A new bead-based human platelet antigen antibodies detection assay versus the monoclonal antibody immobilization of platelet antigens assay

Transfusion ◽  
2013 ◽  
Vol 54 (6) ◽  
pp. 1486-1492 ◽  
Author(s):  
Leendert Porcelijn ◽  
Elly Huiskes ◽  
Ilona Comijs-van Osselen ◽  
Aniska Chhatta ◽  
Vipul Rathore ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Platelet ◽  
Antibody Immobilization ◽  
Platelet Antigen ◽  
Detection Assay ◽  
Human Platelet Antigen ◽  
Antibodies Detection

Quantification of human platelet antigen-1a antibodies with the monoclonal antibody immobilization of platelet antigens procedure

Transfusion ◽  
2005 ◽  
Vol 45 (8) ◽  
pp. 1319-1323 ◽  
Author(s):  
Gérald Bertrand ◽  
Vincent Jallu ◽  
Maxime Gouet ◽  
Killie Mette Kjaer ◽  
Patrick Lambin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Platelet ◽  
Antibody Immobilization ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Characterization of a Human Platelet Antigen-1a–Specific Monoclonal Antibody Derived from a B Cell from a Woman Alloimmunized in Pregnancy

2015 ◽  
Vol 194 (12) ◽  
pp. 5751-5760 ◽  
Author(s):  
Mariana Eksteen ◽  
Heidi Tiller ◽  
Maria Averina ◽  
Gøril Heide ◽  
Mette Kjaer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
B Cell ◽  
Human Platelet ◽  
Specific Monoclonal Antibody ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
In Pregnancy

Human Platelet Antigen 3 (HPA-3): Localization of the Determinant of the Alloantibody Leka (HPA-3a) to the C-Terminus of Platelet Glycoprotein IIb Heavy Chain and Contribution of O-Linked Carbohydrates

1993 ◽  
Vol 69 (05) ◽  
pp. 485-489 ◽  
Author(s):  
Isabelle Djaffar ◽  
Didier Vilette ◽  
Dominique Pidard ◽  
Jean-Luc Wautier ◽  
Jean-Philippe Rosa
Keyword(s):  
Amino Acids ◽  
Heavy Chain ◽  
Human Platelet ◽  
Platelet Glycoprotein ◽  
Fibrinogen Receptor ◽  
C Terminus ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Determinant Structure ◽  
Polymerase Chain

SummaryThe human platelet antigen (HPA) 3 system is expressed on GPIIb, one subunit of GPIIb-IIIa, the platelet fibrinogen receptor. It was recently shown that HPA-3 was associated with an Ile843/Ser polymorphism. To investigate further HPA-3 determinant structure, we localized an HPA-3a determinant, recognized by the alloantiserum Leka, within the last 29 amino acids of GPIIbα. This region encompasses the polymorphic Ile843, which, as expected, is substituted into Ser in Leka-negative individuals, as shown by DNA sequence after polymerase chain reaction on platelet RNA. In addition, contribution of glycosylation to the determinant structure was demonstrated since the Leka antigenicity was strongly decreased after specifically removing nonterminal O-linked sugars, but not terminal sialic acids. We have thus refined the localization of an HPA-3a determinant within the last 29 amino acids, including Ile843, of GPIIb heavy chain, and shown that the Leka HPA-3a determinant is dependent, in part, upon the serine-linked carbohydrates adjacent to Ile/Ser843.

The Human Platelet Antigen HPA-la/lb (PIA1/PIA2)Polymorphism and Cerebral Ischaemia

1997 ◽  
Vol 78 (02) ◽  
pp. 964-965 ◽  
Author(s):  
Karl H Reuner ◽  
Michael Elgas ◽  
Michael Kaps ◽  
Andreas Ruf ◽  
Heinrich Patscheke
Keyword(s):  
Cerebral Ischaemia ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sang Mee Hwang ◽  
Mi Jung Kim ◽  
Ho Eun Chang ◽  
Yun Ji Hong ◽  
Taek Soo Kim ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Human Platelet ◽  
Human Fibroblasts ◽  
Leukemia Cell ◽  
Embryonic Stem ◽  
Melting Curve ◽  
Leukemia Cell Line ◽  
Melting Curve Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.

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