In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures

2013 ◽  
Vol 49 (3) ◽  
pp. 371-381 ◽  
Author(s):  
A. M. Pabst ◽  
A. Happe ◽  
A. Callaway ◽  
T. Ziebart ◽  
S. I. Stratul ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
In Vivo Characterization ◽  
Acellular Dermal

scholarly journals Non-Cross-Linked Collagen Mesh Performs Best in a Physiologic, Noncontaminated Rat Model

2019 ◽  
Vol 26 (3) ◽  
pp. 302-311 ◽  
Author(s):  
Ruth Kaufmann ◽  
An P. Jairam ◽  
Irene M. Mulder ◽  
Zhouqiao Wu ◽  
Joost Verhelst ◽  
...  
Keyword(s):  
Rat Model ◽  
Incisional Hernia Repair ◽  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Laparoscopic Incisional Hernia Repair ◽  
Mesh Shrinkage ◽  
Male Wistar Rats ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

Background. In laparoscopic incisional hernia repair, direct contact between the prosthesis and abdominal viscera is inevitable and may lead to adhesions. Despite the large variety of mesh prosthesis, little is known about their in vivo behavior. Biological meshes are considered to have many advantages, but due to their price they are rarely used. A rat model was used to assess biological and conventional synthetic meshes on their in vivo characteristics. Design. One-hundred twenty male Wistar rats were randomized into five groups of 24 rats. A mesh was implanted intraperitoneally and fixated with nonresorbable sutures. The following five meshes were implanted: Parietene (polypropylene), Permacol (cross-linked porcine acellular dermal matrix), Strattice (non-cross-linked porcine acellular dermal matrix), XCM Biologic (non-cross-linked porcine acellular dermal matrix), and Omyra Mesh (condensed polytetrafluoroethylene). The rats were sacrificed after 30, 90, or 180 days. Incorporation, shrinkage, adhesions, abscess formation, and histology were assessed for all meshes. Results. All animals thrived postoperatively. After 180 days, Permacol, Parietene, and Omyra Mesh had a significantly better incorporation than Strattice ( P = .001, P = .019, and P = .037 respectively). After 180 days, Strattice had significantly fewer adhesions on the surface of the mesh than Parietene ( P < .001), Omyra Mesh ( P = .011), and Permacol ( P = .027). After 30 days, Permacol had significantly stronger adhesions than Strattice ( P = .030). However, this difference was not significant anymore after 180 days. After 180 days, there was significantly less shrinkage in Permacol than in Strattice ( P = .001) and Omyra Mesh ( P = .050). Conclusion. Based on incorporation, adhesions, mesh shrinkage, and histologic parameters, Strattice performed best in this experimental rat model.

Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

2019 ◽  
Vol 14 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Zhu Zhu ◽  
Zhao‐Qi Yuan ◽  
Cheng Huang ◽  
Rui Jin ◽  
Di Sun ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Acellular Dermal

scholarly journals Biocompatibility Evaluation of Human and Porcine Acellular Dermal Matrix on Human Primary Gingival Fibroblasts: In Vitro Comparative Study

2021 ◽  
Author(s):  
Ehab Azab ◽  
Abdel-Rahman Youssef
Keyword(s):  
Cell Viability ◽  
Acellular Dermal Matrix ◽  
P Value ◽  
Gingival Fibroblasts ◽  
Dermal Matrix ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

Abstract Objective Allogeneic and xenogeneic acellular dermal matrix (ADM) grafts have been used to treat periodontal soft tissue defects. The purpose of the current study was to compare the effect of human ADM (AlloDerm) and porcine ADM (Derma) on human primary gingival fibroblasts in vitro regarding the biocompatibility test. Materials and Methods Gingival fibroblasts were obtained from healthy adult gingiva and seeded on AlloDerm or Derma ADM in 96-well plate. The control cells were grown on a surface-treated polystyrene cell-culture plate without matrix. The cells were cultured for 3, 7, and 14 days. The fibroblasts morphology was examined using inverted microscopy, and the cell viability of fibroblasts adherent to the dermal matrix was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay after 3, 7, and 14 days in culture. The data were statistically evaluated by one-way analysis of variance. p-Value of 0.05 was considered significant. Results Gingival fibroblasts adjacent to the AlloDerm and Derma matrices were healthy, attached to the well, and did not exhibit any cytopathic changes similar to control. There were no statistically significant differences in the cell viability between the gingival fibroblasts attached to Derma and AlloDerm on day 3 (p = 0.841), day 7 (p = 0.198), and day 14 (p = 0.788). Conclusion Considering this in vitro study’s limitations, both human and porcine ADM were compatible with the surrounding human primary gingival fibroblasts. No significant differences were observed in the cell viability between the gingival fibroblasts that were attached to Derma and AlloDerm.

scholarly journals Effect of Growth Factor-Loaded Acellular Dermal Matrix/MSCs on Regeneration of Chronic Tympanic Membrane Perforations in Rats

2021 ◽  
Vol 10 (7) ◽  
pp. 1541
Author(s):  
Gwang-Won Cho ◽  
Changjong Moon ◽  
Anji Song ◽  
Karthikeyan A. Vijayakumar ◽  
Mary Jasmin Ang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Success Rate ◽  
Tympanic Membrane ◽  
Acellular Dermal Matrix ◽  
In Vivo Studies ◽  
Dermal Matrix ◽  
Tympanic Membrane Perforations ◽  
Acellular Dermal

The success rate of grafting using acellular dermal matrix (ADM) for chronic tympanic membrane was reported in previous studies to be lower than fascia or perichondrium. Combining mesenchymal stem cells (MSCs) and growth factor-loaded ADM for the regeneration of chronic TMP has not been reported so far. In this study, we hypothesized that combining growth factor-loaded ADM/MSCs could promote the recruitment of MSCs and assist in TMP regeneration. We evaluated the regeneration and compared the performance of four scaffolds in both in vitro and in vivo studies. MTT, qPCR, and immunoblotting were performed with MSCs. In vivo study was conducted in 4 groups (control; ADM only, ADM/MSC, ADM/MSC/bFGF, ADM/MSC/EGF) of rats and inferences were made by otoendoscopy and histological changes. Attachment of MSCs on ADM was observed by confocal microscopy. Proliferation rate increased with time in all treated cells. Regeneration-related gene expression in the treated groups was higher. Also, graft success rate was significantly higher in ADM/MSC/EGF group than other groups. Significant relationships were disclosed in neodrum thickness between each group. The results suggest, in future, combining EGF with ADM/MSCs could possibly be used as an outpatient treatment, without the need for surgery for eardrum regeneration.

Effect of In Vitro Gingival Fibroblast Seeding on the In Vivo Incorporation of Acellular Dermal Matrix Allografts in Dogs

2007 ◽  
Vol 78 (2) ◽  
pp. 296-303 ◽  
Author(s):  
Arthur B. Novaes ◽  
Julie Teresa Marchesan ◽  
Guilherme O. Macedo ◽  
Daniela B. Palioto
Keyword(s):  
Acellular Dermal Matrix ◽  
Gingival Fibroblast ◽  
Dermal Matrix ◽  
Acellular Dermal
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