Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

2019 ◽  
Vol 14 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Zhu Zhu ◽  
Zhao‐Qi Yuan ◽  
Cheng Huang ◽  
Rui Jin ◽  
Di Sun ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Acellular Dermal

In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures

2013 ◽  
Vol 49 (3) ◽  
pp. 371-381 ◽  
Author(s):  
A. M. Pabst ◽  
A. Happe ◽  
A. Callaway ◽  
T. Ziebart ◽  
S. I. Stratul ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
In Vivo Characterization ◽  
Acellular Dermal

scholarly journals Effect of Growth Factor-Loaded Acellular Dermal Matrix/MSCs on Regeneration of Chronic Tympanic Membrane Perforations in Rats

2021 ◽  
Vol 10 (7) ◽  
pp. 1541
Author(s):  
Gwang-Won Cho ◽  
Changjong Moon ◽  
Anji Song ◽  
Karthikeyan A. Vijayakumar ◽  
Mary Jasmin Ang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Success Rate ◽  
Tympanic Membrane ◽  
Acellular Dermal Matrix ◽  
In Vivo Studies ◽  
Dermal Matrix ◽  
Tympanic Membrane Perforations ◽  
Acellular Dermal

The success rate of grafting using acellular dermal matrix (ADM) for chronic tympanic membrane was reported in previous studies to be lower than fascia or perichondrium. Combining mesenchymal stem cells (MSCs) and growth factor-loaded ADM for the regeneration of chronic TMP has not been reported so far. In this study, we hypothesized that combining growth factor-loaded ADM/MSCs could promote the recruitment of MSCs and assist in TMP regeneration. We evaluated the regeneration and compared the performance of four scaffolds in both in vitro and in vivo studies. MTT, qPCR, and immunoblotting were performed with MSCs. In vivo study was conducted in 4 groups (control; ADM only, ADM/MSC, ADM/MSC/bFGF, ADM/MSC/EGF) of rats and inferences were made by otoendoscopy and histological changes. Attachment of MSCs on ADM was observed by confocal microscopy. Proliferation rate increased with time in all treated cells. Regeneration-related gene expression in the treated groups was higher. Also, graft success rate was significantly higher in ADM/MSC/EGF group than other groups. Significant relationships were disclosed in neodrum thickness between each group. The results suggest, in future, combining EGF with ADM/MSCs could possibly be used as an outpatient treatment, without the need for surgery for eardrum regeneration.

Effect of In Vitro Gingival Fibroblast Seeding on the In Vivo Incorporation of Acellular Dermal Matrix Allografts in Dogs

2007 ◽  
Vol 78 (2) ◽  
pp. 296-303 ◽  
Author(s):  
Arthur B. Novaes ◽  
Julie Teresa Marchesan ◽  
Guilherme O. Macedo ◽  
Daniela B. Palioto
Keyword(s):  
Acellular Dermal Matrix ◽  
Gingival Fibroblast ◽  
Dermal Matrix ◽  
Acellular Dermal

Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

2009 ◽  
Vol 610-613 ◽  
pp. 1298-1301
Author(s):  
Xiang Rong Zhang ◽  
De Wu Liu ◽  
Guang Hua Guo ◽  
Yan Peng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gradient Centrifugation ◽  
Human Mesenchymal Stem Cells ◽  
Acellular Dermal Matrix ◽  
Culture Method ◽  
Gene Encoding ◽  
Dermal Matrix ◽  
Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

scholarly journals Three-dimensional cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds and their biocompatibility evaluation in vitro

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 15926-15935 ◽  
Author(s):  
Peng Liu ◽  
Qing Li ◽  
Qiaolin Yang ◽  
Shihan Zhang ◽  
Chunping Lin ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Three Dimensional ◽  
Acellular Dermal Matrix ◽  
Biological Properties ◽  
Gingival Fibroblast ◽  
Dermal Matrix ◽  
Cell Printing ◽  
Acellular Dermal ◽  
Dimensional Cell

3D cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds showed satisfactory biological properties.

scholarly journals In Vitro Analysis of Histology, Mechanics, and Safety of Radiation-free Pre-hydrated Human Acellular Dermal Matrix

2020 ◽  
Vol 23 (6) ◽  
pp. 635
Author(s):  
Ji Young Kim ◽  
Kyung Min Yang ◽  
Ji Hyun Youn ◽  
Heejun Park ◽  
Hyung Min Hahn ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Vitro Analysis ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix ◽  
In Vitro Analysis
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