A novel simple assay system for the detection of human platelet antigen 15 (HPA‐15) alloantibodies based on three techniques: an HPA‐15 expressing cell line, a monoclonal antibody‐specific antigen‐capture method and mixed‐passive haemagglutination

Vox Sanguinis ◽  
2019 ◽  
Vol 115 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Yangsook Koh ◽  
Hiroko Nishimiya ◽  
Hiroko Inoue ◽  
Ayaka Kishigami ◽  
Yukari Tsuji ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Human Platelet ◽  
Specific Antigen ◽  
Assay System ◽  
Passive Haemagglutination ◽  
Antigen Capture ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Capture Method

Establishment of a cell line panel for the detection of antibodies against human platelet antigen 4b

2011 ◽  
Vol 93 (2) ◽  
pp. 170-175 ◽  
Author(s):  
Tomoya Hayashi ◽  
Etsuko Amakishi ◽  
Masayasu Inoue ◽  
Fumiya Hirayama
Keyword(s):  
Cell Line ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Cell Line Panel ◽  
Human Platelet Antigen ◽  
A Cell

A new bead-based human platelet antigen antibodies detection assay versus the monoclonal antibody immobilization of platelet antigens assay

Transfusion ◽  
2013 ◽  
Vol 54 (6) ◽  
pp. 1486-1492 ◽  
Author(s):  
Leendert Porcelijn ◽  
Elly Huiskes ◽  
Ilona Comijs-van Osselen ◽  
Aniska Chhatta ◽  
Vipul Rathore ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Platelet ◽  
Antibody Immobilization ◽  
Platelet Antigen ◽  
Detection Assay ◽  
Human Platelet Antigen ◽  
Antibodies Detection

Quantification of human platelet antigen-1a antibodies with the monoclonal antibody immobilization of platelet antigens procedure

Transfusion ◽  
2005 ◽  
Vol 45 (8) ◽  
pp. 1319-1323 ◽  
Author(s):  
Gérald Bertrand ◽  
Vincent Jallu ◽  
Maxime Gouet ◽  
Killie Mette Kjaer ◽  
Patrick Lambin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Platelet ◽  
Antibody Immobilization ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Characterization of a Human Platelet Antigen-1a–Specific Monoclonal Antibody Derived from a B Cell from a Woman Alloimmunized in Pregnancy

2015 ◽  
Vol 194 (12) ◽  
pp. 5751-5760 ◽  
Author(s):  
Mariana Eksteen ◽  
Heidi Tiller ◽  
Maria Averina ◽  
Gøril Heide ◽  
Mette Kjaer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
B Cell ◽  
Human Platelet ◽  
Specific Monoclonal Antibody ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
In Pregnancy

Human Platelet Antigen 3 (HPA-3): Localization of the Determinant of the Alloantibody Leka (HPA-3a) to the C-Terminus of Platelet Glycoprotein IIb Heavy Chain and Contribution of O-Linked Carbohydrates

1993 ◽  
Vol 69 (05) ◽  
pp. 485-489 ◽  
Author(s):  
Isabelle Djaffar ◽  
Didier Vilette ◽  
Dominique Pidard ◽  
Jean-Luc Wautier ◽  
Jean-Philippe Rosa
Keyword(s):  
Amino Acids ◽  
Heavy Chain ◽  
Human Platelet ◽  
Platelet Glycoprotein ◽  
Fibrinogen Receptor ◽  
C Terminus ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Determinant Structure ◽  
Polymerase Chain

SummaryThe human platelet antigen (HPA) 3 system is expressed on GPIIb, one subunit of GPIIb-IIIa, the platelet fibrinogen receptor. It was recently shown that HPA-3 was associated with an Ile843/Ser polymorphism. To investigate further HPA-3 determinant structure, we localized an HPA-3a determinant, recognized by the alloantiserum Leka, within the last 29 amino acids of GPIIbα. This region encompasses the polymorphic Ile843, which, as expected, is substituted into Ser in Leka-negative individuals, as shown by DNA sequence after polymerase chain reaction on platelet RNA. In addition, contribution of glycosylation to the determinant structure was demonstrated since the Leka antigenicity was strongly decreased after specifically removing nonterminal O-linked sugars, but not terminal sialic acids. We have thus refined the localization of an HPA-3a determinant within the last 29 amino acids, including Ile843, of GPIIb heavy chain, and shown that the Leka HPA-3a determinant is dependent, in part, upon the serine-linked carbohydrates adjacent to Ile/Ser843.

The Human Platelet Antigen HPA-la/lb (PIA1/PIA2)Polymorphism and Cerebral Ischaemia

1997 ◽  
Vol 78 (02) ◽  
pp. 964-965 ◽  
Author(s):  
Karl H Reuner ◽  
Michael Elgas ◽  
Michael Kaps ◽  
Andreas Ruf ◽  
Heinrich Patscheke
Keyword(s):  
Cerebral Ischaemia ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen
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