Facile solvent-free fabrication of nitric oxide (NO)-releasing coatings for prevention of biofilm formation

2017 ◽  
Vol 53 (48) ◽  
pp. 6488-6491 ◽  
Author(s):  
Kitty K. K. Ho ◽  
Berkay Ozcelik ◽  
Mark D. P. Willcox ◽  
Helmut Thissen ◽  
Naresh Kumar
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Solvent Free

A simple solvent-free method to generate nitric oxide (NO)-releasing coatings for prevention of adhesion and biofilm formation of common pathogens.

scholarly journals Effect of Nitroxides on Swarming Motility and Biofilm Formation, Multicellular Behaviors in Pseudomonas aeruginosa

2013 ◽  
Vol 57 (10) ◽  
pp. 4877-4881 ◽  
Author(s):  
César de la Fuente-Núñez ◽  
Fany Reffuveille ◽  
Kathryn E. Fairfull-Smith ◽  
Robert E. W. Hancock
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Wild Type ◽  
Planktonic Cells ◽  
Swarming Motility ◽  
Content Type ◽  
Exogenous Addition ◽  
Biofilm Dispersion ◽  
Dispersal Activity

ABSTRACTThe ability of nitric oxide (NO) to induce biofilm dispersion has been well established. Here, we investigated the effect of nitroxides (sterically hindered nitric oxide analogues) on biofilm formation and swarming motility inPseudomonas aeruginosa. A transposon mutant unable to produce nitric oxide endogenously (nirS) was deficient in swarming motility relative to the wild type and the complemented strain. Moreover, expression of thenirSgene was upregulated by 9.65-fold in wild-type swarming cells compared to planktonic cells. Wild-type swarming levels were substantially restored upon the exogenous addition of nitroxide containing compounds, a finding consistent with the hypothesis that NO is necessary for swarming motility. Here, we showed that nitroxides not only mimicked the dispersal activity of NO but also prevented biofilms from forming in flow cell chambers. In addition, anirStransposon mutant was deficient in biofilm formation relative to the wild type and the complemented strain, thus implicating NO in the formation of biofilms. Intriguingly, despite its stand-alone action in inhibiting biofilm formation and promoting dispersal, a nitroxide partially restored the ability of anirSmutant to form biofilms.

Effect of exogenous and endogenous nitric oxide on biofilm formation by Lactobacillus plantarum

Microbiology ◽  
2013 ◽  
Vol 82 (4) ◽  
pp. 423-427 ◽  
Author(s):  
D. R. Yarullina ◽  
L. V. Vakatova ◽  
A. V. Krivoruchko ◽  
E. V. Rubtsova ◽  
O. N. Ilinskaya
Keyword(s):  
Nitric Oxide ◽  
Lactobacillus Plantarum ◽  
Biofilm Formation ◽  
Endogenous Nitric Oxide

scholarly journals Environmental and Genetic Determinants of Biofilm Formation inParacoccus denitrificans

mSphere ◽  
2017 ◽  
Vol 2 (5) ◽  
Author(s):  
Santosh Kumar ◽  
Stephen Spiro
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Paracoccus Denitrificans ◽  
Binding Protein ◽  
Anaerobic Growth ◽  
Type I ◽  
Content Type ◽  
Attached Growth ◽  
Polystyrene Surface ◽  
Biofilm Development

ABSTRACTThe genome of the denitrifying bacteriumParacoccus denitrificanspredicts the expression of a small heme-containing nitric oxide (NO) binding protein, H-NOX. The genome organization and prior work in other bacteria suggest that H-NOX interacts with a diguanylate cyclase that cyclizes GTP to make cyclic di-GMP (cdGMP). Since cdGMP frequently regulates attached growth as a biofilm, we first established conditions for biofilm development byP. denitrificans. We found that adhesion to a polystyrene surface is strongly stimulated by the addition of 10 mM Ca2+to rich media. The genome encodes at least 11 repeats-in-toxin family proteins that are predicted to be secreted by the type I secretion system (TISS). We deleted the genes encoding the TISS and found that the mutant is almost completely deficient for attached growth. Adjacent to the TISS genes there is a potential open reading frame encoding a 2,211-residue protein with 891 Asp-Ala repeats. This protein is also predicted to bind calcium and to be a TISS substrate, and a mutant specifically lacking this protein is deficient in biofilm formation. By analysis of mutants and promoter reporter fusions, we show that biofilm formation is stimulated by NO generated endogenously by the respiratory reduction of nitrite. A mutant lacking both predicted diguanylate cyclases encoded in the genome overproduces biofilm, implying that cdGMP is a negative regulator of attached growth. Our data are consistent with a model in which there are H-NOX-dependent and -independent pathways by which NO stimulates biofilm formation.IMPORTANCEThe bacteriumParacoccus denitrificansis a model for the process of denitrification, by which nitrate is reduced to dinitrogen during anaerobic growth. Denitrification is important for soil fertility and greenhouse gas emission and in waste and water treatment processes. The ability of bacteria to grow as a biofilm attached to a solid surface is important in many different contexts. In this paper, we report that attached growth ofP. denitrificansis stimulated by nitric oxide, an intermediate in the denitrification pathway. We also show that calcium ions stimulate attached growth, and we identify a large calcium binding protein that is required for growth on a polystyrene surface. We identify components of a signaling pathway through which nitric oxide may regulate biofilm formation. Our results point to an intimate link between metabolic processes and the ability ofP. denitrificansto grow attached to a surface.

scholarly journals Efficacy of surface-generated nitric oxide againstCandida albicansadhesion and biofilm formation

Biofouling ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 973-983 ◽  
Author(s):  
Benjamin J. Privett ◽  
Steven T. Nutz ◽  
Mark H. Schoenfisch
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation

scholarly journals Anaerobic Metabolism Occurs in the Substratum of Gonococcal Biofilms and May Be Sustained in Part by Nitric Oxide

2010 ◽  
Vol 78 (5) ◽  
pp. 2320-2328 ◽  
Author(s):  
Megan L. Falsetta ◽  
Alastair G. McEwan ◽  
Michael P. Jennings ◽  
Michael A. Apicella
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Biofilm Formation ◽  
Chronic Infection ◽  
Anaerobic Metabolism ◽  
Anaerobic Respiration ◽  
The United States ◽  
Anaerobic Growth ◽  
Insertion Mutant ◽  
Biofilm Development

ABSTRACT Neisseria gonorrhoeae is the etiologic agent of gonorrhea, which has been among the most frequently reported communicable diseases in the United States since 1960. Women frequently do not exhibit symptoms, which can lead to chronic infection. N. gonorrhoeae readily forms biofilms over abiotic surfaces, over primary and transformed cervical epithelial cells, and over cervical tissues in vivo. Biofilms are often associated with chronic infection, which suggests a link between biofilm formation and asymptomatic gonorrhea in women. Proteins involved in anaerobic metabolism and oxidative-stress tolerance are critical for normal biofilm formation of N. gonorrhoeae. Therefore, we examined the spatial profiles of anaerobic respiration in N. gonorrhoeae, using an aniA′-′gfp transcriptional fusion. Nitric oxide (NO) can elicit biofilm dispersal when present at sublethal concentrations in the surrounding medium. Some reports indicate that NO may also encourage biofilm formation at higher, potentially lethal concentrations. NO is produced by polymorphonuclear lymphocytes (PMNs) and cervical endothelial and epithelial cells. Thus, we also examined the effect of NO on N. gonorrhoeae biofilms. We found that anaerobic respiration occurs predominantly in the substratum of gonococcal biofilms and that expression of aniA is induced over time in biofilms. Treatment with high concentrations of a rapid-release NO donor prevents biofilm formation when supplied early in biofilm development but can also enhance biofilm formation once anaerobic respiration is initiated. NO treatment partially restores biofilm formation in an aniA::kan insertion mutant, which suggests that N. gonorrhoeae in biofilms may use NO as a substrate for anaerobic growth but prefer nitrite.

scholarly journals Aspergillus nidulans biofilm formation modifies cellular architecture and enables light-activated autophagy

2021 ◽  
pp. mbc.E20-11-0734
Author(s):  
Dale E. Lingo ◽  
Nandini Shukla ◽  
Aysha H. Osmani ◽  
Stephen A. Osmani
Keyword(s):  
Nitric Oxide ◽  
Drug Resistance ◽  
Transcription Factor ◽  
Aspergillus Nidulans ◽  
Biofilm Formation ◽  
Cell Biology ◽  
Antifungal Drug Resistance ◽  
Cellular Architecture ◽  
Er Exit Sites ◽  
Spinning Disc

After growing on surfaces, including those of medical and industrial importance, fungal biofilms self-generate internal microenvironments. We previously reported that gaseous microenvironments around founder Aspergillus nidulans cells change during biofilm formation causing microtubules (MTs) to disassemble under control of the hypoxic transcription factor SrbA. Here we investigate if biofilm formation might also promote changes to structures involved in exocytosis and endocytosis. During biofilm formation the ER remained intact but ER exit sites and the Golgi apparatus were modified as were endocytic actin patches. The biofilm driven changes required the SrbA hypoxic transcription factor and could be triggered by nitric oxide, further implicating gaseous regulation of biofilm cellular architecture. By tracking GFP-Atg8 dynamics, biofilm founder cells were also observed to undergo autophagy. Most notably, biofilm cells that had undergone autophagy were triggered into further autophagy by spinning disc confocal light. Our findings indicate that fungal biofilm formation modifies the secretory and endocytic apparatus and show biofilm cells can also undergo autophagy that is reactivated by light. The findings provide new insights into the changes occurring in fungal biofilm cell biology that potentially impact their unique characteristics, including antifungal drug resistance. [Media: see text]

scholarly journals Oxygen depletion and nitric oxide stimulate type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA

2021 ◽  
Author(s):  
Hannah Q Hughes ◽  
Kyle A Floyd ◽  
Sajjad Hossain ◽  
Sweta Anantharaman ◽  
David T Kysela ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vibrio Cholerae ◽  
Biofilm Formation ◽  
Bacterial Species ◽  
Bacterial Pathogen ◽  
Dna Uptake ◽  
Dynamic Activity ◽  
Regulatory Pathways ◽  
Type Iv ◽  
Microaerobic Conditions

Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment to surfaces. Dynamic extension and retraction of pili is often required for these activities, but the stimuli that regulate these dynamics remain poorly characterized. To study this question, we use the bacterial pathogen Vibrio cholerae, which uses mannose-sensitive hemagglutinin (MSHA) pili to attach to surfaces in aquatic environments as the first step in biofilm formation. Here, we find that V. cholerae cells retract MSHA pili and detach from a surface in microaerobic conditions. This response is dependent on the phosphodiesterase CdpA, which decreases intracellular levels of cyclic-di-GMP (c-di-GMP) under microaerobic conditions to induce MSHA pilus retraction. CdpA contains a putative NO-sensing NosP domain, and we demonstrate that nitric oxide (NO) is necessary and sufficient to stimulate CdpA-dependent detachment. Thus, we hypothesize that microaerobic conditions result in endogenous production of NO (or an NO-like molecule) in V. cholerae. Together, these results describe a regulatory pathway that allows V. cholerae to rapidly abort biofilm formation. More broadly, these results show how environmental cues can be integrated into the complex regulatory pathways that control pilus dynamic activity and attachment in bacterial species.

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