Mapping and molecular characterization of five HMG1-related DNA sequences

1998 ◽  
Vol 83 (1-2) ◽  
pp. 124-129 ◽  
Author(s):  
P. Rogalla ◽  
Z. Borda ◽  
K. Meyer-Bolte ◽  
K.H. Tran ◽  
S. Hauke ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences

Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Paraná River, Brazil

2015 ◽  
Vol 90 (6) ◽  
pp. 693-697 ◽  
Author(s):  
L.S. Gasques ◽  
R.J. Graça ◽  
S.M.A.P. Prioli ◽  
R.M. Takemoto ◽  
A.J. Prioli
Keyword(s):  
Cryptic Species ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Genetic Data ◽  
Flood Plain ◽  
Fish Parasites ◽  
Coi Gene ◽  
Coi Sequences

AbstractUrocleidoides ectoparasites are mainly found on fish of the neotropical regions. Although molecular research on monogeneans is available, no genetic data exist characterizing species in the Urocleidoides genus. Some DNA sequences have been efficacious in systematic studies and in the reconstruction of phylogenies of fish parasites. Relevant roles have been given to the sequence of the mitochondrial gene of cytochrome c oxidase I (COI). This study characterized COI sequences of the parasites Urocleidoides malabaricusi and U. cuiabai in trahira fish Hoplias aff. malabaricus of the flood plain of the Upper River Paraná, Brazil. The two species under analysis were distinguished by sequencing and analysing a 420-bp fragment of the COI gene, which suggested the existence of the cryptic species U. malabaricusi.

scholarly journals Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences

2016 ◽  
Vol 8 (2) ◽  
pp. 185
Author(s):  
Nia Kurniawaty ◽  
Purnama Hidayat ◽  
Aunu Rauf
Keyword(s):  
Pest Management ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Biology Education ◽  
Morphological Data ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Vegetable Crops ◽  
Total Extraction

<p>Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of  Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps  DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis.  PCR programme was succesfully to amplified mt<em>COI</em> gene fragment at 710 bp. The length of mt<em>COI </em>gene of <em>Gynaikothrips uzeli, Haplothrips ganglbaueri</em>, and <em>Pseudophilothrips ichini</em> were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mt<em>COI </em>gene mitochondrial had successfully supported the morphological data. </p><p><strong>How to Cite</strong></p><p>Kurniawaty, N., Hidayat, P. &amp; Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 185-192.</p>

Molecular characterization of a Mycobacterium species in non-native poeciliids in Hawaii using DNA sequences

2006 ◽  
Vol 29 (3) ◽  
pp. 181-185 ◽  
Author(s):  
M J Poort ◽  
C M Whipps ◽  
V G Watral ◽  
W F Font ◽  
M L Kent
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences ◽  
Mycobacterium Species

scholarly journals Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

2005 ◽  
Vol 67 (11) ◽  
pp. 1115-1118 ◽  
Author(s):  
Tadashi ITAGAKI ◽  
Masayuki KIKAWA ◽  
Kunio TERASAKI ◽  
Toshiyuki SHIBAHARA ◽  
Koichi FUKUDA
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences

scholarly journals Morphologycal and Molecular Partial Histone-H3 Characterization of Bintan Sea Snail Gonggong (Strombus sp.) as a Species Validation

2019 ◽  
Vol 26 (2) ◽  
pp. 56
Author(s):  
Lily Viruly ◽  
Nuri Andarwulan ◽  
Maggy T. Suhartono ◽  
Mala Nurilmala
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Shell Length ◽  
Bioinformatics Analysis ◽  
Economic Value ◽  
Histone H3 ◽  
Shell Width

Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. It is a favorite seafood item in Riau Islands Province, and is high economic value but not known widely yet. Until now, sea snail gonggong has been highly exploited but the research on this snail is very limited. The aim of this study was to morphology and molecular characterization of Bintan gonggong snail (Strombus sp.) as a species validation. Bintan gonggong snail included thick-shelled gonggong and thin-shelled gonggong. Morphology identifcation of species Bintan gonggong snail was based on morphometric variability. Molecular identifcation used partial Histone-H3, MEGA version 6.06, and bioinformatics analysis. The result showed that the morphological identifcation of thick-shelled and thin-shelled gonggong based on shell width, the lip thickness, and total weight signifcantly different, but other variables (i.e shell length, shell depth, aperture length, and gonggong weight) were not signifcantly different (p<0.05). Resulted of a molecular identifcation with phylogenetic analysis that the thin-shelled and thick-shelled Bintan gonggong snails were 1 species and a genetic distance of 1%. They were not species Strombus canarium, Strombus vitatus, and Strombus epidromis. Bintan gonggong snails were Strombus turturella (Leavistrombus turturella). DNA sequences of Bintan gonggong have been registered in Gen-Bank with registration numbers MH348131 (thinshelled gonggong) and MH348132 (thick-shelled gonggong).

scholarly journals Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq

2018 ◽  
pp. 177-182
Author(s):  
Abdullah Ahmed Hama ◽  
Rostam Hama Zorab ◽  
Fatima Mohammed Ali ◽  
Awaz M. Salih ◽  
Amer Abdullah Hassan
Keyword(s):  
Hydatid Cyst ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Prevalence Rate ◽  
Accession Number ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Cysticercus Tenuicollis ◽  
Nucleotide Sequence Alignment

Cysticercus tenuicollis (C. tenuicollis) is the larval stage infection of Taenia hydatigena, a common tapeworm of dogs and other Canidae, which has a wide range of intermediate hosts including sheep, goat, cattle, deer, camel, horse, human and other wild ruminants, the disease spreads through a contaminated water, soil and food with feces of infected dogs or other carnivores, T. hydatigena lives in the intestinal of the definitive host (carnivores) and excretes a huge number of eggs with feces daily. The present study conducted to determine the prevalence rate and molecular characterization of C. tenuicollis among sheep and goats in Sulaimani province. A total of 14088 slaughtered animals were inspected postmortem from the new Sulaimani abattoir comprise, which involves 13395 sheep and 693 goats. The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study,  The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. The prevalence rate of C. tenuicollis was 2.63% in sheep and 2.58% in goats. This result shows no significant differences of C. tenuicollis between sheep and goats (p>0.05). The nucleotide sequence alignment of cytochrome oxidase subunit 1 (CO 1) gene revealed that the amplified DNA fragment is belong to Taenia hydatigena and Echinococcus granulosus and the nucleotide sequences of T. hydatigena deposited in GenBank under accession number (MH638348). This finding concludes that the amplification of mt-CO1 gene cannot be depended on discriminate hydatid cyst and C. tenuicollis while the partial DNA sequences of mt-CO1 gene are significantly valuable to differentiate C. tenuicollis from hydatid cyst, which is completely different in the pathology and control.

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