A small RNA targets pokeweed antiviral protein transcript

2015 ◽  
Vol 156 (3) ◽  
pp. 241-251 ◽  
Author(s):  
Alexander Klenov ◽  
Kira C. M. Neller ◽  
Lydia A. Burns ◽  
Gabriela Krivdova ◽  
Katalin A. Hudak
Keyword(s):  
Small Rna ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Rna Targets

Development of a double sandwich ELISA able to discriminate between free PAP (pokeweed antiviral protein) and complexed PAP in leaf extracts

Plant Science ◽  
1999 ◽  
Vol 140 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jean-Luc Schlick ◽  
Bénédicte Desvoyes ◽  
Mehdi Hakil ◽  
Pascale Adami ◽  
Philippe Dulieu
Keyword(s):  
Sandwich Elisa ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Leaf Extracts

The 2·5 Å Structure of Pokeweed Antiviral Protein

1993 ◽  
Vol 233 (4) ◽  
pp. 705-715 ◽  
Author(s):  
Arthur F. Monzingo ◽  
Edward J. Collins ◽  
Stephen R. Ernst ◽  
James D. Irvin ◽  
Jon D. Robertus
Keyword(s):  
Pokeweed Antiviral Protein ◽  
Antiviral Protein

scholarly journals CleaveLand: a pipeline for using degradome data to find cleaved small RNA targets

2008 ◽  
Vol 25 (1) ◽  
pp. 130-131 ◽  
Author(s):  
C. Addo-Quaye ◽  
W. Miller ◽  
M. J. Axtell
Keyword(s):  
Small Rna ◽  
Rna Targets

scholarly journals Expression of a Truncated Form of Ribosomal Protein L3 Confers Resistance to Pokeweed Antiviral Protein and the Fusarium Mycotoxin Deoxynivalenol

2005 ◽  
Vol 18 (8) ◽  
pp. 762-770 ◽  
Author(s):  
Rong Di ◽  
Nilgun E. Tumer
Keyword(s):  
Ribosomal Protein ◽  
Fusarium Species ◽  
Common Mechanism ◽  
Pokeweed Antiviral Protein ◽  
Wild Type ◽  
Antiviral Protein ◽  
Trichothecene Mycotoxin ◽  
Truncated Form ◽  
Fusarium Mycotoxin ◽  
Ribosomal Protein L3

The contamination of important agricultural products such as wheat, barley, or maize with the trichothecene mycotoxin deoxynivalenol (DON) due to infection with Fusarium species is a worldwide problem. Trichothecenes inhibit protein synthesis by targeting ribosomal protein L3. Pokeweed antiviral protein (PAP), a ribosome-inactivating protein binds to L3 to depurinate the α–sarcin/loop of the large rRNA. Plants transformed with the wild-type PAP show lesions and express very low levels of PAP because PAP autoregulates its expression by destabilizing its own mRNA. We show here that transgenic tobacco plants expressing both the wild-type PAP and a truncated form of yeast L3 (L3δ) are phenotypically normal. PAP mRNA and protein levels are very high in these plants, indicating that L3δ suppresses the autoregulation of PAP mRNA expression. Ribosomes are not depurinated in the transgenic plants expressing PAP and L3δ, even though PAP is associated with ribosomes. The expression of the endogenous tobacco ribosomal protein L3 is up-regulated in these plants and they are resistant to the Fusarium mycotoxin DON. These results demonstrate that expression of an N-terminal fragment of yeast L3 leads to trans-dominant resistance to PAP and the trichothecene mycotoxin DON, providing evidence that both toxins target L3 by a common mechanism.

Synergistic anti-Newcastle disease virus activity of pokeweed antiviral protein, ribavirin, and guanidine

1998 ◽  
Vol 44 (7) ◽  
pp. 702-705 ◽  
Author(s):  
Eric Weaver ◽  
Gary M Aron
Keyword(s):  
Newcastle Disease Virus ◽  
Key Words ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Virus Activity

Pokeweed antiviral protein (PAP), in combination with either guanidine or ribavirin, exhibited both synergy and antagonism toward the replication of Newcastle disease virus. Low, medium, and high effective concentrations of both PAP and guanidine were synergistic when used in combination. High effective concentrations of ribavirin in combination with PAP were antagonistic, whereas low and medium effective concentrations were synergistic. Key words: pokeweed antiviral protein, Newcastle disease virus, ribavirin, guanidine, synergy.

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