Genetic diversity of hepatitis D virus genotype‐1 in Europe allows classification into subtypes

2019 ◽  
Vol 26 (7) ◽  
pp. 900-910 ◽  
Author(s):  
Hadi Karimzadeh ◽  
Zainab Usman ◽  
Dmitrij Frishman ◽  
Michael Roggendorf
Keyword(s):  
Genetic Diversity ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus

scholarly journals Complete Genome Sequence of a Hepatitis D Virus Genotype 1 Strain Isolated in Guangdong, China

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Baolin Liao ◽  
Weilie Chen ◽  
Qu Ping ◽  
Haiyan Shi ◽  
Haolan He ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mainland China ◽  
Guangdong Province ◽  
Sequence Information ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus

Here, we report the first complete genome sequence of a hepatitis D virus genotype 1 strain, GZ37, isolated in Guangzhou, Guangdong Province, China, in 2014. The sequence information provided here will help us understand the molecular epidemiology of hepatitis D virus and contribute to disease control in mainland China.

scholarly journals Alpha Interferon and Ribavirin Combination Therapy of Chronic Hepatitis D

2005 ◽  
Vol 49 (3) ◽  
pp. 1135-1138 ◽  
Author(s):  
Sabahattin Kaymakoglu ◽  
Cetin Karaca ◽  
Kadir Demir ◽  
Sule Poturoglu ◽  
Ahmet Danalioglu ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Combination Therapy ◽  
Alpha Interferon ◽  
Virus Genotype ◽  
Genotype I ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Biochemical Responses ◽  
End Of Treatment

ABSTRACT The success of alpha interferon (IFN-α) monotherapy for the treatment of chronic hepatitis D is very limited. In this study, the efficacy of IFN-α and ribavirin combination therapy for chronic hepatitis D was investigated. Nineteen patients (15 males; mean age ± standard deviation, 36.8 ± 12.8 years) with chronic hepatitis D who were treated with IFN-α2b (10 million U, three times/week, subcutaneously) and ribavirin (1,000 to 1,200 mg/day, orally) for 24 months were studied. All patients had compensated liver disease (15 were precirrhotic), elevated transaminase levels, and hepatitis D virus RNA positivity at baseline. Genotypic analyses revealed hepatitis D virus genotype I and hepatitis B virus genotype D. All patients completed the 24 months of treatment and at least 6 months (7 to 19 months) of a follow-up period. Biochemical responses were observed in eight patients (42.1%) at the end of treatment and in seven patients (36.8%) at the end of follow-up. Eight patients (42.1%) at the end of treatment and four patients (21%) at the end of follow-up had virological responses. In conclusion, combination treatment of IFN-α and ribavirin for chronic hepatitis D is not able to induce virological responses at a sufficient rate, despite its partial effectiveness in improving biochemical responses, and is not superior to IFN-α monotherapy.

scholarly journals Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

2015 ◽  
Vol 53 (4) ◽  
pp. 1164-1171 ◽  
Author(s):  
Marie-Bernadette Villiers ◽  
Jean-Claude Cortay ◽  
Sandra Cortès ◽  
Bénédicte Bloquel ◽  
Ségolène Brichler ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Hepatitis B ◽  
Recombinant Proteins ◽  
Surface Plasmon Resonance Imaging ◽  
Genotype 1 ◽  
Resonance Imaging ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Hdv Infection ◽  
Hdv Genotype

Liver diseases linked to hepatitis B-hepatitis D virus co- or superinfections are more severe than those during hepatitis B virus (HBV) monoinfection. The diagnosis of hepatitis D virus (HDV) infection therefore remains crucial in monitoring patients but is often overlooked. To integrate HDV markers into high-throughput viral hepatitis diagnostics, we studied the binding of anti-HDV antibodies (Abs) using surface plasmon resonance imaging (SPRi). We focused on the ubiquitous HDV genotype 1 (HDV1) and the more uncommon African-HDV6 and HDV8 genotypes to define an array with recombinant proteins or peptides. Full-length and truncated small hepatitis D antigen (S-HDAg) recombinant proteins of HDV genotype 1 (HDV1) and 11 HDV peptides of HDV1, 6, and 8, representing various portions of the delta antigen were grafted onto biochips, allowing SPRi measurements to be made. Sixteen to 17 serum samples from patients infected with different HDV genotypes were injected onto protein and peptide chips. In all, Abs against HDV proteins and/or peptides were detected in 16 out of 17 infected patients (94.12%), although the amplitude of the SPR signal varied. The amino-terminal part of the protein was poorly immunogenic, while epitope 65-80, exposed on the viral ribonucleoprotein, may be immunodominant, as 9 patient samples led to a specific SPR signal on peptide 65 type 1 (65#1), independently of the infecting genotype. In this pilot study, we confirmed that HDV infection screening based on the reactivity of patient Abs against carefully chosen HDV peptides and/or proteins can be included in a syndrome-based viral hepatitis diagnostic assay. The preliminary results indicated that SPRi studying direct physical HDAg–anti-HDV Ab interactions was more convenient using linear peptide epitopes than full-length S-HDAg proteins, due to the regeneration process, and may represent an innovative approach for a hepatitis syndrome–viral etiology-exploring array.

scholarly journals Reply to the Letter of Charre et al. “Mis-Genotyping of Some Hepatitis D Virus Genotype 2 and 5 Sequences Using HDVdb”

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1278
Author(s):  
Zainab Usman ◽  
Stoyan Velkov ◽  
Ulrike Protzer ◽  
Michael Roggendorf ◽  
Dmitrij Frishman ◽  
...  
Keyword(s):  
Human Error ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Genotype 2

We thank Charre and colleagues for spotting the mis-annotation of sequences in our database, which was caused by human error [...]

scholarly journals Hepatitis B Surface Antigen Levels and Sequences of Natural Hepatitis B Virus Variants Influence the Assembly and Secretion of Hepatitis D Virus

2007 ◽  
Vol 82 (5) ◽  
pp. 2250-2264 ◽  
Author(s):  
Hsuan Hui Shih ◽  
King-Song Jeng ◽  
Wan-Jr Syu ◽  
Yi-Hsiang Huang ◽  
Chien-Wei Su ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Genotype 1 ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
B Virus ◽  
Expression Plasmids

ABSTRACT Various domains of hepatitis B surface antigen (HBsAg) are essential for the assembly and secretion of hepatitis D virus (HDV). This study investigated the influences of the levels and sequences of HBsAg of naturally occurring HBV variants on the assembly and secretion of HDV. Six hepatitis B virus (HBV)-producing plasmids (three genotype B and three genotype C) and six HBsAg expression plasmids that expressed various HBsAg levels were constructed from the sera of HDV-infected patients. These plasmids were cotransfected with six expression plasmids of HDV of genotype 1, 2, or 4 into the Huh-7 hepatoma cell line. Serum HBsAg and HBV DNA levels were correlated with HDV RNA levels and outcomes of chronic hepatitis D (CHD) patients. The secretion of genotype 1, 2, or 4 HDV generally correlated with HBsAg levels but not with HBV genotypes or HBV DNA levels. Swapping and residue mutagenesis experiments of HBsAg-coding sequences revealed that the residue Pro-62 in the cytosolic domain-I affects the assembly and secretion of genotype 2 and 4 HDV and not those of genotype 1. The pre-S2 N-terminal deletion HBV mutant adversely affects secretion of the three HDV genotypes. In patients, serum HDV RNA levels correlated with HBsAg levels but not with HBV DNA levels. Viremia of HDV or HBV correlated with poor outcomes. In conclusion, the assembly and secretion of HDV were influenced by the amounts and sequences of HBsAg. For an effective treatment of CHD, reduction of HBsAg production in addition to the suppression of HBV and HDV replication might be crucial.

scholarly journals Novel Synthesis and Phenotypic Analysis of Mutant Clouds for Hepatitis E Virus Genotype 1

2017 ◽  
pp. JVI.01932-17 ◽  
Author(s):  
Shubhra Agarwal ◽  
Prasith Baccam ◽  
Rakesh Aggarwal ◽  
Naga Suresh Veerapu
Keyword(s):  
Genetic Diversity ◽  
Cell Culture ◽  
Hepatitis E Virus ◽  
Rna Viruses ◽  
Hepatitis E ◽  
Replication Capacity ◽  
Genotype 1 ◽  
Wild Type ◽  
Virus Genotype ◽  
Cross Sectional

Many RNA viruses exist as an ensemble of genetically-diverse, replicating populations, known as mutant cloud. The genetic diversity (cloud size) and composition of this mutant cloud may influence several important phenotypic features of the virus, including its replication capacity. We applied a straightforward, bacterium-free approach using error-prone PCR coupled with reverse genetics to generate infectious mutant RNA clouds of varying genetic diversity from a genotype 1 strain of hepatitis E virus (HEV). Cloning and sequencing of a genomic fragment encompassing 70% of open reading frame 1 (ORF1) or of the full-genome from variants in the resultant clouds showed the occurrence of nucleotide mutations at a frequency of the order of 10-3per nucleotide copied, and existence of marked genetic diversity with a high normalized Shannon entropy. The mutant clouds showed transient replication in cell culture, while wild-type HEV did not. Cross-sectional data from these cell cultures supported the existence of differential effects of clouds of varying sizes and compositions on phenotypic characteristics such as the replication level of (+)-RNA progeny, amount of double-stranded RNA (a surrogate for rate of viral replication) and ORF1 protein, and expression of interferon stimulating genes. Since mutant cloud size and composition influenced the viral phenotypic properties, a better understanding of this relationship may help provide further insights into virus evolution and prediction of emerging viral diseases.IMPORTANCESeveral biological or practical limitations currently prevent the study of phenotypic behavior of a mutant cloudin vitro. We developed a simple and rapid method for synthesizing mutant clouds of hepatitis E virus (HEV), a ss(+)RNA virus, with varying and controllable levels of genetic diversity, which could then be used in a cell culture system to study the effect of cloud size and composition on viral phenotype. In a cross-sectional analysis, we demonstrated that a particular mutant cloud, which had an extremely highgenetic diversity, had replication rate exceeding that of the wild-type HEV. This method should thus provide a useful model for understanding the phenotypic behavior of ss(+) RNA viruses.

scholarly journals Characterization of hepatitis D virus genotype III among Yucpa Indians in Venezuela

2001 ◽  
Vol 82 (9) ◽  
pp. 2183-2189 ◽  
Author(s):  
Tatsunori Nakano ◽  
Craig N. Shapiro ◽  
Stephan C. Hadler ◽  
John L. Casey ◽  
Masashi Mizokami ◽  
...  
Keyword(s):  
Hypervariable Region ◽  
Editing Site ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Genotype Iii ◽  
D Antigen ◽  
Hdv Genotype

The complete genome sequences of hepatitis D virus (HDV) strains isolated from three Yucpa Amerindians in Venezuela were determined and found to be genotype III. Comparison of these three genotype III sequences demonstrated the presence of a hypervariable region containing numerous substitutions, insertions/deletions and a highly conserved region containing the self-cleavage domains, which have been reported previously for genotypes I and II. Amino acid changes within the first 90 amino acids of the hepatitis D antigen (HDAg) were found in the genotype III sequences, while the remainder of the HDAg-coding sequence was conserved. The secondary structure for the RNA-editing site differed between genotypes I and III. It was concluded that the serious delta hepatitis outbreaks characterized epidemiologically in the Yucpa Amerindians were caused by HDV genotype III isolates that were related to HDV genotype III isolates from other regions of South America.

scholarly journals Mis-Genotyping of Some Hepatitis D Virus Genotype 2 and 5 Sequences Using HDVdb

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1066
Author(s):  
Caroline Charre ◽  
Frédéric le Gal ◽  
Paul Dény ◽  
Caroline Scholtès
Keyword(s):  
Liver Fibrosis ◽  
Treatment Response ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Beneficial Use ◽  
Hepatitis D Virus ◽  
Genotype 2 ◽  
Hdv Infection ◽  
Beta Version ◽  
Hdv Genotype

Evidence that Hepatitis D virus (HDV) genotype is involved in HDV infection pathogenesis is increasing. Indeed, HDV genotypes have been shown to be linked to different outcomes in terms of liver fibrosis and treatment response. Herein, we show that the promising HDVdb genotyping tool available online can lead to wrong genotyping results. The current HDVdb algorithm should be carefully considered as a “beta-version” and warrants algorithm core corrections, as soon as possible, for an optimal and beneficial use.

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