SpectraCam® : A new polarized hyperspectral imaging system for repeatable and reproducible in vivo skin quantification of melanin, total hemoglobin, and oxygen saturation

2017 ◽  
Vol 24 (1) ◽  
pp. 99-107 ◽  
Author(s):  
A. Nkengne ◽  
J. Robic ◽  
P. Seroul ◽  
S. Gueheunneux ◽  
M. Jomier ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Hyperspectral Imaging ◽  
Imaging System ◽  
Total Hemoglobin

scholarly journals A polarized hyperspectral imaging system for in vivo detection: Multiple applications in sunflower leaf analysis

2019 ◽  
Vol 158 ◽  
pp. 258-270 ◽  
Author(s):  
Jun-Li Xu ◽  
Alexia Gobrecht ◽  
Daphné Héran ◽  
Nathalie Gorretta ◽  
Marie Coque ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Imaging System ◽  
Leaf Analysis ◽  
In Vivo Detection

A hyperspectral imaging system for in vivo optical diagnostics

2004 ◽  
Vol 23 (5) ◽  
pp. 40-49 ◽  
Author(s):  
Tuan Vo-Dihn ◽  
D.L. Stokes ◽  
M.B. Wabuyele ◽  
M.E. Martin ◽  
Joon Myong Song ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Imaging System ◽  
Optical Diagnostics

Polarized hyperspectral imaging system for in vivo detection of vulvar lichen sclerosis

2016 ◽  
Author(s):  
Yingjie Qu ◽  
Wenqi Ren ◽  
Songde Liu ◽  
Peng Liu ◽  
Lan Xie ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Imaging System ◽  
Lichen Sclerosis ◽  
In Vivo Detection

scholarly journals In Vivo Assessment of Hypoxia Levels in Pancreatic Tumors Using a Dual-Modality Ultrasound/Photoacoustic Imaging System

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 668
Author(s):  
Yuhling Wang ◽  
De-Fu Jhang ◽  
Chia-Hua Tsai ◽  
Nai-Jung Chiang ◽  
Chia-Hui Tsao ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Oxygen Saturation ◽  
Pancreatic Tumor ◽  
Tissue Oxygenation ◽  
Imaging System ◽  
Tissue Oxygen Saturation ◽  
Functional Information ◽  
Tissue Oxygen ◽  
Dual Modality

Noninvasive anatomical and functional imaging has become an essential tool to evaluate tissue oxygen saturation dynamics in preclinical or clinical studies of hypoxia. Our dual-wavelength technique for photoacoustic (PA) imaging based on the differential absorbance spectrum of oxyhemoglobin (oxy-Hb) and deoxyhemoglobin (deoxy-Hb) can quantify tissue oxygen saturation using the intrinsic contrast property. PA imaging of tissue oxygen saturation can be used to monitor tumor-related hypoxia, which is a particularly relevant functional parameter of the tumor microenvironment that has a strong influence on tumor aggressiveness. The simultaneous acquisition of anatomical and functional information using dual-modality ultrasound (US) and PA imaging technology enhances the preclinical applicability of the method. Here, the developed dual-modality US/PA system was used to measure relative tissue oxygenation using the dual-wavelength technique. Tissue oxygen saturation was quantified in a pancreatic tumor mouse model. The differences in tissue oxygenation were detected by comparing pancreatic samples from normal and tumor-bearing mice at various time points after implantation. The use of an in vivo pancreatic tumor model revealed changes in hypoxia at various stages of tumor growth. The US/PA imaging data positively correlated with the results of immunohistochemical staining for hypoxia. Thus, our dual-modality US/PA imaging system can be used to reliably assess and monitor hypoxia in pancreatic tumor mouse models. These findings enable the use of a combination of US and PA imaging to acquire anatomical and functional information on tumor growth and to evaluate treatment responses in longitudinal preclinical studies.

scholarly journals Functional Imaging of the Ocular Fundus Using an 8-Band Retinal Multispectral Imaging System

Instruments ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 12
Author(s):  
Emanuel R. de Carvalho ◽  
Richelle J. M. Hoveling ◽  
Cornelis J. F. van Noorden ◽  
Reinier O. Schlingemann ◽  
Maurice C. G. Aalders
Keyword(s):  
Oxygen Saturation ◽  
Functional Imaging ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Imaging Techniques ◽  
Incident Light ◽  
Contrast Imaging ◽  
Blood Oxygen Saturation ◽  
Full Scan

Application of functional imaging in ophthalmology requires efficient imaging techniques that can detect and quantify chromophores to visualise processes in vivo. The aim of the present study was to develop and evaluate a fast and affordable imaging system. We describe an eight-band retinal multispectral imaging (MSI) system and compare it with a hyperspectral imaging (HSI) device. Determination of blood oxygen saturation was studied as proof of principle. Reflectance of incident light is measured as 1/absorbance at different wavelengths between 440 nm and 580 nm. Both devices have incorporated optical bandpass filters in a mydriatic fundus camera. The MSI system scans the retina at eight pre-defined wavelengths specific for the spectrum of haemoglobin. The HSI system acquires a full scan from 480 to 720 nm in 5 nm steps. A simple assessment of the ratio between the absorbance peaks of oxygenated haemoglobin (HbO2) and reduced haemoglobin (HbR) was not suitable for generating validated oxygenation maps of the retina. However, a correction algorithm that compares the measured reflectance with reflectance spectra of fully oxygenated and fully deoxygenated blood allowed our MSI setup to estimate relative oxygen saturation at higher levels, but underestimated relative oxygen saturation at lower levels. The MSI device generated better quality images than the HSI device. It allows customisation with filter sets optimised for other chromophores of interest, and augmented with extrinsic contrast imaging agents, it has the potential for a wider range of ophthalmic molecular imaging applications.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

scholarly journals Nanotechnology: from In Vivo Imaging System to Controlled Drug Delivery

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Maria Mir ◽  
Saba Ishtiaq ◽  
Samreen Rabia ◽  
Maryam Khatoon ◽  
Ahmad Zeb ◽  
...  
Keyword(s):  
Drug Delivery ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Controlled Drug Delivery ◽  
In Vivo Imaging System
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