scholarly journals Characterization of porcine endogenous retrovirus expression in neonatal and adult pig pancreatic islets

2017 ◽  
Vol 24 (4) ◽  
pp. e12311 ◽  
Author(s):  
Nizar I. Mourad ◽  
Claire Crossan ◽  
Victoria Cruikshank ◽  
Linda Scobie ◽  
Pierre Gianello
Keyword(s):  
Pancreatic Islets ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig

Virus Genes ◽  
2009 ◽  
Vol 39 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Na Young Kim ◽  
Donghee Lee ◽  
Jungeun Lee ◽  
Eung Woo Park ◽  
Woon-Won Jung ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Domestic Pig ◽  
Molecular Clone ◽  
Porcine Endogenous Retrovirus ◽  
Class B

scholarly journals Expression and characterization of a recombinant novel reverse transcriptase of a porcine endogenous retrovirus

Virology ◽  
2003 ◽  
Vol 307 (2) ◽  
pp. 341-357 ◽  
Author(s):  
Orna Avidan ◽  
Shoshana Loya ◽  
Ralf R Tönjes ◽  
Ziv Sevilya ◽  
Amnon Hizi
Keyword(s):  
Reverse Transcriptase ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

scholarly journals Isolation and Characterization of an Infectious Replication-Competent Molecular Clone of Ecotropic Porcine Endogenous Retrovirus Class C

2006 ◽  
Vol 80 (20) ◽  
pp. 10258-10261 ◽  
Author(s):  
Thomas Preuss ◽  
Nicole Fischer ◽  
Klaus Boller ◽  
Ralf R. Tönjes
Keyword(s):  
T Cells ◽  
Genomic Library ◽  
Endogenous Retrovirus ◽  
Endogenous Retroviruses ◽  
Miniature Swine ◽  
Infectious Risk ◽  
Isolation And Characterization ◽  
Porcine Endogenous Retrovirus ◽  
Porcine Endogenous Retroviruses

ABSTRACT Xenotransplantation of pig organs is complicated by the existence of polytropic replication-competent porcine endogenous retroviruses (PERV) capable of infecting human cells. The potential for recombination between ecotropic PERV-C and human-tropic PERV-A and PERV-B adds another level of infectious risk. Proviral PERV-C were characterized in MAX-T cells derived from d/d haplotype miniature swine. Three proviruses were cloned from a genomic library. Clone PERV-C(1312) generated infectious particles after transfection into porcine ST-IOWA cells. Electron microscopy revealed the same morphologies of virions in MAX-T cells and in ST-IOWA cells infected with cell-free PERV-C(1312) particles, indicating that MAX-T cells harbor one functional PERV-C provirus.

scholarly journals Characterization of Porcine Endogenous Retrovirus γ pro-pol Nucleotide Sequences

2002 ◽  
Vol 76 (22) ◽  
pp. 11738-11743 ◽  
Author(s):  
Nikolai Klymiuk ◽  
Mathias Müller ◽  
Gottfried Brem ◽  
Bernhard Aigner
Keyword(s):  
Endogenous Retrovirus ◽  
Open Reading Frames ◽  
The Novel ◽  
Infectious Risk ◽  
Porcine Endogenous Retrovirus ◽  
Pig Genome ◽  
Reading Frames ◽  
Selection Of

ABSTRACT Endogenous retroviral sequences in the pig genome (PERV) represent a potential infectious risk in xenotransplantation. All known infectious PERV have been asssigned to the PERV γ1 family, consisting of the subfamilies A, B, and C. The aim of the study was the concise examination of PERV γ by the analysis of the retroviral pro-pol sequences. The analysis of 52 pro-pol clones amplified in this study revealed eight PERV γ families. In addition to four already-described families (γ1, γ4, γ5, γ6), four novel families (γ7, γ8, γ9, γ10) were identified. Quantitative analysis of the novel PERV γ sequences in selected breeds revealed variations in the endogenous retroviral load. Open reading frames (ORF) in the amplified proviral fragment were only found for PERV γ1. In addition, novel ORF-containing PERV γ1 clones consisting of hybrid sequences were revealed. Sequence comparison from published full-length PERV γ1 clones of the PERV subfamilies A, B, and C resulted in a lack of strict correlation of the classification of pro-pol and env. The results indicated the occurrence of causative recombination events between retroviral genomes. Thus, our study on PERV γ provides new data for the evaluation and selection of pigs intended to be used in xenotransplantation.

scholarly journals PCR-based cloning and immunocytological titration of infectious porcine endogenous retrovirus subgroup A and B

2002 ◽  
Vol 83 (9) ◽  
pp. 2231-2240 ◽  
Author(s):  
Birke Bartosch ◽  
Robin A. Weiss ◽  
Yasuhiro Takeuchi
Keyword(s):  
Restriction Site ◽  
Current Method ◽  
Endogenous Retrovirus ◽  
Endogenous Retroviruses ◽  
Porcine Endogenous Retrovirus ◽  
293 Cells ◽  
Cloning Technique ◽  
Unique Restriction ◽  
Human Xenotransplantation

Two pig endogenous retroviruses (PERV), PERV-A and -B, productively infect human cells and are therefore considered to constitute a potential risk in pig-to-human xenotransplantation. A PCR-based cloning technique to isolate infectious PERV proviruses was established. Overlapping 3′ half and 5′ halves of PERV proviral genomes were amplified using DNA extracted from human 293 cells infected with PERV-A or -B. These clones were fused at a unique restriction site in the overlapping region and tested for their infectivity. Representative constructs possessed the same infectious properties as their parent isolates. We also developed a polyclonal anti-PERV serum by using recombinant PERV capsid protein derived from one of the infectious constructs as immunogen and established an immunocytological method for detection and titration of PERV infection. This detection method proved to be more sensitive than the current method of choice (transfer of MLV-lacZ vectors) for infectivity assessment of PERV. These findings should be considered for future characterization of PERV isolates.

scholarly journals Characterization of porcine endogenous retrovirus particles released by the CRISPR/Cas9 inactivated cell line PK15 clone 15

2019 ◽  
Vol 27 (2) ◽  
Author(s):  
Antonia W. Godehardt ◽  
Nicole Fischer ◽  
Paula Rauch ◽  
Barbara Gulich ◽  
Klaus Boller ◽  
...  
Keyword(s):  
Cell Line ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

Characterization of Clones of Human Cell Line Infected with Porcine Endogenous Retrovirus (PERV) from Porcine Cell Line, PK-15

2009 ◽  
Vol 41 (1) ◽  
pp. 1 ◽  
Author(s):  
Jung Heon Kim ◽  
Eun young Choi ◽  
Eun-Suk Jung ◽  
Yejin Kwon ◽  
Dong Suk Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus ◽  
Porcine Cell
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