Multi-gene silencing in Arabidopsis: a collection of artificial microRNAs targeting groups of paralogs encoding transcription factors

2014 ◽  
Vol 80 (1) ◽  
pp. 149-160 ◽  
Author(s):  
Sara Jover-Gil ◽  
Javier Paz-Ares ◽  
José Luis Micol ◽  
María Rosa Ponce
Keyword(s):  
Transcription Factors ◽  
Gene Silencing

scholarly journals Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway

2013 ◽  
Vol 40 (10) ◽  
pp. 1029 ◽  
Author(s):  
Aguida M. A. P. Morales ◽  
Jamie A. O'Rourke ◽  
Martijn van de Mortel ◽  
Katherine T. Scheider ◽  
Timothy J. Bancroft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Gene Silencing ◽  
Differentially Expressed ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
R Gene ◽  
Virus Induced Gene Silencing ◽  
Asian Soybean Rust ◽  
Defence Responses

Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes repressed at 12 hai were not differentially expressed from 24 to 144 hai, but were repressed 216 hai and later. To differentiate between basal and resistance-gene (R-gene) mediated defence responses, we compared gene expression in Rpp4-silenced and empty vector-treated PI459025B plants 14 days after infection (dai) with P. pachyrhizi. This identified genes, including transcription factors, whose differential expression is dependent upon Rpp4. To identify differentially expressed genes conserved across multiple P. pachyrhizi resistance pathways, Rpp4 expression datasets were compared with microarray data previously generated for Rpp2 and Rpp3-mediated defence responses. Fourteen transcription factors common to all resistant and susceptible responses were identified, as well as fourteen transcription factors unique to R-gene-mediated resistance responses. These genes are targets for future P. pachyrhizi resistance research.

scholarly journals Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids

2013 ◽  
Vol 64 (12) ◽  
pp. 3869-3884 ◽  
Author(s):  
Ming-Hsien Hsieh ◽  
Zhao-Jun Pan ◽  
Pei-Han Lai ◽  
Hsiang-Chia Lu ◽  
Hsin-Hung Yeh ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Silencing ◽  
Growth And Development ◽  
Virus Induced Gene Silencing

scholarly journals Novel vector systems to accelerate functional analysis of transcription factors using chimeric repressor gene-silencing technology (CRES-T)

2011 ◽  
Vol 28 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Yoshimi Oshima ◽  
Nobutaka Mitsuda ◽  
Masaru Nakata ◽  
Tsuyoshi Nakagawa ◽  
Shingo Nagaya ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcription Factors ◽  
Gene Silencing ◽  
Repressor Gene ◽  
Vector Systems

scholarly journals Environment‐responsive transcription factors bind subtelomeric elements and regulate gene silencing

2011 ◽  
Vol 7 (1) ◽  
pp. 455 ◽  
Author(s):  
Jennifer J Smith ◽  
Leslie R Miller ◽  
Richard Kreisberg ◽  
Laura Vazquez ◽  
Yakun Wan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Silencing ◽  
Regulate Gene

scholarly journals The H3.3K27M oncohistone antagonizes reprogramming in Drosophila

2020 ◽  
Author(s):  
Kami Ahmad ◽  
Steven Henikoff
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Gene Silencing ◽  
Master Regulator ◽  
Histone H3k27 ◽  
Chromatin Profiling ◽  
Polycomb Repressive Complex 1 ◽  
Development Proceeds ◽  
Simple Combination

AbstractDevelopment proceeds by the activation of genes by transcription factors and the inactivation of others by chromatin-mediated gene silencing. In some cases development can be reversed or redirected by mis-expression of master regulator transcription factors. This must involve the activation of previously silenced genes, and such developmental aberrations are thought to underlie a variety of cancers. Here, we express the wing-specific Vestigial master regulator to reprogram the developing eye, and test the role of silencing in reprogramming using an H3.3K27M oncohistone mutation that dominantly inhibits histone H3K27 trimethylation. We find that expression of the oncohistone blocks eye-to-wing reprogramming. CUT&Tag chromatin profiling of mutant tissues shows that H3K27me3 domains are globally reduced with oncohistone expression, suggesting that previous developmental programs must be silenced for effective transformation. Strikingly, mis-expressed Vg and H3.3K27M synergize to stimulate overgrowth of eye tissue, a phenotype that resembles that of mutations in Polycomb Repressive Complex 1 components. Our results imply that growth dysregulation can result from the simple combination of crippled silencing and transcription factor mis-expression, an effect that may explain the origins of oncohistone-bearing cancers.

scholarly journals A High-Throughput Virus-Induced Gene-Silencing Vector for Screening Transcription Factors in Virus-Induced Plant Defense Response in Orchid

2012 ◽  
Vol 25 (6) ◽  
pp. 738-746 ◽  
Author(s):  
Hsiang-Chia Lu ◽  
Ming-Hsien Hsieh ◽  
Cheng-En Chen ◽  
Hong-Hwa Chen ◽  
Hsiang-Iu Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Silencing ◽  
Plant Defense ◽  
High Throughput ◽  
Viral Vector ◽  
Defense Responses ◽  
Dna Fragments ◽  
Virus Induced Gene Silencing ◽  
High Throughput Analysis ◽  
Related Plant

The large number of species and worldwide spread of species of Orchidaceae indicates their successful adaptation to environmental stresses. Thus, orchids provide rich resources to study how plants have evolved to cope with stresses. This report describes our improvement of our previously reported orchid virus-induced gene silencing vector, pCymMV-pro60, with a modified Gateway cloning system which requires only one recombination and can be inoculated by agroinfiltration. We cloned 1,700 DNA fragments, including 187 predicted transcription factors derived from an established expression sequence tag library of orchid, into pCymMV-Gateway. Phalaenopsis aphrodite was inoculated with these vectors that contained DNA fragments of the 187 predicted transcription factors. The viral vector initially triggered the expression of the salicylic acid (SA)-related plant defense responses and later induced silencing of the endogenous target transcription factor genes. By monitoring the expression of the SA-related plant defense marker PhaPR1 (homolog of PR1), we identified a gene, PhaTF15, involved in the expression of PhaPR1. Knockdown of PhaTF15 by virus-induced gene silencing and by transient delivery of double-stranded RNA (dsRNA) reduced expression of the orchid homolog of the conserved positive defense regulator NPR1, PhaNPR1. Cymbidium mosaic virus also accumulated to high levels with knockdown of PhaTF15 by transient delivery of dsRNA. We demonstrated efficient cloning and screening strategies for high-throughput analysis of orchid and identify a gene, PhaTF15, involved in regulation of SA-related plant defense.

SUMO-modified transcription factors repress transcription by provoking local heterochromatic gene silencing

2008 ◽  
Vol 2008 (Spring) ◽  
Author(s):  
Bastian Stielow ◽  
Alexandra Sapetschnig ◽  
Christina Wink ◽  
Guntram Suske
Keyword(s):  
Transcription Factors ◽  
Gene Silencing

scholarly journals PPARα, δ and FOXO1 Gene Silencing Overturns Palmitate-Induced Inhibition of Pyruvate Oxidation Differentially in C2C12 Myotubes

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1098
Author(s):  
Hung-Che Chien ◽  
Despina Constantin ◽  
Paul L. Greenhaff ◽  
Dumitru Constantin-Teodosiu
Keyword(s):  
Transcription Factors ◽  
Protein Expression ◽  
Gene Silencing ◽  
Glucose Uptake ◽  
Glucose Oxidation ◽  
Molecular Mechanisms ◽  
C2c12 Myotubes ◽  
Atp Production ◽  
Pyruvate Oxidation ◽  
Metabolic Inflexibility

The molecular mechanisms by which free fatty acids (FFA) inhibit muscle glucose oxidation is still elusive. We recently showed that C2C12 myotubes treated with palmitate (PAL) presented with greater protein expression levels of PDK4 and transcription factors PPARα and PPARδ and lower p-FOXO/t-FOXO protein ratios when compared to control. This was complemented with the hallmarks of metabolic inflexibility (MI), i.e., reduced rates of glucose uptake, PDC activity and maximal pyruvate-derived ATP production rates (MAPR). However, the relative contribution of these transcription factors to the increase in PDK4 and reduced glucose oxidation could not be established. Therefore, by using a similar myotube model, a series of individual siRNA gene silencing experiments, validated at transcriptional and translation levels, were performed in conjunction with measurements of glucose uptake, PDC activity, MAPR and concentrations of metabolites reflecting PDC flux (lactate and acetylcarnitine). Gene silencing of PPARα, δ and FOXO1 individually reduced PAL-mediated inhibition of PDC activity and increased glucose uptake, albeit by different mechanisms as only PPARδ and FOXO1 silencing markedly reduced PDK4 protein content. Additionally, PPARα and FOXO1 silencing, but not PPARδ, increased MAPR with PAL. PPARδ silencing also decreased FOXO1 protein. Since FOXO1 silencing did not alter PPARδ protein, this suggests that FOXO1 might be a PPARδ downstream target. In summary, this study suggests that the molecular mechanisms by which PAL reduces PDC-mediated glucose-derived pyruvate oxidation in muscle occur primarily through increased PPARδ and FOXO1 mediated increases in PDK4 protein expression and secondarily through PPARα mediated allosteric inhibition of PDC flux. Furthermore, since PPARδ seems to control FOXO1 expression, this may reflect an important role for PPARδ in preventing glucose oxidation under conditions of increased lipid availability.

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