Compared with BSO control, the luminal lipid hydroperoxide contents were significantly lower in GSH-supplemented rats, consistent with enhanced elimination of peroxidized lipids by exogenous GSH. The effect of GSH was ameliorated by the inhibitors of GSH uptake, suggesting that the uptake of GSH is required for promotion of intestinal removal of luminal hydroperoxides. Other thiols, either at comparable or higher concentrations than GSH, were without significant effects on lymphatic transport or luminal recovery of lipid hydroperoxides, indicating that these thiols are poor substitutes for GSH. Overall, the data are consistent with exogenous GSH being a source for cellular reduction of peroxidized lipids. Results from these studies could directly impact on future consideration of therapeutic means to increase cellular antioxidant systems to promote intestinal hydroperoxide detoxication.

Effect of abdominal surgery on the intestinal absorption of lipophilic drugs: possible role of the lymphatic transport

2009 ◽  
Vol 153 (6) ◽  
pp. 296-300 ◽  
Author(s):  
Pavel Gershkovich ◽  
Constantin Itin ◽  
Avihai Yacovan ◽  
Shimon Amselem ◽  
Amnon Hoffman
Keyword(s):  
Abdominal Surgery ◽  
Intestinal Absorption ◽  
Lymphatic Transport ◽  
Lipophilic Drugs
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