Effects of Sample Storage Conditions on Glycated Hemoglobin Measurement: Evaluation of Five Different High Performance Liquid Chromatography Methods

2007 ◽  
Vol 9 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Randie R. Little ◽  
Curt L. Rohlfing ◽  
Alethea L. Tennill ◽  
Shawn Connolly ◽  
Steve Hanson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Storage Conditions ◽  
Sample Storage ◽  
Measurement Evaluation ◽  
Hemoglobin Measurement

scholarly journals Glycated Hemoglobin Fractions in Normal and Diabetic Cats Measured by High Performance Liquid Chromatography.

1992 ◽  
Vol 54 (4) ◽  
pp. 789-790 ◽  
Author(s):  
Shinogu HASEGAWA ◽  
Toshinori SAKA ◽  
Naoyuki TAKEMURA ◽  
Hidekazu KOYAMA ◽  
Shigekatsu MOTOYOSHI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin

scholarly journals Glycated hemoglobin fractions in normal and diabetic dogs measured by high performance liquid chromatography.

1991 ◽  
Vol 53 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Shinogu HASEGAWA ◽  
Toshinori SAKO ◽  
Naoyuki TAKEMURA ◽  
Hidekazu KOYAMA ◽  
Shigekatsu MOTOYOSHI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Diabetic Dogs

scholarly journals Stability Determination of an Extemporaneously Compounded Ambrisentan Suspension by High Performance Liquid Chromatography Analysis

2021 ◽  
Vol 26 (3) ◽  
pp. 265-270
Author(s):  
Jesse Cramer ◽  
Mackenzie Bevry ◽  
Stephanie Handler ◽  
Kathryn Tillman ◽  
Ehab A. Abourashed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Reversed Phase ◽  
Hplc Method ◽  
Fluorescent Light

OBJECTIVE Ambrisentan, an endothelin receptor antagonist FDA-approved for the treatment of pulmonary arterial hypertension in adult patients, lacks an acceptable pediatric dosage form. The objective of this investigation was to determine the stability of an extemporaneously compounded ambrisentan suspension. METHODS Ambrisentan suspension was compounded to a concentration of 1 mg/mL using commercially available suspending agents. The suspension was then evenly split into 2 plastic amber prescription bottles. One bottle was stored at room temperature and under continuous fluorescent light while the other bottle was stored under refrigeration and protection from light. A fast and selective reversed-phase high-performance liquid chromatography (HPLC) method was developed and validated for the analysis of ambrisentan. HPLC analysis was performed on samples withdrawn from the stock bottles at predetermined time intervals, up to 90 days. RESULTS The developed HPLC method enabled the elution and detection of ambrisentan peak at 4.4 minutes. HPLC analysis revealed that all samples from both storage conditions retained >90% potency throughout the study timeframe. There were no signs of any ambrisentan breakdown products on HPLC analysis. Color and odor of the final product was also consistent throughout the 90-day storage period. CONCLUSION Ambrisentan suspension, compounded to 1 mg/mL, is stable at room temperature or under refrigeration for up to 90 days.

Glycated hemoglobin reference limits obtained by high performance liquid chromatography in adults and pregnant women

1998 ◽  
Vol 274 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Lucia S Parentoni ◽  
Eliana C de Faria ◽  
Maria José L.F Bartelega ◽  
Valéria M.S Moda ◽  
Ana Candida C Facin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pregnant Women ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Reference Limits
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