Molecular Characterization of the Porcine Endogenous Retrovirus Subclass A and B Envelope Gene From Pigs

2006 ◽  
Vol 38 (9) ◽  
pp. 3066-3069 ◽  
Author(s):  
D. Lee ◽  
J. Lee ◽  
S.J. Uhm ◽  
Y.S. Lee ◽  
M.J. Park ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Endogenous Retrovirus ◽  
Envelope Gene ◽  
Porcine Endogenous Retrovirus

scholarly journals Molecular characterization of the Akvr-1 restriction gene: a defective endogenous retrovirus-borne gene identical to Fv-4r.

1987 ◽  
Vol 61 (2) ◽  
pp. 308-314 ◽  
Author(s):  
S Dandekar ◽  
P Rossitto ◽  
S Pickett ◽  
G Mockli ◽  
H Bradshaw ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Endogenous Retrovirus

Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig

Virus Genes ◽  
2009 ◽  
Vol 39 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Na Young Kim ◽  
Donghee Lee ◽  
Jungeun Lee ◽  
Eung Woo Park ◽  
Woon-Won Jung ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Domestic Pig ◽  
Molecular Clone ◽  
Porcine Endogenous Retrovirus ◽  
Class B

scholarly journals Molecular characterization of full-length MLV-related endogenous retrovirus ChiRV1 from the chicken, Gallus gallus

Virology ◽  
2008 ◽  
Vol 376 (1) ◽  
pp. 199-204 ◽  
Author(s):  
Leonid Borysenko ◽  
Volodymir Stepanets ◽  
Alla V. Rynditch
Keyword(s):  
Molecular Characterization ◽  
Gallus Gallus ◽  
Endogenous Retrovirus ◽  
Full Length

scholarly journals Expression and characterization of a recombinant novel reverse transcriptase of a porcine endogenous retrovirus

Virology ◽  
2003 ◽  
Vol 307 (2) ◽  
pp. 341-357 ◽  
Author(s):  
Orna Avidan ◽  
Shoshana Loya ◽  
Ralf R Tönjes ◽  
Ziv Sevilya ◽  
Amnon Hizi
Keyword(s):  
Reverse Transcriptase ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

scholarly journals Characterization of porcine endogenous retrovirus expression in neonatal and adult pig pancreatic islets

2017 ◽  
Vol 24 (4) ◽  
pp. e12311 ◽  
Author(s):  
Nizar I. Mourad ◽  
Claire Crossan ◽  
Victoria Cruikshank ◽  
Linda Scobie ◽  
Pierre Gianello
Keyword(s):  
Pancreatic Islets ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus

scholarly journals Isolation and Characterization of an Infectious Replication-Competent Molecular Clone of Ecotropic Porcine Endogenous Retrovirus Class C

2006 ◽  
Vol 80 (20) ◽  
pp. 10258-10261 ◽  
Author(s):  
Thomas Preuss ◽  
Nicole Fischer ◽  
Klaus Boller ◽  
Ralf R. Tönjes
Keyword(s):  
T Cells ◽  
Genomic Library ◽  
Endogenous Retrovirus ◽  
Endogenous Retroviruses ◽  
Miniature Swine ◽  
Infectious Risk ◽  
Isolation And Characterization ◽  
Porcine Endogenous Retrovirus ◽  
Porcine Endogenous Retroviruses

ABSTRACT Xenotransplantation of pig organs is complicated by the existence of polytropic replication-competent porcine endogenous retroviruses (PERV) capable of infecting human cells. The potential for recombination between ecotropic PERV-C and human-tropic PERV-A and PERV-B adds another level of infectious risk. Proviral PERV-C were characterized in MAX-T cells derived from d/d haplotype miniature swine. Three proviruses were cloned from a genomic library. Clone PERV-C(1312) generated infectious particles after transfection into porcine ST-IOWA cells. Electron microscopy revealed the same morphologies of virions in MAX-T cells and in ST-IOWA cells infected with cell-free PERV-C(1312) particles, indicating that MAX-T cells harbor one functional PERV-C provirus.

scholarly journals Characterization of Porcine Endogenous Retrovirus γ pro-pol Nucleotide Sequences

2002 ◽  
Vol 76 (22) ◽  
pp. 11738-11743 ◽  
Author(s):  
Nikolai Klymiuk ◽  
Mathias Müller ◽  
Gottfried Brem ◽  
Bernhard Aigner
Keyword(s):  
Endogenous Retrovirus ◽  
Open Reading Frames ◽  
The Novel ◽  
Infectious Risk ◽  
Porcine Endogenous Retrovirus ◽  
Pig Genome ◽  
Reading Frames ◽  
Selection Of

ABSTRACT Endogenous retroviral sequences in the pig genome (PERV) represent a potential infectious risk in xenotransplantation. All known infectious PERV have been asssigned to the PERV γ1 family, consisting of the subfamilies A, B, and C. The aim of the study was the concise examination of PERV γ by the analysis of the retroviral pro-pol sequences. The analysis of 52 pro-pol clones amplified in this study revealed eight PERV γ families. In addition to four already-described families (γ1, γ4, γ5, γ6), four novel families (γ7, γ8, γ9, γ10) were identified. Quantitative analysis of the novel PERV γ sequences in selected breeds revealed variations in the endogenous retroviral load. Open reading frames (ORF) in the amplified proviral fragment were only found for PERV γ1. In addition, novel ORF-containing PERV γ1 clones consisting of hybrid sequences were revealed. Sequence comparison from published full-length PERV γ1 clones of the PERV subfamilies A, B, and C resulted in a lack of strict correlation of the classification of pro-pol and env. The results indicated the occurrence of causative recombination events between retroviral genomes. Thus, our study on PERV γ provides new data for the evaluation and selection of pigs intended to be used in xenotransplantation.

Sign in / Sign up

Export Citation Format

Share Document