scholarly journals Characterization of porcine endogenous retrovirus particles released by the CRISPR/Cas9 inactivated cell line PK15 clone 15

2019 ◽  
Vol 27 (2) ◽  
Author(s):  
Antonia W. Godehardt ◽  
Nicole Fischer ◽  
Paula Rauch ◽  
Barbara Gulich ◽  
Klaus Boller ◽  
...  
Keyword(s):  
Cell Line ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

Characterization of Clones of Human Cell Line Infected with Porcine Endogenous Retrovirus (PERV) from Porcine Cell Line, PK-15

2009 ◽  
Vol 41 (1) ◽  
pp. 1 ◽  
Author(s):  
Jung Heon Kim ◽  
Eun young Choi ◽  
Eun-Suk Jung ◽  
Yejin Kwon ◽  
Dong Suk Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus ◽  
Porcine Cell

scholarly journals Extended Analysis of the In Vitro Tropism of Porcine Endogenous Retrovirus

2000 ◽  
Vol 74 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Carolyn A. Wilson ◽  
Susan Wong ◽  
Matthew VanBrocklin ◽  
Mark J. Federspiel
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Line ◽  
Endogenous Retrovirus ◽  
Human Cells ◽  
Productive Infection ◽  
Porcine Endogenous Retrovirus ◽  
Productive Replication

ABSTRACT We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082–3087, 1998). We now extend that analysis to show that additional passage of isolated virus, named here PERV-NIH, through a human cell line yielded a viral population with a higher titer of infectious virus on human cells than the initial isolate. We show that in a single additional passage on a human cell line, the increase in infectivity for human cells is accounted for by selection against variants carrying pig-tropic envelope sequences (PERV-C) as well as by enrichment for replication-competent genomes. Sequence analysis of the envelope cDNA present in virions demonstrated that the envelope sequence of PERV-NIH is related to but distinct from previously reported PERV envelopes. The in vitro host range of PERV was studied in human primary cells and cell lines, as well as in cell lines from nonhuman primate and other species. This analysis reveals three patterns of susceptibility to infection among these host cells: (i) cells are resistant to infection in our assay; (ii) cells are infected by virus, as viral RNA is detected in the supernatant by reverse transcription-PCR, but the cells are not permissive to productive replication and spread; and (iii) cells are permissive to low-level productive replication. Certain cell lines were permissive for efficient productive infection and spread. These results may prove useful in designing appropriate animal models to assess the in vivo infectivity properties of PERV.

scholarly journals Quantitative Estimation of Porcine Endogenous Retrovirus Release from PK15 Cells

2012 ◽  
Vol 61 (3) ◽  
pp. 211-215 ◽  
Author(s):  
MAGDALENA KIMSA ◽  
BARBARA STRZALKA-MROZIK ◽  
MALGORZATA KIMSA ◽  
JOLANTA ADAMSKA ◽  
JOANNA GOLA ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Cell Line ◽  
Culture Medium ◽  
Quantitative Estimation ◽  
Endogenous Retrovirus ◽  
Time Dependent ◽  
Dependent Manner ◽  
Pk15 Cell ◽  
Porcine Endogenous Retrovirus

The present study focuses on the assessment of porcine endogenous retrovirus (PERV) release from PK15 cells in a time dependent manner. The highest amount of PERV A RNA was detected in PK15 cells after 16 hours of culture. The highest amount of PERV B RNA was detected in PK15 cells after 20 hours. The highest amount of both subtypes RNAs was detected in culture medium after 32 hours of culture. The peaks of PERV reverse transcriptase (RT) activity were detected after 28 h of culture in PK15 cells and after 32 hours in the culture medium. The monitoring of PERV release from PK15 cell line may be useful for the evaluation of PERV replication.

Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig

Virus Genes ◽  
2009 ◽  
Vol 39 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Na Young Kim ◽  
Donghee Lee ◽  
Jungeun Lee ◽  
Eung Woo Park ◽  
Woon-Won Jung ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Domestic Pig ◽  
Molecular Clone ◽  
Porcine Endogenous Retrovirus ◽  
Class B

scholarly journals Expression and characterization of a recombinant novel reverse transcriptase of a porcine endogenous retrovirus

Virology ◽  
2003 ◽  
Vol 307 (2) ◽  
pp. 341-357 ◽  
Author(s):  
Orna Avidan ◽  
Shoshana Loya ◽  
Ralf R Tönjes ◽  
Ziv Sevilya ◽  
Amnon Hizi
Keyword(s):  
Reverse Transcriptase ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

scholarly journals Characterization of porcine endogenous retrovirus expression in neonatal and adult pig pancreatic islets

2017 ◽  
Vol 24 (4) ◽  
pp. e12311 ◽  
Author(s):  
Nizar I. Mourad ◽  
Claire Crossan ◽  
Victoria Cruikshank ◽  
Linda Scobie ◽  
Pierre Gianello
Keyword(s):  
Pancreatic Islets ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

scholarly journals Human CD59 Incorporation into Porcine Endogenous Retrovirus Particles: Implications for the Use of Transgenic Pigs for Xenotransplantation

2002 ◽  
Vol 76 (4) ◽  
pp. 1999-2002 ◽  
Author(s):  
Daniel M. Takefman ◽  
Gregory T. Spear ◽  
Mohammed Saifuddin ◽  
Carolyn A. Wilson
Keyword(s):  
Human Serum ◽  
Cell Line ◽  
Endogenous Retrovirus ◽  
Protective Effects ◽  
Transgenic Pigs ◽  
Complement Control Protein ◽  
Porcine Endogenous Retrovirus ◽  
Human Sera ◽  
Human Cd59 ◽  
Control Protein

ABSTRACT Transgenic pigs have been engineered to express human CD59 (hCD59) in order to suppress hyperacute rejection of xenotransplants in human recipients. In this study, porcine endogenous retrovirus (PERV) was produced in a porcine cell line expressing hCD59 in order to examine the effect of this complement control protein on PERV neutralization by human sera. hCD59 was found to be incorporated into PERV particles produced from engineered ST-IOWA cells. PERV incorporation of hCD59 resulted in a dramatic inhibition of complement-mediated virolysis by human serum. However, incorporation of hCD59 had no effect on neutralization of PERV by human serum, as measured in infectivity assays. Our results suggest that the use of organs from hCD59 transgenic pigs will inhibit complement-mediated virolysis, but will not compromise the protective effects of human sera on the neutralization of PERV particles.

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