Characterisation of clinicalStaphylococcus aureusisolates harbouringmecAor Panton-Valentine leukocidin genes from four tertiary care hospitals in Indonesia

2016 ◽  
Vol 21 (5) ◽  
pp. 610-618 ◽  
Author(s):  
Dewi Santosaningsih ◽  
Sanarto Santoso ◽  
Nyoman S. Budayanti ◽  
Ketut Suata ◽  
Endang S. Lestari ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Tertiary Care Hospitals ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

scholarly journals High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-AcquiredStaphylococcus aureusIsolated from Tertiary Care Hospitals in Nepal

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Bidya Shrestha ◽  
Winny Singh ◽  
V. Samuel Raj ◽  
Bharat Mani Pokhrel ◽  
Tribhuban Mohan Mohapatra
Keyword(s):  
Tertiary Care ◽  
Hospital Setting ◽  
Restriction Pattern ◽  
High Prevalence ◽  
Invasive Diseases ◽  
Life Threatening ◽  
Tertiary Care Hospitals ◽  
Gene Restriction ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Methicillin-resistantStaphylococcus aureus(MRSA) carrying the important virulence determinant, Panton-Valentine leukocidin (PVL), is an emerging infectious pathogen associated with skin and soft tissue infections as well as life-threatening invasive diseases. In carrying out the first PVL prevalence study in Nepal, we screened 73 nosocomial isolates ofS. aureusfrom 2 tertiary care Nepali hospitals and obtained an overall PVL-positivity rate of 35.6% among the hospital isolates: 26.1% of MRSA and 51.9% of methicillin sensitiveS. aureus(MSSA) isolates were found to be positive for the PVL genes. PVL prevalence was not associated with a specific (i) infection site, (ii) age group, or (iii) hospital of origin. It was found to be positively associated with heterogeneous MRSA (73.3%) compared to homogeneous MRSA (3.2%) and MSSA (51.9%); negatively associated with multiresistant MRSA (22%) compared to nonmultiresistant MRSA (60%) and MSSA (51.9%); and positively associated with macrolide-streptogramin B resistance (93.8%) compared to macrolide-lincosamide-streptogramin B resistance (0%) and no-resistance (45.8%) types. Macrolide-streptogramin B resistance was confirmed by the presence ofmsr(A) gene. Restriction pattern analyses provided evidence to support the circulation of a limited number of clones of PVL-positive MRSA, arguing for the adaptability of these isolates to a hospital setting.

scholarly journals High rates of community-acquired, Panton-Valentine leukocidin (PVL)- positive methicillin-resistant S. aureus (MRSA) infections in adult outpatients in Greece

2009 ◽  
Vol 14 (2) ◽  
Author(s):  
S Vourli ◽  
H Vagiakou ◽  
G Ganteris ◽  
M Orfanidou ◽  
M Polemis ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Sccmec Type ◽  
Tertiary Care ◽  
Empiric Therapy ◽  
Care Hospital ◽  
Spa Typing ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Staphylococcus aureus was isolated in 88 (30. 8%) of 286 adult patients suffering from various skin and soft-tissue infections examined in the outpatient department of a 650 bed tertiary-care hospital of Athens, Greece between January 2006 and December 2007. Twenty-seven (30.7%) of the S. aureus infections were caused by methicillin-resistant S. aureus (MRSA). All MRSA isolates were also resistant to tetracycline, fucidic acid and kanamycin, but were sensitive to gentamicin and tobramycin, as well as to to cotrimoxazole, chloramphenicol, quinolones, clindamycin and erythromycin. All isolates belonged to staphylococcal cassette chromosome mec elements (SCCmec) type IV, and were found to carry the lukF-PV and lukS genes coding for Panton-Valentine leukocidin (PVL). Pulsed-field gel electrophoresis (PFGE) and spa-typing revealed high genetic similarity among all MRSA isolates and with the PFGE pattern of the well-described ST80 clone that seems to be spreading through Europe. The high prevalence of MRSA among S. aureus infections in the community signify that empiric therapy in Greece, when clinically indicated, should exclude β-lactam antibiotics. Moreover, the establishment of an active screening for PVL-positive community-acquired (CA)-MRSA carriage and the adoption of a search and destroy strategy for CA-MRSA in all patients admitted with purulent skin and soft-tissue is of high priority in Greece as well as in all European countries which face high rates of CA-MRSA infection.

scholarly journals Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city

2018 ◽  
Vol 12 (09) ◽  
pp. 720-725
Author(s):  
Muhammad Shaheen Iqbal ◽  
Yasar Saleem ◽  
Farheen Ansari ◽  
Muhammad Usman Qamar ◽  
Sania Mazhar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Meca Gene ◽  
Toxin Gene ◽  
Mannitol Salt Agar ◽  
Burn Wounds ◽  
High Level ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Introduction: Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates. Methodology: This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients’ departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR. Results: We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively. Conclusions: A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.

scholarly journals High Incidence of Panton-Valentine Leukocidin-Producing Staphylococcus aureus in a Tertiary Care Public Hospital in Singapore

2005 ◽  
Vol 40 (3) ◽  
pp. 486-489 ◽  
Author(s):  
H. Li-Yang ◽  
K. Tse-Hsien ◽  
K. Asok ◽  
L. Jenny ◽  
P. C. Maciej ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Public Hospital ◽  
Tertiary Care ◽  
High Incidence ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

CIScoSI Study [Children Immunization Status and cases of Systemic Illness]

2018 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Infectious Diseases ◽  
East Asia ◽  
Pediatric Patients ◽  
Severe Disease ◽  
Tertiary Care ◽  
Immunization Coverage ◽  
South East Asia ◽  
Immunization Status ◽  
Tertiary Care Hospitals ◽  
Total Coverage

To determine the immunization status of pediatric patients under age of 5 years visiting pediatric department of tertiary care hospitals in South East Asia. The aim of this study was to appreciate the awareness and implementation of vaccination in pediatric patients who came into pediatric outpatient Department with presenting complain other than routine vaccination. we can also know the count of patients who do not complete their vaccination after birth. we can differentiate between vaccinated and unvaccinated patients and incidence of severe disease in both groups. Immunization is a protective process which makes a person resistant to the harmful diseases prevailing in the community, typically by vaccine administration either orally or intravenously. It is proven for controlling and eliminating many threatening diseases from the community. WHO report that licensed vaccines are available for the prevention of many infectious diseases. After the implementation of effective immunization the rate of many infectious diseases have declined in many countries of the world. South-East Asia is far behind in the immunization coverage. An estimated total coverage is 56%-88% for a fully immunized child, which is variable between countries. Also the coverage is highest for BCG and lowest for Polio.

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