scholarly journals Mis-Genotyping of Some Hepatitis D Virus Genotype 2 and 5 Sequences Using HDVdb

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1066
Author(s):  
Caroline Charre ◽  
Frédéric le Gal ◽  
Paul Dény ◽  
Caroline Scholtès
Keyword(s):  
Liver Fibrosis ◽  
Treatment Response ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Beneficial Use ◽  
Hepatitis D Virus ◽  
Genotype 2 ◽  
Hdv Infection ◽  
Beta Version ◽  
Hdv Genotype

Evidence that Hepatitis D virus (HDV) genotype is involved in HDV infection pathogenesis is increasing. Indeed, HDV genotypes have been shown to be linked to different outcomes in terms of liver fibrosis and treatment response. Herein, we show that the promising HDVdb genotyping tool available online can lead to wrong genotyping results. The current HDVdb algorithm should be carefully considered as a “beta-version” and warrants algorithm core corrections, as soon as possible, for an optimal and beneficial use.

scholarly journals Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

2015 ◽  
Vol 53 (4) ◽  
pp. 1164-1171 ◽  
Author(s):  
Marie-Bernadette Villiers ◽  
Jean-Claude Cortay ◽  
Sandra Cortès ◽  
Bénédicte Bloquel ◽  
Ségolène Brichler ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Hepatitis B ◽  
Recombinant Proteins ◽  
Surface Plasmon Resonance Imaging ◽  
Genotype 1 ◽  
Resonance Imaging ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Hdv Infection ◽  
Hdv Genotype

Liver diseases linked to hepatitis B-hepatitis D virus co- or superinfections are more severe than those during hepatitis B virus (HBV) monoinfection. The diagnosis of hepatitis D virus (HDV) infection therefore remains crucial in monitoring patients but is often overlooked. To integrate HDV markers into high-throughput viral hepatitis diagnostics, we studied the binding of anti-HDV antibodies (Abs) using surface plasmon resonance imaging (SPRi). We focused on the ubiquitous HDV genotype 1 (HDV1) and the more uncommon African-HDV6 and HDV8 genotypes to define an array with recombinant proteins or peptides. Full-length and truncated small hepatitis D antigen (S-HDAg) recombinant proteins of HDV genotype 1 (HDV1) and 11 HDV peptides of HDV1, 6, and 8, representing various portions of the delta antigen were grafted onto biochips, allowing SPRi measurements to be made. Sixteen to 17 serum samples from patients infected with different HDV genotypes were injected onto protein and peptide chips. In all, Abs against HDV proteins and/or peptides were detected in 16 out of 17 infected patients (94.12%), although the amplitude of the SPR signal varied. The amino-terminal part of the protein was poorly immunogenic, while epitope 65-80, exposed on the viral ribonucleoprotein, may be immunodominant, as 9 patient samples led to a specific SPR signal on peptide 65 type 1 (65#1), independently of the infecting genotype. In this pilot study, we confirmed that HDV infection screening based on the reactivity of patient Abs against carefully chosen HDV peptides and/or proteins can be included in a syndrome-based viral hepatitis diagnostic assay. The preliminary results indicated that SPRi studying direct physical HDAg–anti-HDV Ab interactions was more convenient using linear peptide epitopes than full-length S-HDAg proteins, due to the regeneration process, and may represent an innovative approach for a hepatitis syndrome–viral etiology-exploring array.

scholarly journals Reply to the Letter of Charre et al. “Mis-Genotyping of Some Hepatitis D Virus Genotype 2 and 5 Sequences Using HDVdb”

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1278
Author(s):  
Zainab Usman ◽  
Stoyan Velkov ◽  
Ulrike Protzer ◽  
Michael Roggendorf ◽  
Dmitrij Frishman ◽  
...  
Keyword(s):  
Human Error ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Genotype 2

We thank Charre and colleagues for spotting the mis-annotation of sequences in our database, which was caused by human error [...]

scholarly journals Characterization of hepatitis D virus genotype III among Yucpa Indians in Venezuela

2001 ◽  
Vol 82 (9) ◽  
pp. 2183-2189 ◽  
Author(s):  
Tatsunori Nakano ◽  
Craig N. Shapiro ◽  
Stephan C. Hadler ◽  
John L. Casey ◽  
Masashi Mizokami ◽  
...  
Keyword(s):  
Hypervariable Region ◽  
Editing Site ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Genotype Iii ◽  
D Antigen ◽  
Hdv Genotype

The complete genome sequences of hepatitis D virus (HDV) strains isolated from three Yucpa Amerindians in Venezuela were determined and found to be genotype III. Comparison of these three genotype III sequences demonstrated the presence of a hypervariable region containing numerous substitutions, insertions/deletions and a highly conserved region containing the self-cleavage domains, which have been reported previously for genotypes I and II. Amino acid changes within the first 90 amino acids of the hepatitis D antigen (HDAg) were found in the genotype III sequences, while the remainder of the HDAg-coding sequence was conserved. The secondary structure for the RNA-editing site differed between genotypes I and III. It was concluded that the serious delta hepatitis outbreaks characterized epidemiologically in the Yucpa Amerindians were caused by HDV genotype III isolates that were related to HDV genotype III isolates from other regions of South America.

Chronic Hepatitis D Virus (HDV) Infection in Hepatitis B Virus Carrier Chimpanzees Experimentally Superinfected with HDV

1988 ◽  
Vol 158 (1) ◽  
pp. 151-159 ◽  
Author(s):  
F. Negro ◽  
K. F. Bergmann ◽  
B. M. Baroudy ◽  
W. C. Satterfield ◽  
H. Popper ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
B Virus ◽  
Hepatitis B Virus Carrier ◽  
Hdv Infection ◽  
Virus Carrier

Epidemiology of hepatitis D virus (HDV) infection in an urban area of Northern Italy

Infection ◽  
2012 ◽  
Vol 40 (5) ◽  
pp. 485-491 ◽  
Author(s):  
M. De Paschale ◽  
M. T. Manco ◽  
L. Belvisi ◽  
C. Magnani ◽  
T. Re ◽  
...  
Keyword(s):  
Urban Area ◽  
Northern Italy ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Hdv Infection

Comparison of anti–hepatitis D virus (HDV) ETI-AB-DELTAK-2 assay and the novel LIAISON® XL MUREX anti-HDV assay in the diagnosis of HDV infection

2019 ◽  
Vol 95 (4) ◽  
pp. 114873 ◽  
Author(s):  
Caterina Rocco ◽  
Raffaele Bonavolta ◽  
Luca Vallefuoco ◽  
Umberto Braschi ◽  
Rosanna Sorrentino ◽  
...  
Keyword(s):  
The Novel ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
Hdv Infection

scholarly journals Hepatitis D virus antibodies and liver function profile among patients with chronic hepatitis B infection in Abuja, Nigeria

2021 ◽  
Vol 15 (01) ◽  
pp. 141-146
Author(s):  
Lukman O Abdulkareem ◽  
Dennis A Ndububa ◽  
Augustine O Uhunmwangho ◽  
Thahir Yunusa
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Liver Function ◽  
Chronic Hepatitis B ◽  
Prevalence Rate ◽  
Cross Sectional ◽  
Hepatitis D ◽  
Hepatitis D Virus ◽  
The Mean ◽  
Hdv Infection

Introduction: Hepatitis D virus (HDV) is a satellite virus of hepatitis B virus (HBV). An estimated 5% of HBV infected individuals worldwide have HDV infection. There is paucity of studies in Nigeria on the burden of HDV infection. This study aimed at determining the prevalence rate of HDV antibodies among individuals with chronic hepatitis B (CHB) infection and comparing the liver function test (LFT) and disease severity among the anti-HDV positive (anti-HDV+) and anti-HDV negative (anti-HDV-) individuals. Methodology: A cross-sectional study of 180 CHB infected individuals who were clinically evaluated and tested for HDV antibodies using the Enzyme-linked Immunoassay method. Their LFT profile and Child-Turcotte-Pugh (CTP) were also assessed. Data were analyzed using the SPSS version 17. Results: Their mean age was 35.2 ± 10.4 years. There were 150 (83.3%) and 30 (16.7%) individuals with uncomplicated and complicated CHB infection respectively. Thirty-four (18.9%) of the participants were anti-HDV+. The mean serum ALT, AST, albumin and INR of the anti-HDV+ subjects were 16.5 ± 13.8 IU/L, 26.3 ± 32.6 IU/L, 38.9 ± 7.6 g/L, and 1.2 ± 0.2 respectively. The mean values for the same parameters of the anti-HDV- subjects were 10.8 ± 9.5 IU/L, 13.4 ± 11.2 IU/L, 41.4 ± 6.0 g/L and 1.1 ± 0.2 respectively (p < 0.05). The mean CTP scores in the anti-HDV+ and anti-HDV- subjects were 6.1 ± 2.1 and 5.5 ± 1.2 respectively (p= 0.03). Conclusions: Anti-HDV sero-prevalence rate was 18.9% and anti-HDV+ CHB patients had worse LFT results compared to those who were anti-HDV–.

scholarly journals Characterization of the Full-Length Genome Sequences and Phylogenetic Analysis of HDV Strains Isolated from Patients with Chronic HBV and HDV Infection in Kyrgyz Republic

2020 ◽  
pp. 124-132
Author(s):  
Yu. V. Ostankova ◽  
K. A. Nogoybaeva ◽  
E. B. Zueva ◽  
K. T. Kasymbekova ◽  
S. T. Tobokalova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Molecular Genetic ◽  
Kyrgyz Republic ◽  
Genotype 1 ◽  
Hepatitis D ◽  
Hdv Infection ◽  
Molecular Genetic Characterization ◽  
Hdv Genotype

Objective. The purpose of our work was molecular genetic characterization of the hepatitis D virus isolates, circulating in the region with high prevalence of HBV + HDV super-infection. Materials and methods. The study material was 64 blood serum samples obtained from Kyrgyz Republic residents - patients with chronic viral hepatitis B+D. The hepatitis D virus complete genomes were sequenced, followed by phylogenetic analysis. Results and discussion. Based on the phylogenetic analysis of 64 HDV samples, it was shown that HDV genotype 1 (96.9 %) predominates in the examined group compared with HDV genotype 2 (3.1 %). Sequences were submitted to GenBank under access No MN984407 through MN984470. When assessing the genetic variability over the examined HDV genotype 1 samples, the maximum genetic distance was 12,49 %, and the minimum – 7,41 %. Within individual clusters, the genetic distance averaged from 2.6 % to 8.5 %. Among the sequences in GenBank, the closest resemblance to the HDV-2 Kyr41 and Kyr43 samples (nucleotide identity was 92.31 % and 89.57 %, respectively) was shown for the virus described earlier in Yakutia (AJ309880). To study the genetic relationships between the analyzed HDV genotype 1 strains in comparison with the HDV reference sequences, the predicted amino acid sequence was studied (111–214). Although hepatitis B preventive measures, including vaccination, have reduced the hepatitis D incidence, there is no effective way to prevent HDV infection in HBV carriers in endemic areas. The HDV sequence molecular-genetic characterization in this study, as well as the viral genomic sequence phylogenetic analysis, will help identify pathogen transmission pathways to control and / or prevent the spread of infection.

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