scholarly journals Blood unit segments accurately represent the biophysical properties of red blood cells in blood bags but not hemolysis

Transfusion ◽  
2021 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Erik Lamoureux ◽  
Simon P. Duffy ◽  
Mark D. Scott ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Biophysical Properties ◽  
Blood Unit

scholarly journals Blood Unit Segments Accurately Represent the Biophysical Properties of Red Blood Cells in Blood Bags but Not Hemolysis

2021 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Erik Lamoureux ◽  
Simon P. Duffy ◽  
Mark D. Scott ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Clinical Studies ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Biophysical Properties ◽  
Distribution Width ◽  
Blood Unit ◽  
Blood Bag ◽  
Non Destructive

BACKGROUND: The biophysical properties of red blood cells (RBCs) provide potential biomarkers for the quality of donated blood. Blood unit segments provide a simple and non-destructive way to sample RBCs in clinical studies of transfusion efficacy, but it is not known whether RBCs sampled from segments accurately represent the biophysical properties of RBCs in blood bags. STUDY DESIGN AND METHODS: RBCs were sampled from blood bags and segments every two weeks during 8 weeks of storage at 4 degrees C. RBC deformability was measured by deformability-based sorting using the microfluidic ratchet device in order to derive a rigidity score. Standard hematological parameters, including mean corpuscular volume (MCV), red cell distribution width (RDW), mean cell hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and hemolysis were measured at the same time points. RESULTS: Deformability of RBCs stored in blood bags was retained over 4 weeks storage but a progressive loss of deformability was observed at weeks 6 and 8. This trend was mirrored in blood unit segments with a strong correlation to the blood bag data. Strong correlations were also observed between blood bag and segment for MCV, MCHC and MCH, but not for hemolysis. CONCLUSION: RBCs sampled from blood unit segments accurately represents the biophysical properties of RBCs in blood bags, but not hemolysis. Blood unit segments provide a simple and non-destructive sample for measuring RBC biophysical properties in clinical studies.

scholarly journals The effect of myeloperoxidase isoforms on biophysical properties of red blood cells

2019 ◽  
Vol 464 (1-2) ◽  
pp. 119-130 ◽  
Author(s):  
Ekaterina V. Shamova ◽  
Irina V. Gorudko ◽  
Daria V. Grigorieva ◽  
Alexey V. Sokolov ◽  
Anatoli U. Kokhan ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Biophysical Properties

ОСОБЕННОСТИ БИОФИЗИЧЕСКИХ СВОЙСТВ ФОРМЕННЫХ ЭЛЕМЕНТОВ КРОВИ ЛЮДЕЙ ПОЖИЛОГО ВОЗРАСТА В УСЛОВИЯХ МЕХАНИЧЕСКОГО СТРЕССА IN VITRO

2020 ◽  
Vol 65 (6) ◽  
pp. 1114-1117
Author(s):  
Е.А. Сладкова ◽  
◽  
М.Ю. Скоркина ◽  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Vascular Tone ◽  
Tissue Oxygenation ◽  
Purinergic Receptor ◽  
The Elderly ◽  
Receptor Activation ◽  
Biophysical Properties ◽  
Regulation Of Vascular Tone

This study explores the peculiarities of biophysical properties in blood cells of the elderly under mechanical stress, applied in vitro, which, according to the literature data, triggers the signaling pathways engaged by purinergic receptor activation. Along with it, we detected a decrease in the stiffness of the cell surface of red blood cells, granulocytes, lymphocytes and platelets and the surface potential became more positive. Our findings could increase knowledge about the effect of mechanical deformation on leukocytes and platelets, which are the main regulators of homeostatic processes in the microvasculature, and on red blood cells involved in the regulation of vascular tone of arterioles and tissue oxygenation in the elderly.

scholarly journals Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage

Lab on a Chip ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 448-458 ◽  
Author(s):  
Sha Huang ◽  
Han Wei Hou ◽  
Tamir Kanias ◽  
Jonas Tadeu Sertorio ◽  
Huichao Chen ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Red Cells ◽  
Prolonged Storage ◽  
Biophysical Properties ◽  
Blood Storage ◽  
Human Red Cells

In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated.

scholarly journals ЗМІНИ В КРОВІ ЩУРІВ ПІСЛЯ ІМУНІЗАЦІЇ ПРЕПАРАТОМ «ЛЕЙКОЗАВ» ПРОТИ ЛЕЙКОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 103-108
Author(s):  
A.I. Zaviriukha ◽  
O.I. Vischu ◽  
H.A. Zaviriukha
Keyword(s):  
Adverse Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Unit Volume ◽  
White Blood Cells ◽  
Statistical Error ◽  
Control Group ◽  
Blood Unit ◽  
Blood Eosinophils ◽  
Experimental Group

The article presents the results of research of hematological blood of rats under the influence of the drug «Leykozav» against bovine leukemia. Experienced animal drug «Leykozav» introduce twice at an interval of 14 days at 0.5 cm3.Before the introduction and after the introduction of studied hematologic parameters: the number of white blood cells, red blood cells and platelets and their indices, the hemoglobin content.According to the research found that the number of red blood cells, hemoglobin and hematocrit values are reduced to within statistical error, and no adverse effects on the health of laboratory animals.The introduction of the drug «Leykozav» rats did not cause any significant changes in terms of platelet indices in the blood of animals on the seventh day after the inoculation.The decrease number of segmented neutrophils and increase in the number of lymphocytes in the blood unit volume (1 at 14 and 30 days after inoculation (r < 0.05 – 0.01) increase the number of monocytes and 1,6 times at 30 days after immunization (r < 0,05) compared to before vaccination period.Influenced «Leykozav» preparation two weeks after immunization animals increases the amount of blood basophils (2 – 2,5 mkl). Compared with the control group of animals of the experimental group of blood eosinophils of rats indices are within physiological norms. 

Influence of the Purinergic Signalling System on the Properties of Human Blood Cells at Ageing

2021 ◽  
pp. 51-57
Author(s):  
Evgeniya A. Sladkova ◽  
Keyword(s):  
Older Adults ◽  
Red Blood Cells ◽  
Mechanical Stress ◽  
Blood Cells ◽  
White Blood Cells ◽  
Purinergic Signalling ◽  
Biophysical Properties ◽  
Middle Aged ◽  
Signalling System ◽  
Aged People

Adenosine triphosphate (ATP) molecules and nucleotide derivatives contribute to a wide range of cellular reactions through the activation of A2 and P2 purinergic receptors located on the cell surface. Almost any type of cells expresses such receptors, including red blood cells and white blood cells. The study of purinergic transmission of signals mediating calcium mobilization, actin polymerization, chemotaxis, release of mediators, tissue oxygenation, cell maturation, cytotoxicity, cell death and cell–cell interactions in dynamics at different stages of ontogenesis is an important issue in biology and medicine today. This paper aimed to explore the biophysical properties of blood cells during the activation of the purinergic signalling system in middle-aged people and older adults. To activate the elements of the purinergic signalling system, an in vitro model of mechanical stress was used. Blood samples were analysed using the methods of atomic force microscopy, namely, force spectroscopy and the Kelvin probe. When the purinergic signalling system is activated, an increase in ATP blood concentration is identified in the blood of both middle-aged people and older adults. In the blood of older adults, a smaller amount of ATP was recorded compared with the blood of middle-aged people, which can be due to changes in the expression of purinoreceptors on the surface of red blood cells at ageing. Erythrocytes and granulocytes in older adults are characterized by increased surface stiffness and potential upon activation of the purinergic signalling system, compared with cells of middle-aged people. In response to mechanical stress, lymphocyte membrane stiffness in older adults increases more significantly than in middle-aged people. Thus, the results indicate an important role of the ATP molecule and its derivatives in the regulation of functioning of blood cells through binding to receptors of the P2 family. Research on the changes in the biophysical properties of red blood cells and white blood cells at activation of the purinergic signalling system will allow us to identify new effects of purine compounds on intercellular interaction during ageing.

scholarly journals Hemodynamic Functionality of Stored Red Blood Cells: An Important Metric of Blood Unit Quality

2019 ◽  
Vol 108 (5) ◽  
pp. 1587-1588
Author(s):  
Saul Yedgar ◽  
Dan Arbell ◽  
Gregory Barshtein
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Unit

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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