Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana

2012 ◽  
Vol 11 (3) ◽  
pp. 315-324 ◽  
Author(s):  
Hui-Ting Chan ◽  
Min-Yuan Chia ◽  
Victor Fei Pang ◽  
Chian-Ren Jeng ◽  
Yi-Yin Do ◽  
...  
Keyword(s):  
Virus Antigen ◽  
Respiratory Syndrome Virus ◽  
Transgenic Banana ◽  
Oral Immunogenicity

scholarly journals Immunohistochemical Detection of Porcine Reproductive and Respiratory Syndrome Virus Antigen in Formalin-Fixed, Paraffin-Embedded Tissues with Correlation to Clinicopathologic Data

2017 ◽  
Vol 3 (6) ◽  
pp. 252
Author(s):  
Lavina Gracia G. Manzano
Keyword(s):  
Animal Health ◽  
Virus Antigen ◽  
The Philippines ◽  
Respiratory Syndrome Virus ◽  
Tissue Samples ◽  
Follicular Hyperplasia ◽  
Prrs Virus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Immunohistochemistry (IHC) was used in this study to detect the presence of porcine reproductive and respiratory virus (PRRSV) antigen using monoclonal antibody (Mab SDOW17) in formalin-fixed, paraffin embedded tissues of lung, lymph node, heart, spleen, and kidney of pre- weaning to less than 90 day old pigs. Out of the 25 tissue samples examined from swine cases of the Philippine Animal Health Center (PAHC), Bureau of Animal Industry and College of Veterinary Medicine (CVM) Histopathology Laboratory, University of the Philippines-Los Baños (UPLB), 14 or 56% (14/25) were IHC positive for PRRSV antigen.  Among the selected tissue samples, the PRRS virus was detected the most in the lymph nodes (64%) and lungs (40%), respectively, compared to other organs such as spleen (33%), kidney (28%), and the heart (17%). Only 43% (6/14) of cases coincided with the pathogenesis and clinicopathologic lesions of PRRS which are proliferative interstitial pneumonia and lymphoid follicular hyperplasia and necrosis. PRRS positive cases in IHC were consistently found to have co-infections with viral and bacterial diseases. Since PRRSV has tropism for macrophages and destruction of these cells leads to immunosuppression, affected animals are vulnerable to secondary infections. Keywords: formalin-fixed;  immunohistochemistry;  paraffin embedded tissue;  piglets;  Porcine Reproductive and Respiratory Syndrome; PRRS virus antigen; Mab SDOW17 

scholarly journals Development of a Streptavidin-Biotin Immunoperoxidase Procedure for the Detection of Porcine Reproductive and Respiratory Syndrome Virus Antigen in Porcine Lung

1994 ◽  
Vol 6 (2) ◽  
pp. 254-257 ◽  
Author(s):  
Patrick G. Halbur ◽  
John J. Andrews ◽  
Elise L. Huffman ◽  
Prem S. Paul ◽  
Xiang-Jin Meng ◽  
...  
Keyword(s):  
Virus Antigen ◽  
Respiratory Syndrome Virus

scholarly journals Comparison of the Antigen Distribution of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus

1996 ◽  
Vol 33 (2) ◽  
pp. 159-170 ◽  
Author(s):  
P. G. Halbur ◽  
P. S. Paul ◽  
M. L. Frey ◽  
J. Landgraf ◽  
K. Eernisse ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Nodes ◽  
Cell Types ◽  
Virus Antigen ◽  
Respiratory Syndrome Virus ◽  
Control Groups ◽  
Lelystad Virus ◽  
And Control ◽  
Virus Isolates ◽  
Formalin Fixed

One hundred 4-week-old cesarean-derived colostrum-deprived pigs were inoculated with one of two different US porcine reproductive and respiratory syndrome virus (PRRSV) isolates (VR2385, VR2431) or the European Lelystad virus to detect and compare the location and amount of virus antigen. Interstitial pneumonia, myocarditis, lymphadenopathy, and encephalitis were consistently seen in all three groups; however, disease and lesions were more severe in the VR2385 group. Immunohistochemical evaluation of formalin-fixed tissues revealed virus antigen in alveolar macrophages in lungs of 22/25, 14/25, 14/25, and 0/25 of the VR2385, VR2431, Lelystad, and control pigs, respectively. Follicular macrophages and dendritic cells in the lymph nodes of 14/25, 10/25, 10/25, and 0/25 pigs from the VR2385, VR2431, Lelystad, and control groups, respectively, stained positive for virus antigen. Similar cells in the tonsils from 25/25, 21/25, 23/25, and 0/25 pigs from the VR2385, VR2431, Lelystad, and control groups, respectively, stained positive for virus antigen. Other tissues and cells in which virus antigen was detected included macrophages and endothelial cells in the heart, macrophages, and interdigitating cells in the thymus, macrophages and dendritic cells in the spleen and Peyer's patches, and macrophages in hepatic sinusoids, renal medullary interstitium, and adrenal gland. PRRSV persisted in macrophages in the lung, tonsil, lymph node, and spleen for at least 28 days. Significantly more PRRSV antigen was detected in the lung ( P < 0.01), lymph nodes ( P ≤ 0.05), and tonsils ( P < 0.05) of the VR2385 pigs than was detected in the same tissues of the VR2431 and Lelystad pigs. The cell types in which PRRSV antigen was detected and the distribution of PRRSV antigen-positive cells within particular tissues and organs were generally similar for the different virus inoculation groups despite differences in virulence of the isolates.

scholarly journals Cultural properties of swine reproductive and respiratory syndrome virus isolate

2020 ◽  
Vol 195 (4) ◽  
pp. 71-77
Author(s):  
E. Markova ◽  
L. Lyulkova ◽  
R. Melnik ◽  
V. Popova
Keyword(s):  
Cell Culture ◽  
Virus Isolate ◽  
Polyclonal Antibodies ◽  
Vero Cells ◽  
Virus Genome ◽  
Virus Antigen ◽  
Respiratory Syndrome Virus ◽  
Cell Infection ◽  
High Biological Activity ◽  
Cultural Properties

Abstract. This article summarizes the results of a study of the cultural properties of the swine reproductive and respiratory syndrome virus isolate in transferable cultures of MA-104, PK-15, MARC-145 and Vero cells. The purpose of our research was to determine the sensitivity of cell culture lines to the swine reproductive and respiratory syndrome virus isolate, which is necessary for obtaining a highly active antigen as the main component of diagnostic and vaccine biologics. An isolate of the virus was isolated from a Piglet in the LPH of the Moscow region of the Kolomenskoye district. The isolated isolate of an infectious disease of pigs by the method of molecular biological analysis is characterized in the established order for this pathogen. Novelty. The possibility of reproduction in MARC-145 cell culture has been demonstrated. Results. It was shown that during reproduction of the virus isolate in cell culture for 96 ± 6 hours at a dose of 0.1 TCD50 / cell infection, an antigen with high biological activity was obtained. Infectious activity of the virus on the MARC-145 culture averaged 5.51 ± 0.45 lg TCD50/cm3 after the first three passages (after adaptation). Real-time PCR confirmed the presence of the virus genome in the test samples. Detection of the virus antigen in an infected cell culture was determined by the manifestation of a specific glow in the indirect immunofluorescence (RNIF) reaction in the cell culture monolayer fixed with acetone. It was found that FITZ-conjugate of rabbit polyclonal antibodies to pig immunoglobulins detected antigen-containing cells due to intense illumination.

In vivo targeting of porcine reproductive and respiratory syndrome virus antigen through porcine DC-SIGN to dendritic cells elicits antigen-specific CD4T cell immunity in pigs

Vaccine ◽  
2014 ◽  
Vol 32 (50) ◽  
pp. 6768-6775 ◽  
Author(s):  
Sakthivel Subramaniam ◽  
Pablo Piñeyro ◽  
Debin Tian ◽  
Christopher Overend ◽  
Danielle M. Yugo ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Virus Antigen ◽  
Respiratory Syndrome Virus ◽  
Cell Immunity ◽  
Cd4t Cell
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