Preparation of highly sensitive monoclonal antibody against α-zearalanol based on the similar antigen determinant structure to zearalanone

In this study, we report a new method to prepare highly sensitive monoclonal antibody against α-zearalanol (ZAL) based on a similar antigen determinant structure. Zearalanone (ZAN), structural analogs of ZAL, was modified by oximation to obtain ZAN-O. ZAN-O was then coupled with bovine serum albumin using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) to synthesise the artificial complete antigen ZAN-O-BSA. ZAN-O-BSA was used to immunise the BALB/c mice. The splenocytes of the immunised mice were fused with myeloma NS0 cells. During the process of cell fusion, ZAL was used as an inhibitor instead of ZAN to screen the hybridoma cell lines that can secrete monoclonal antibodies against ZAL. The sensitivity (half inhibitory concentration, IC50) of the prepared monoclonal antibody was 0.475 ng/ml, the limit of detection (LOD) was 0.050 ng/ml, the linear range of detection was 0.066-3.399 ng/ml, the affinity constant Kaff was 6.18×107 l/mol, the cross-reactivity rate with structural analogues, such as β-zearalanol, α-zearalenol, β-zearalenol, ZAN and zearalenone were 28.07, 13.16, 15.83, 60.28 and 7.95% respectively. The cross-reactivity with other mycotoxin and carrier proteins were all less than 0.05%. The prepared monoclonal antibody can be used to establish a highly sensitive immunoassay for the detection of ZAL.

Total heritage: future Eopolis for cultural urban singularity

Purpose The purpose of the article is to develop the concept of forecasting futures using the past by integrating the concept of heritage in it. Design/methodology/approach This paper demonstrates the process of development of the total heritage approach and its application. The process consists of the following steps: the review of the selected urban life visions and models of the past from prehistoric Catalhoyuk settlement up to the 20th century Japanese Metabolism to trace the examples of complex problem-solving and singularity presence that can be used for horizon scanning for the futures of urbanism; the development of total heritage approach based on the further analysis of selected examples demonstrating even unintentional presence of heritage in the construction of the futures; application of the total heritage approach for the modeling the futures of urbanism and illustrating it with the scenario of the future eopolis as the premise for cultural urban singularity. Findings This paper demonstrates the process of development of the total heritage approach and its application. The process consists of the following steps: the review of selected historic urban utopias to trace the principal scheme, how the future or ideal visions of urbanism were constructed; the development of total heritage approach based on the further analysis of Thomas More Utopia and Neolithic Catalhoyuk settlement demonstrating even unintentional presence of heritage in the construction of the futures; application of the total heritage approach for the modeling the futures of urbanism and illustrating it with the scenario of the future eopolis as the premise for cultural urban singularity. Originality/value The total heritage approach, developed in this research, presents heritage as the determinant structure or ethical imperative for sustainable future development toward cultural urban singularity.