Measurement of red cell sickling: a method for studying the efficacy of antisickling drugs under physiological conditions

1983 ◽  
Vol 61 (8) ◽  
pp. 941-945 ◽  
Author(s):  
S. Tsuyoshi Ohnishi ◽  
Koji Hashimoto ◽  
Takashi Sato
Keyword(s):  
Cell Morphology ◽  
Light Microscope ◽  
Optical Path ◽  
Gas Mixture ◽  
Red Cell ◽  
Physiological Conditions ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

A method was developed to study sickling in vitro under physiological conditions using a small amount of blood (0.1 mL). The diluted blood suspension (2.1 mL) was placed in a flask and flushed with a gas mixture containing 5% CO2. In deoxygenation experiments, samples were withdrawn anaerobically into a microslide (optical path 0.1 mm) and red cell morphology was studied directly under a light microscope after both ends of the microslide were sealed. The blood suspension with a hematocrit value of 1% can be deoxygenated in less than 10 min, but it takes 30 min for the sickling of cells to reach a plateau. The degree of sickling increases with increasing osmolality of the medium, or with a decrease of the pH. With a citrate–phosphate–dextrose–adenine solution, sickle blood may be stored for this type of study for about 10 days at 4 °C. The blood may be stored for about 5 days with a noncitrate preservative. This method was found useful in examining the antisickling activity of various drugs.

Comparative evaluation of the in-vitro viability of canine and human blood preserved in citrate phosphate dextrose adenine (CPDA)-1 anticoagulated blood bag

2019 ◽  
Author(s):  
R. I. Udegbunam ◽  
C. S. Njaka ◽  
H. N. Okereke ◽  
S. O. Udegbunam
Keyword(s):  
Human Blood ◽  
Membrane Damage ◽  
Plasma Potassium ◽  
Progressive Increase ◽  
Ion Concentration ◽  
Percentage Decrease ◽  
Citrate Phosphate Dextrose ◽  
Blood Bag ◽  
Citrate Phosphate

Two hundred and fifty millilitres of blood each were drawn from healthy dogs (n=3) and volunteer human donors (n=3) into citrate phosphate dextrose adenine -1 anti-coagulated blood bags and preserved for 21 days. On days 0, 3, 7, 14 and 21, selected hematological and biochemical parameters were evaluated. Red blood cells counts of canine and human blood showed no significant (p>0.05) difference till days 14 and 21 respectively. Mean corpuscular value (MCV) of canine blood on day 21 was significantly (p less than 0.05) higher than that of human blood. Erythrocyte catalase (CAT) and glutathione (GSH) progressively decreased while plasma potassium ion concentration of canine and human blood progressively increased. On day 21, percentage decrease in canine RBC antioxidants was significantly higher when compared with that of human blood. The progressive decrease in RBC’s CAT and GSH suggests increased oxidative stress while progressive increase in K+ concentration and MCV suggests RBC membrane damage.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284 ◽  
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals In vitro assessment of quality of citrate-phosphate-dextrose-adenine-1 preserved feline blood collected by a commercial closed system

2018 ◽  
Vol 32 (3) ◽  
pp. 1051-1059 ◽  
Author(s):  
Chiara Crestani ◽  
Annalisa Stefani ◽  
Antonio Carminato ◽  
Angelica Cro ◽  
Katia Capello ◽  
...  
Keyword(s):  
Closed System ◽  
Citrate Phosphate Dextrose ◽  
In Vitro Assessment ◽  
Citrate Phosphate

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant

2020 ◽  
Vol 13 (4) ◽  
pp. 726-730
Author(s):  
Okereke Henry Nnamdi ◽  
Udegbunam Rita Ijeoma ◽  
Nwobi Lotanna Gilbert ◽  
Ezeobialu Henry Toochukwu ◽  
Udegbunam Sunday Ositadinma
Keyword(s):  
Oxidative Stress ◽  
Antioxidant System ◽  
Blood Cells ◽  
Protective Capacity ◽  
Citrate Phosphate Dextrose ◽  
Protective System ◽  
Primary Antioxidant ◽  
Antioxidant Markers ◽  
Citrate Phosphate

Background and Aim: Stored blood is continuously exposed to oxidative stress, which affects its antioxidant protective system. Erythrocytes are naturally armed with antioxidant protective capacity. Blood antioxidant system functions to protect the blood cells against oxidative damage by free radicals. However, during storage, blood is continuously exposed to oxidative stress, which affects its antioxidant system. The aim of this work was to investigate alteration in malondialdehyde (MDA) levels, reduced glutathione (glutathione reductase [GSH-Rd]), catalase (CAT), and superoxide dismutase (SOD) activities in stored donkey blood. Materials and Methods: Blood (250 ml) was drawn from four clinically healthy donkeys into citrate phosphate dextrose adenine 1 blood bags and preserved at 4°C. MDA, GSH-Rd, CAT, and SOD activities were assayed by colorimetric methods, over a period of 42 days. Results: The result showed that SOD enzyme activities significantly (p<0.05) increased by day 7 post-storage (PS) while MDA levels significantly (p<0.05) increased by day 21 PS. However, activities of GSH-Rd and CAT enzymes decreased (p<0.05) by day 21 PS. Pearson's product-moment correlation showed a negative correlation between the levels of MDA and enzymatic antioxidant markers (CAT and GSH-Rd). Conclusion: The findings revealed that GSH-Rd and CAT are the primary antioxidant defense markers in donkey red blood cells. The observed alterations in these principal antioxidants suggest a 14 days optimum keeping time of donkey blood for blood banking purposes.

LACK OF EFFECT OF CORTICOSTEROIDS ON THE IN VIVO DISAPPEARANCE OF SULFHYDRYL-INHIBITED AND ANTIBODY-COATED ERYTHROCYTES

1964 ◽  
Vol 47 (3_Suppl) ◽  
pp. S28-S36
Author(s):  
Kailash N. Agarwal
Keyword(s):  
Red Cells ◽  
List Type ◽  
Red Cell

ABSTRACT Red cells were incubated in vitro with sulfhydryl inhibitors and Rhantibody with and without prior incubation with prednisolone-hemisuccinate. These erythrocytes were labelled with Cr51 and P32 and their disappearance in vivo after autotransfusion was measured. Prior incubation with prednisolone-hemisuccinate had no effect on the rate of red cell disappearance. The disappearance of the cells was shown to take place without appreciable intravascular destruction.

IN VITRO UPTAKE OF RADIOACTIVE 131I LABELLED L-TRIIODOTHYRONINE BY HUMAN ERYTHROCYTES AS AN INDEX OF THYROID FUNCTION

1960 ◽  
Vol XXXIV (II) ◽  
pp. 305-311 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos
Keyword(s):  
Thyroid Function ◽  
Incubation Time ◽  
Clinical Results ◽  
Human Erythrocytes ◽  
Red Cell ◽  
Clinical Value ◽  
Blood Samples ◽  
In Vitro Uptake

ABSTRACT The red cell triiodothyronine uptake technique as used in our hospital is described. Incubation time is of almost no importance. The temperature during incubation should be 37° C. Further improvement of the technique is obtained when all blood samples are brought up to 40 % haematocrit prior to incubation. Clinical results are discussed. It is yet too early to give a definite assessment of its clinical value, but it is definitely superior to the measurement of the BMR.

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