Simultaneous blood donor screening for abnormal hemoglobin levels and glycated hemoglobin (HbA1c) by high-performance liquid chromatography

Transfusion ◽  
2015 ◽  
Vol 55 (9) ◽  
pp. 2291-2292 ◽  
Author(s):  
José Eduardo Levi ◽  
Sanny Marcele da Costa Lira ◽  
Márcia Bernardino de Carvalho Polite ◽  
Ricardo Antonio d'Almeida Pereira ◽  
Mariza Mota ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Blood Donor ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Donor Screening ◽  
Blood Donor Screening ◽  
Abnormal Hemoglobin

Application of high-performance liquid chromatography to abnormal hemoglobin studies

1981 ◽  
Vol 670 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Jean-Paul Boissel ◽  
Henri Wajcman ◽  
Hubert Fabritius ◽  
Raymond Cabannes ◽  
Dominique Labie
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Abnormal Hemoglobin

Evaluation of a Fully Automated High-Performance Liquid Chromatography Assay for Hemoglobin A1c

1999 ◽  
Vol 123 (9) ◽  
pp. 763-767 ◽  
Author(s):  
Hanh M. Khuu ◽  
C. Andrew Robinson ◽  
Kathy Goolsby ◽  
Robert W. Hardy ◽  
Robert J. Konrad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hemoglobin A1c ◽  
Blood Glucose Control ◽  
Objective Measure ◽  
Hplc Method ◽  
Diabetic Patients ◽  
Abnormal Hemoglobin ◽  
Hemoglobin Variants

Abstract Introduction.—Measurement of hemoglobin A1c (HbA1c) is used as an objective measure of long-term blood glucose control in diabetic patients. Recent improvements in automation combined with new recommendations for precision and accuracy have caused us to reevaluate our methods for measuring HbA1c. Objective.—We evaluated a newly automated high-performance liquid chromatography (HPLC) instrument for measurement of HbA1c (Tosoh A1c 2.2 Plus Glycohemoglobin Analyzer, Tosoh Medics, Foster City, Calif) and compared the results obtained by HPLC to those obtained with an immunoassay (Hitachi 911, Boehringer Mannheim Corporation, Indianapolis, Ind). Results.—The Tosoh analyzer was found to be linear in a range of 5.3% to 17% and had a throughput of 20 samples per hour. HbA1c results for 102 patient samples by the 2 techniques showed good correlation, with a slope of 0.87 and an intercept at 1.27% ± 0.15%. Both the total and within-run coefficients of variation were consistently lower for the HPLC method compared with the immunoassay method. The HPLC method produces a chromatogram that shows the different hemoglobin fractions, allowing identification of abnormal hemoglobin variants. In heterozygous individuals, HbA1c measurements are made with no interference from the hemoglobin variant. In the case of homozygous or doubly heterozygous hemoglobin variants, the Tosoh HPLC identifies the hemoglobin variants as such and correctly does not report a HbA1c value in the presence of a markedly decreased amount of hemoglobin A. Conclusions.—The Tosoh HPLC provides adequate throughput and improved precision, and the method is traceable to the Diabetes Control and Complications Trial.

scholarly journals Glycated Hemoglobin Fractions in Normal and Diabetic Cats Measured by High Performance Liquid Chromatography.

1992 ◽  
Vol 54 (4) ◽  
pp. 789-790 ◽  
Author(s):  
Shinogu HASEGAWA ◽  
Toshinori SAKA ◽  
Naoyuki TAKEMURA ◽  
Hidekazu KOYAMA ◽  
Shigekatsu MOTOYOSHI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin

scholarly journals HPLC & It’s Utilization in Disease Diagnosis

2020 ◽  
Vol 8 (2) ◽  
pp. 114-116
Author(s):  
Aadil Ansari ◽  
Ravi Gupta ◽  
Mitaksha Jhanwar ◽  
Peehu Kaushik ◽  
Divyanshu Sharma ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Illicit Drugs ◽  
Stationary Phases ◽  
Glycosylated Hemoglobin ◽  
Disease Diagnosis ◽  
Accurate Diagnosis ◽  
Abnormal Hemoglobin ◽  
Hplc Technique

High Performance Liquid Chromatography is a technique in analytical chemistry used to separate, identify and quantify each component in a mixture. The HPLC is suitable for a variety of clinical applications, including pharmaceutical development, legal application such as detecting the Presence of illicit drugs in urine & blood. High-performance liquid chromatography is a technique introduced for the accurate diagnosis of hemoglobinopathies, thalassemias & estimation of glycosylated hemoglobin. The advantage of the HPLC system is the excellent resolution, reproducibility & quantification of several normal & abnormal hemoglobin resulting in accurate diagnosis of thalassemia syndromes. HPLC technique which is used to separate the component in across the two equivalents phases i.e. stationary phases  is solid or liuqid supported on a solid  which is packed into a column while sample moves in along with mobile phase  is liuqid or gas. The purpose of this study is to evaluate the HPLC technique in diagnosis of thalassemia syndromes and also correlate it with clinicohematological profile in these cases.  

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