Parallel enlargement of Marinesco bodies and nuclei and progressive deposition of p62 in pigmented neurons of the substantia nigra

2020 ◽  
Vol 40 (4) ◽  
pp. 328-335
Author(s):  
Ryota Amano ◽  
Shuta Toru ◽  
Michio Yamane ◽  
Masanobu Kitagawa ◽  
Katsuiku Hirokawa ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Marinesco Bodies

Intranuclear Aggregation of Nonexpanded Ataxin-3 in Marinesco Bodies of the Nonhuman Primate Substantia Nigra

2002 ◽  
Vol 176 (1) ◽  
pp. 117-121 ◽  
Author(s):  
Mattias Kettner ◽  
Dirk Willwohl ◽  
Gene B. Hubbard ◽  
U. Rüb ◽  
Edward J. Dick ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Nonhuman Primate ◽  
Marinesco Bodies

Distribution of Marinesco Bodies in Human Substantia Nigra Neurons

2016 ◽  
Vol 46 (7) ◽  
pp. 839-842
Author(s):  
I. P. Grigor’ev ◽  
V. V. Gusel’nikova ◽  
E. G. Sukhorukova ◽  
D. E. Korzhevskii
Keyword(s):  
Substantia Nigra ◽  
Marinesco Bodies ◽  
Human Substantia Nigra

scholarly journals The Spatial Organization of the Intranuclear Structures of Human Brain Dopaminergic Neurons

Acta Naturae ◽  
2017 ◽  
Vol 9 (3) ◽  
pp. 81-88 ◽  
Author(s):  
D. E. Korzhevskii ◽  
V. V. Gusel’nikova ◽  
O. V. Kirik ◽  
E. G. Sukhorukova ◽  
I. P. Grigorev
Keyword(s):  
Substantia Nigra ◽  
Dopaminergic Neurons ◽  
Spatial Organization ◽  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Selective Vulnerability ◽  
Confocal Laser ◽  
Double Labeling ◽  
Dimensional Reconstruction ◽  
Marinesco Bodies

We studied the intranuclear localization of protein nucleophosmin (B23) and ubiquitin in the dopaminergic neurons of human substantia nigra (n = 6, age of 25-87 years) using immunohistochemistry and confocal laser microscopy. Intranuclear ubiquitin-immunopositive bodies that morphologically correspond to Marinesco bodies were found to be present in substantia nigra dopaminergic (tyrosine hydroxylase-immunopositive) neurons but absent in non-dopaminergic neurons. The number of bodies varied from 0 to 6 per cell nucleus. Nucleophosmin (B23) was found in the neuronal nucleolus, with the nucleolus size being constant in the nigral neurons of each individual brain. All the observed neurons had only one large nucleolus with intense nucleophosmin immunoreactivity and a lightly stained region (1-2 m in diameter) that apparently represents the giant fibrillar center (GFC). An intensely immunostained nucleophosmin-containing granule was often observed at the GFC periphery. Double labeling demonstrated that nucleophosmin-immunoreactive nucleolus and ubiquitin-immunoreactive Marinesco bodies can occur both closely to and remotely from each other. Three-dimensional reconstruction indicates that rounded Marinesco bodies are polymorphic and often have a complex shape, with some flattening and concavities, which may be associated with contact not only with the nucleolus, but also, presumably, with other intranuclear structures free of ubiquitin or nucleophosmin. Ubiquitin-immunoreactive structures with a relatively small size (up to 1 m in length) and various clastosome-like shapes (Lafarga et al., 2002) often occur near Marinesco bodies. There were no cases of detection of ubiquitin in the nucleoli of dopaminergic neurons and nucleophosmin/B23 in typical Marinesco bodies. The obtained information may be helpful in unraveling the molecular mechanisms of the selective vulnerability of substantia nigra dopaminergic neurons to damaging factors.

scholarly journals Substantia Nigra Marinesco Bodies Are Associated with Decreased Striatal Expression of Dopaminergic Markers

2004 ◽  
Vol 63 (4) ◽  
pp. 329-337 ◽  
Author(s):  
Thomas G. Beach ◽  
Douglas G. Walker ◽  
Lucia I. Sue ◽  
Amanda Newell ◽  
Charles C. Adler ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Marinesco Bodies

scholarly journals Marinesco bodies and substantia nigra neuron density in Parkinson's disease

2017 ◽  
Vol 43 (7) ◽  
pp. 621-630 ◽  
Author(s):  
R. D. Abbott ◽  
J. S. Nelson ◽  
G.W. Ross ◽  
J. H. Uyehara-Lock ◽  
C. M. Tanner ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Substantia Nigra ◽  
Neuron Density ◽  
Substantia Nigra Neuron ◽  
Marinesco Bodies

Immunocytochemical methodology for the localization of neuronal antigens at the ultrastructural level

1990 ◽  
Vol 48 (3) ◽  
pp. 876-877
Author(s):  
Jorge Pecci Saavedra ◽  
Mark Connaughton ◽  
Juan José López ◽  
Alicia Brusco
Keyword(s):  
Spinal Cord ◽  
Substantia Nigra ◽  
Polyclonal Antibodies ◽  
Ultrastructural Level ◽  
Point Of View ◽  
Nerve Tissue ◽  
Tissue Fixation ◽  
Optimal Conditions ◽  
Interpeduncular Nucleus ◽  
Technical Point

The use of antibodies as labels for the localization of specific molecules in the nervous systan has been extensively applied in recent years. Both monoand polyclonal antibodies or antisera have been employed. The knowledge of the organization of neuronal connectivities, gliovascular relationships, glioneuronal relationships and other features of nerve tissue has greatly increased.A number of areas of the nervous systan have been analyzed in our laboratory, including the nuclei of the raphe system, the reticular formation, interpeduncular nucleus, substantia nigra, caudate nucleus, putamen, pallidum, spinal cord, pineal gland and others.From a technical point of view, a number of variables needed to be taken into account in order to obtain reliable and reproducible results. The design of the optimal conditions of tissue fixation, embedding, sectioning, dilution of antibodies, and adaptation of Sternberger PAP technique were sane of the parameters taken into account to optimize the results. It is critical that each step of the technique be defined for each particular case.

Diagnostic evaluation of the substantia nigra

1996 ◽  
Vol 22 (3) ◽  
pp. 228-232 ◽  
Author(s):  
D. A. McRitchie ◽  
G. M. Halliday ◽  
R. Pamphlett
Keyword(s):  
Substantia Nigra ◽  
Diagnostic Evaluation
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