scholarly journals Should they stay or should they go? Nitric oxide and the clash of regulators governing Vibrio fischeri biofilm formation

2018 ◽  
Author(s):  
Eric V. Stabb
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Vibrio Fischeri

scholarly journals Nitric oxide inhibits biofilm formation by Vibrio fischeri via the nitric oxide sensor HnoX

2018 ◽  
Vol 111 (1) ◽  
pp. 187-203 ◽  
Author(s):  
Cecilia M. Thompson ◽  
Alice H. Tischler ◽  
Denise A. Tarnowski ◽  
Mark J. Mandel ◽  
Karen L. Visick
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Vibrio Fischeri ◽  
Nitric Oxide Sensor

scholarly journals Effect of Nitroxides on Swarming Motility and Biofilm Formation, Multicellular Behaviors in Pseudomonas aeruginosa

2013 ◽  
Vol 57 (10) ◽  
pp. 4877-4881 ◽  
Author(s):  
César de la Fuente-Núñez ◽  
Fany Reffuveille ◽  
Kathryn E. Fairfull-Smith ◽  
Robert E. W. Hancock
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Wild Type ◽  
Planktonic Cells ◽  
Swarming Motility ◽  
Content Type ◽  
Exogenous Addition ◽  
Biofilm Dispersion ◽  
Dispersal Activity

ABSTRACTThe ability of nitric oxide (NO) to induce biofilm dispersion has been well established. Here, we investigated the effect of nitroxides (sterically hindered nitric oxide analogues) on biofilm formation and swarming motility inPseudomonas aeruginosa. A transposon mutant unable to produce nitric oxide endogenously (nirS) was deficient in swarming motility relative to the wild type and the complemented strain. Moreover, expression of thenirSgene was upregulated by 9.65-fold in wild-type swarming cells compared to planktonic cells. Wild-type swarming levels were substantially restored upon the exogenous addition of nitroxide containing compounds, a finding consistent with the hypothesis that NO is necessary for swarming motility. Here, we showed that nitroxides not only mimicked the dispersal activity of NO but also prevented biofilms from forming in flow cell chambers. In addition, anirStransposon mutant was deficient in biofilm formation relative to the wild type and the complemented strain, thus implicating NO in the formation of biofilms. Intriguingly, despite its stand-alone action in inhibiting biofilm formation and promoting dispersal, a nitroxide partially restored the ability of anirSmutant to form biofilms.

Facile solvent-free fabrication of nitric oxide (NO)-releasing coatings for prevention of biofilm formation

2017 ◽  
Vol 53 (48) ◽  
pp. 6488-6491 ◽  
Author(s):  
Kitty K. K. Ho ◽  
Berkay Ozcelik ◽  
Mark D. P. Willcox ◽  
Helmut Thissen ◽  
Naresh Kumar
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Solvent Free

A simple solvent-free method to generate nitric oxide (NO)-releasing coatings for prevention of adhesion and biofilm formation of common pathogens.

scholarly journals Tools for Rapid Genetic Engineering of Vibrio fischeri

2018 ◽  
Vol 84 (14) ◽  
Author(s):  
Karen L. Visick ◽  
Kelsey M. Hodge-Hanson ◽  
Alice H. Tischler ◽  
Allison K. Bennett ◽  
Vincent Mastrodomenico
Keyword(s):  
Antibiotic Resistance ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Fischeri ◽  
Single Step ◽  
Universal Primers ◽  
Resistance Marker ◽  
Content Type ◽  
Flp Recombinase ◽  
Overlap Extension

ABSTRACT Vibrio fischeri is used as a model for a number of processes, including symbiosis, quorum sensing, bioluminescence, and biofilm formation. Many of these studies depend on generating deletion mutants and complementing them. Engineering such strains, however, is a time-consuming, multistep process that relies on cloning and subcloning. Here, we describe a set of tools that can be used to rapidly engineer deletions and insertions in the V. fischeri chromosome without cloning. We developed a uniform approach for generating deletions using PCR splicing by overlap extension (SOEing) with antibiotic cassettes flanked by standardized linker sequences. PCR SOEing of the cassettes to sequences up- and downstream of the target gene generates a DNA product that can be directly introduced by natural transformation. Selection for the introduced antibiotic resistance marker yields the deletion of interest in a single step. Because these cassettes also contain FRT (FLP recognition target) sequences flanking the resistance marker, Flp recombinase can be used to generate an unmarked, in-frame deletion. We developed a similar methodology and tools for the rapid insertion of specific genes at a benign site in the chromosome for purposes such as complementation. Finally, we generated derivatives of these tools to facilitate different applications, such as inducible gene expression and assessing protein production. We demonstrated the utility of these tools by deleting and inserting genes known or predicted to be involved in motility. While developed for V. fischeri strain ES114, we anticipate that these tools can be adapted for use in other V. fischeri strains and, potentially, other microbes. IMPORTANCE Vibrio fischeri is a model organism for studying a variety of important processes, including symbiosis, biofilm formation, and quorum sensing. To facilitate investigation of these biological mechanisms, we developed approaches for rapidly generating deletions and insertions and demonstrated their utility using two genes of interest. The ease, consistency, and speed of the engineering is facilitated by a set of antibiotic resistance cassettes with common linker sequences that can be amplified by PCR with universal primers and fused to adjacent sequences using splicing by overlap extension and then introduced directly into V. fischeri , eliminating the need for cloning and plasmid conjugation. The antibiotic cassettes are flanked by FRT sequences, permitting their removal using Flp recombinase. We augmented these basic tools with a family of constructs for different applications. We anticipate that these tools will greatly accelerate mechanistic studies of biological processes in V. fischeri and potentially other Vibrio species.

scholarly journals The symbiosis regulator RscS controls the syp gene locus, biofilm formation and symbiotic aggregation by Vibrio fischeri

2006 ◽  
Vol 62 (6) ◽  
pp. 1586-1600 ◽  
Author(s):  
Emily S. Yip ◽  
Kati Geszvain ◽  
Cindy R. DeLoney-Marino ◽  
Karen L. Visick
Keyword(s):  
Biofilm Formation ◽  
Gene Locus ◽  
Vibrio Fischeri

Effect of exogenous and endogenous nitric oxide on biofilm formation by Lactobacillus plantarum

Microbiology ◽  
2013 ◽  
Vol 82 (4) ◽  
pp. 423-427 ◽  
Author(s):  
D. R. Yarullina ◽  
L. V. Vakatova ◽  
A. V. Krivoruchko ◽  
E. V. Rubtsova ◽  
O. N. Ilinskaya
Keyword(s):  
Nitric Oxide ◽  
Lactobacillus Plantarum ◽  
Biofilm Formation ◽  
Endogenous Nitric Oxide

scholarly journals Impact of Salt and Nutrient Content on Biofilm Formation by Vibrio fischeri

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169521 ◽  
Author(s):  
Anne E. Marsden ◽  
Kevin Grudzinski ◽  
Jakob M. Ondrey ◽  
Cindy R. DeLoney-Marino ◽  
Karen L. Visick
Keyword(s):  
Biofilm Formation ◽  
Vibrio Fischeri ◽  
Nutrient Content

scholarly journals Environmental and Genetic Determinants of Biofilm Formation inParacoccus denitrificans

mSphere ◽  
2017 ◽  
Vol 2 (5) ◽  
Author(s):  
Santosh Kumar ◽  
Stephen Spiro
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Paracoccus Denitrificans ◽  
Binding Protein ◽  
Anaerobic Growth ◽  
Type I ◽  
Content Type ◽  
Attached Growth ◽  
Polystyrene Surface ◽  
Biofilm Development

ABSTRACTThe genome of the denitrifying bacteriumParacoccus denitrificanspredicts the expression of a small heme-containing nitric oxide (NO) binding protein, H-NOX. The genome organization and prior work in other bacteria suggest that H-NOX interacts with a diguanylate cyclase that cyclizes GTP to make cyclic di-GMP (cdGMP). Since cdGMP frequently regulates attached growth as a biofilm, we first established conditions for biofilm development byP. denitrificans. We found that adhesion to a polystyrene surface is strongly stimulated by the addition of 10 mM Ca2+to rich media. The genome encodes at least 11 repeats-in-toxin family proteins that are predicted to be secreted by the type I secretion system (TISS). We deleted the genes encoding the TISS and found that the mutant is almost completely deficient for attached growth. Adjacent to the TISS genes there is a potential open reading frame encoding a 2,211-residue protein with 891 Asp-Ala repeats. This protein is also predicted to bind calcium and to be a TISS substrate, and a mutant specifically lacking this protein is deficient in biofilm formation. By analysis of mutants and promoter reporter fusions, we show that biofilm formation is stimulated by NO generated endogenously by the respiratory reduction of nitrite. A mutant lacking both predicted diguanylate cyclases encoded in the genome overproduces biofilm, implying that cdGMP is a negative regulator of attached growth. Our data are consistent with a model in which there are H-NOX-dependent and -independent pathways by which NO stimulates biofilm formation.IMPORTANCEThe bacteriumParacoccus denitrificansis a model for the process of denitrification, by which nitrate is reduced to dinitrogen during anaerobic growth. Denitrification is important for soil fertility and greenhouse gas emission and in waste and water treatment processes. The ability of bacteria to grow as a biofilm attached to a solid surface is important in many different contexts. In this paper, we report that attached growth ofP. denitrificansis stimulated by nitric oxide, an intermediate in the denitrification pathway. We also show that calcium ions stimulate attached growth, and we identify a large calcium binding protein that is required for growth on a polystyrene surface. We identify components of a signaling pathway through which nitric oxide may regulate biofilm formation. Our results point to an intimate link between metabolic processes and the ability ofP. denitrificansto grow attached to a surface.

Sign in / Sign up

Export Citation Format

Share Document