scholarly journals In vitro assessment of quality of citrate-phosphate-dextrose-adenine-1 preserved feline blood collected by a commercial closed system

2018 ◽  
Vol 32 (3) ◽  
pp. 1051-1059 ◽  
Author(s):  
Chiara Crestani ◽  
Annalisa Stefani ◽  
Antonio Carminato ◽  
Angelica Cro ◽  
Katia Capello ◽  
...  
Keyword(s):  
Closed System ◽  
Citrate Phosphate Dextrose ◽  
In Vitro Assessment ◽  
Citrate Phosphate

Measurement of red cell sickling: a method for studying the efficacy of antisickling drugs under physiological conditions

1983 ◽  
Vol 61 (8) ◽  
pp. 941-945 ◽  
Author(s):  
S. Tsuyoshi Ohnishi ◽  
Koji Hashimoto ◽  
Takashi Sato
Keyword(s):  
Cell Morphology ◽  
Light Microscope ◽  
Optical Path ◽  
Gas Mixture ◽  
Red Cell ◽  
Physiological Conditions ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

A method was developed to study sickling in vitro under physiological conditions using a small amount of blood (0.1 mL). The diluted blood suspension (2.1 mL) was placed in a flask and flushed with a gas mixture containing 5% CO2. In deoxygenation experiments, samples were withdrawn anaerobically into a microslide (optical path 0.1 mm) and red cell morphology was studied directly under a light microscope after both ends of the microslide were sealed. The blood suspension with a hematocrit value of 1% can be deoxygenated in less than 10 min, but it takes 30 min for the sickling of cells to reach a plateau. The degree of sickling increases with increasing osmolality of the medium, or with a decrease of the pH. With a citrate–phosphate–dextrose–adenine solution, sickle blood may be stored for this type of study for about 10 days at 4 °C. The blood may be stored for about 5 days with a noncitrate preservative. This method was found useful in examining the antisickling activity of various drugs.

Comparative evaluation of the in-vitro viability of canine and human blood preserved in citrate phosphate dextrose adenine (CPDA)-1 anticoagulated blood bag

2019 ◽  
Author(s):  
R. I. Udegbunam ◽  
C. S. Njaka ◽  
H. N. Okereke ◽  
S. O. Udegbunam
Keyword(s):  
Human Blood ◽  
Membrane Damage ◽  
Plasma Potassium ◽  
Progressive Increase ◽  
Ion Concentration ◽  
Percentage Decrease ◽  
Citrate Phosphate Dextrose ◽  
Blood Bag ◽  
Citrate Phosphate

Two hundred and fifty millilitres of blood each were drawn from healthy dogs (n=3) and volunteer human donors (n=3) into citrate phosphate dextrose adenine -1 anti-coagulated blood bags and preserved for 21 days. On days 0, 3, 7, 14 and 21, selected hematological and biochemical parameters were evaluated. Red blood cells counts of canine and human blood showed no significant (p>0.05) difference till days 14 and 21 respectively. Mean corpuscular value (MCV) of canine blood on day 21 was significantly (p less than 0.05) higher than that of human blood. Erythrocyte catalase (CAT) and glutathione (GSH) progressively decreased while plasma potassium ion concentration of canine and human blood progressively increased. On day 21, percentage decrease in canine RBC antioxidants was significantly higher when compared with that of human blood. The progressive decrease in RBC’s CAT and GSH suggests increased oxidative stress while progressive increase in K+ concentration and MCV suggests RBC membrane damage.

In Vitro Assessment of the Quality of Stored Platelet Concentrates

1994 ◽  
Vol 8 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Scott Murphy ◽  
Paulo Rebulla ◽  
Francesco Bertolini ◽  
Stein Holme ◽  
Gary Moroff ◽  
...  
Keyword(s):  
Platelet Concentrates ◽  
In Vitro Assessment

scholarly journals Mechanical Strength Variety of Orthodontic Polymeric Chains In vitro assessment and mathematical model

2019 ◽  
Vol 56 (3) ◽  
pp. 596-599
Author(s):  
Olimpia Bunta ◽  
Vlad Muresan ◽  
Mariana Pacurar ◽  
Mihaela Baciut ◽  
Dorin Nenovici ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Direct Impact ◽  
Force Decay ◽  
Polymeric Chains ◽  
In Vitro Assessment ◽  
Manufacturing Techniques ◽  
Initial Force ◽  
Elastomeric Chains

Elastomeric chains, as means of force delivery, are one of the most studied elements of the orthodontic field. Effects on tooth movement are closely related to force degradation degrees of these polymers. Unfortunately, due to varied manufacturing techniques and materials, different brands of elastomeric chains offer different initial force values and different force decay, with direct impact on tooth movement duration and effectiveness and quality of orthodontic treatments.

scholarly journals IN VITRO ASSESSMENT OF PHYSICOCHEMICAL PARAMETERS OF FIVE GENERICS AMLODIPINE BESYLATE TABLETS MARKETED IN YEMEN

2021 ◽  
pp. 15-19
Author(s):  
ARWA ALSHARGABI
Keyword(s):  
Physicochemical Parameters ◽  
Dissolution Test ◽  
Amlodipine Besylate ◽  
Disintegration Time ◽  
Physiochemical Parameters ◽  
Weight Variation ◽  
Label Claim ◽  
In Vitro Assessment

Objective: The present paper aims to evaluate the quality of five different brands (local and imported) of oral film-coated tablets of generic Amlodipine besylate 5 mg marketed in Sana`a-Yemen, through physiochemical parameters. Methods: Different physicochemical parameters, including the uniformity of tablet weight, hardness, thickness, disintegration time, and an assay of active ingredients, were conducted to validate the quality of generics Amlodipine Besylate 5 mg according to USP specification. Results: From the obtained results, it was observed that all the brands of Amlodipine Besylate 5 mg have passed the tests and met the specifications of USP. Results of weight variation, hardness, thickness, and disintegration time were ranged from-3.8 % to+5.13 % to-1.25 % to+3.25 %, 5.06±0.31 to 13.21±1.5, 2.682±0.04 to 3.676±0.01 and 25 s to 2 min: 30 s, respectively. The dissolution test and the assay results of all the brands are also ranged within the acceptable label claim 93.7±2.24 to 98.4±0.85 and 93.22±0.38 to 100.15±0.33, respectively. However, there is no relation was found between the disintegration time and the dissolution test. Conclusion: According to the finding, all the selected Amlodipine Besylate 5 mg brands are met pharmacopeia standards and USP specifications. Therefore, the local and imported Amlodipine Besylate 5 mg can be used safely to get the desired therapeutic efficiency.

scholarly journals Predicting in vivo starch digestibility coefficients in newly weaned piglets from in vitro assessment of diets using multivariate analysis

2009 ◽  
Vol 103 (9) ◽  
pp. 1309-1318 ◽  
Author(s):  
Frederic J. Doucet ◽  
Gavin A. White ◽  
Florian Wulfert ◽  
Sandra E. Hill ◽  
Julian Wiseman
Keyword(s):  
Water Solubility ◽  
Starch Digestibility ◽  
Degree Of Crystallinity ◽  
Starch Granules ◽  
Weaned Piglets ◽  
In Vitro Assessment ◽  
Small Intestinal

The study was based on correlating a dataset of in vivo mean starch digestibility coefficients obtained in the immediate post-weaning phase of piglets with a range of dietary in vitro variables. The paper presents a model that predicts (R2 0·71) in vivo average starch digestibility coefficients in the 0·5 small-intestinal region of newly weaned piglets fed cereal-based diets using seven in vitro variables describing starch properties that are fundamentally associated with the quality of feed materials, i.e. hydration, structure and amylolytic digestion. The variables were: Rapid Visco Analyser (RVA; measures the viscosity of materials when sheared under defined hydration and temperature regimens); RVA end viscosity; RVA (gelatinisation) peak viscosity; ΔH (gelatinisation enthalpy that provides an estimate of helical order or degree of crystallinity in starch); water solubility index (WSI; that denotes the amount of soluble polysaccharides released from starch granules to the aqueous phase); grain endogenous amylase (concentration of endogenous α-amylase in cereals, assessed by pasting cereal flours in 25 g of AgNO3, an amylase inhibitor v. water using RVA).

scholarly journals In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant

2020 ◽  
Vol 13 (4) ◽  
pp. 726-730
Author(s):  
Okereke Henry Nnamdi ◽  
Udegbunam Rita Ijeoma ◽  
Nwobi Lotanna Gilbert ◽  
Ezeobialu Henry Toochukwu ◽  
Udegbunam Sunday Ositadinma
Keyword(s):  
Oxidative Stress ◽  
Antioxidant System ◽  
Blood Cells ◽  
Protective Capacity ◽  
Citrate Phosphate Dextrose ◽  
Protective System ◽  
Primary Antioxidant ◽  
Antioxidant Markers ◽  
Citrate Phosphate

Background and Aim: Stored blood is continuously exposed to oxidative stress, which affects its antioxidant protective system. Erythrocytes are naturally armed with antioxidant protective capacity. Blood antioxidant system functions to protect the blood cells against oxidative damage by free radicals. However, during storage, blood is continuously exposed to oxidative stress, which affects its antioxidant system. The aim of this work was to investigate alteration in malondialdehyde (MDA) levels, reduced glutathione (glutathione reductase [GSH-Rd]), catalase (CAT), and superoxide dismutase (SOD) activities in stored donkey blood. Materials and Methods: Blood (250 ml) was drawn from four clinically healthy donkeys into citrate phosphate dextrose adenine 1 blood bags and preserved at 4°C. MDA, GSH-Rd, CAT, and SOD activities were assayed by colorimetric methods, over a period of 42 days. Results: The result showed that SOD enzyme activities significantly (p<0.05) increased by day 7 post-storage (PS) while MDA levels significantly (p<0.05) increased by day 21 PS. However, activities of GSH-Rd and CAT enzymes decreased (p<0.05) by day 21 PS. Pearson's product-moment correlation showed a negative correlation between the levels of MDA and enzymatic antioxidant markers (CAT and GSH-Rd). Conclusion: The findings revealed that GSH-Rd and CAT are the primary antioxidant defense markers in donkey red blood cells. The observed alterations in these principal antioxidants suggest a 14 days optimum keeping time of donkey blood for blood banking purposes.

scholarly journals In-vitro Assessment of the Antibacterial Quality of Some Commonly Used Herbal and Non-herbal Toothpastes on Streptococcus mutans

2016 ◽  
Vol 4 (2) ◽  
pp. 21-25
Author(s):  
Awah N. S. ◽  
Agu K. C. ◽  
Okorie C. C. ◽  
Okeke C. B. ◽  
Iloanusi C. A. ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Assessment
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