scholarly journals Nitric oxide inhibits biofilm formation by Vibrio fischeri via the nitric oxide sensor HnoX

2018 ◽  
Vol 111 (1) ◽  
pp. 187-203 ◽  
Author(s):  
Cecilia M. Thompson ◽  
Alice H. Tischler ◽  
Denise A. Tarnowski ◽  
Mark J. Mandel ◽  
Karen L. Visick
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Vibrio Fischeri ◽  
Nitric Oxide Sensor

A Simple Silicon Based Nitric Oxide Sensor

1999 ◽  
Author(s):  
Marcelo Bariatto ◽  
Rogerio Furlan ◽  
Koiti Arakai ◽  
Jorge J. Santiago-Aviles
Keyword(s):  
Nitric Oxide ◽  
Amperometric Detection ◽  
Cellular Level ◽  
High Yield ◽  
Sensor Calibration ◽  
Nitric Oxide Sensor ◽  
Sensor Configuration

Abstract Nitric oxide (NO) is known to mediate many beneficial physiology processes, motivating its detection in vivo as well as in vitro. Electrochemical detection provides the required cellular level determination of NO among several other techniques. In this work, electrochemical micro-sensors for both types of detection, in vivo and in vitro, were developed, exploring the silicon planar technology, which presents high yield and reliability and also permits batch fabrication. The developed in vitro sensor features eight detection sites (10 μm × 10 μm microelectrodes), for determination of nitric oxide spatial distribution or multi-species analysis. Different electrochemical methods were applied to provide sensor calibration and chemical reproducibility. For in vivo analysis, the designed structures have a needle shape (40 μm thick) and they were silicon micro-machined by using plasma etching or etch stop techniques. Different configurations were designed and implemented, containing a number of detection microelectrodes that vary from 2 to 10. The amperometric detection of both nitric oxide and nitride (NO2−) — a molecule that causes an interference — were investigated by using the in vitro micro-sensor configuration. The need of a cationic exchanger (Nafion) was demonstrated in order to provide selectivity to NO for low concentrations. Also, the developed sensor has a sensitivity of 500 A/M.cm2 and a detection limit of 10 μM.

scholarly journals Novel Solid State Nitric Oxide Sensor Using Siloxane-Poly(Oxypropylene) (PPO)

2013 ◽  
Vol 04 (11) ◽  
pp. 683-688 ◽  
Author(s):  
Rondinelli D. Herculano ◽  
Carlos A. Brunello ◽  
Jair P. Melo Jr. ◽  
Mayler Martins ◽  
Felipe A. Borges ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Solid State ◽  
Nitric Oxide Sensor

scholarly journals Effect of Nitroxides on Swarming Motility and Biofilm Formation, Multicellular Behaviors in Pseudomonas aeruginosa

2013 ◽  
Vol 57 (10) ◽  
pp. 4877-4881 ◽  
Author(s):  
César de la Fuente-Núñez ◽  
Fany Reffuveille ◽  
Kathryn E. Fairfull-Smith ◽  
Robert E. W. Hancock
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Wild Type ◽  
Planktonic Cells ◽  
Swarming Motility ◽  
Content Type ◽  
Exogenous Addition ◽  
Biofilm Dispersion ◽  
Dispersal Activity

ABSTRACTThe ability of nitric oxide (NO) to induce biofilm dispersion has been well established. Here, we investigated the effect of nitroxides (sterically hindered nitric oxide analogues) on biofilm formation and swarming motility inPseudomonas aeruginosa. A transposon mutant unable to produce nitric oxide endogenously (nirS) was deficient in swarming motility relative to the wild type and the complemented strain. Moreover, expression of thenirSgene was upregulated by 9.65-fold in wild-type swarming cells compared to planktonic cells. Wild-type swarming levels were substantially restored upon the exogenous addition of nitroxide containing compounds, a finding consistent with the hypothesis that NO is necessary for swarming motility. Here, we showed that nitroxides not only mimicked the dispersal activity of NO but also prevented biofilms from forming in flow cell chambers. In addition, anirStransposon mutant was deficient in biofilm formation relative to the wild type and the complemented strain, thus implicating NO in the formation of biofilms. Intriguingly, despite its stand-alone action in inhibiting biofilm formation and promoting dispersal, a nitroxide partially restored the ability of anirSmutant to form biofilms.

Facile solvent-free fabrication of nitric oxide (NO)-releasing coatings for prevention of biofilm formation

2017 ◽  
Vol 53 (48) ◽  
pp. 6488-6491 ◽  
Author(s):  
Kitty K. K. Ho ◽  
Berkay Ozcelik ◽  
Mark D. P. Willcox ◽  
Helmut Thissen ◽  
Naresh Kumar
Keyword(s):  
Nitric Oxide ◽  
Biofilm Formation ◽  
Solvent Free

A simple solvent-free method to generate nitric oxide (NO)-releasing coatings for prevention of adhesion and biofilm formation of common pathogens.

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